FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003630825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003630825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257991
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC17420.6752173525433057No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13640.5287006135872957No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG13440.5209484051769248No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA10570.40970421448810235No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA10140.3930369664058049No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9400.3643537952874325No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8680.3364458450100972No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8340.32326709071246673No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA8320.32249186987142964No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT7610.2949715300146129No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG7540.2922582570709831No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT6760.26202464427053657No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG6530.25310960459861004No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6510.25233438375757294No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6490.25155916291653585No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG6470.25078394207549876No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC6270.24303173366512784No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT6170.23915562945994237No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT5900.22869014810594168No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT5800.2248140439007562No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC5770.22365121263920057No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG5600.21706183549038532No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC5450.21124767918260715No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA5440.2108600687620886No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT5290.20504591245431042No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT5110.19806892488497663No Hit
TTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCT5030.19496804152082825No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG4870.18876627479253152No Hit
TCCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGAC4740.18372733932579044No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG4730.1833397289052719No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC4700.18217689764371625No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT4670.1810140663821606No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA4660.1806264559616421No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA4590.17791318301801226No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG4580.1775255725974937No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG4460.17287424755127115No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT4370.16938575376660425No Hit
CTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTC4360.1689981433460857No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC4300.16667248082297445No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC4300.16667248082297445No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA4150.16085832451519627No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT4140.16047071409467772No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA4140.16047071409467772No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA4110.15930788283312208No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG4070.15775744115104792No Hit
CTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGA4070.15775744115104792No Hit
GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG4050.1569822203100108No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA4040.15659460988949228No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC3910.15155567442275117No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC3850.1492300118996399No Hit
GTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTT3650.141477803489269No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA3600.13953975138667626No Hit
GTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGT3600.13953975138667626No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT3580.13876453054563917No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG3540.137214088863565No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT3310.12829904919163848No Hit
TCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAG3310.12829904919163848No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT3290.1275238283506014No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG3290.1275238283506014No Hit
ATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCA3200.12403533456593448No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG3180.12326011372489737No Hit
GGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCC3170.12287250330437884No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC3070.11899639909919338No Hit
CCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGACA3060.11860878867867483No Hit
GTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTA3050.1182211782581563No Hit
CACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGT3050.1182211782581563No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC3020.11705834699660067No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA3020.11705834699660067No Hit
CTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACG3000.11628312615556356No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG3000.11628312615556356No Hit
GGCATAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTC2940.1139574636324523No Hit
ATATTAAGGTGTTCAGTTAAAATCTTTGAACCAAGCGCAATTGCTTCTTT2930.11356985321193375No Hit
ATTCTGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGT2910.11279463237089667No Hit
ATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTG2910.11279463237089667No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT2910.11279463237089667No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT2900.1124070219503781No Hit
GCATAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTCG2870.11124419068882246No Hit
GCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTC2860.11085658026830393No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG2860.11085658026830393No Hit
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTA2850.1104689698477854No Hit
GTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGT2840.11008135942726684No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG2780.10775569690415557No Hit
GCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTT2770.10736808648363703No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC2730.10581764480156283No Hit
ATTCAAATGAGCATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGA2730.10581764480156283No Hit
CTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGT2710.10504242396052577No Hit
GTACATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTC2700.1046548135400072No Hit
GCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAAAGGCTGAA2690.10426720311948867No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG2680.10387959269897012No Hit
TTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTAACG2680.10387959269897012No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA2660.10310437185793303No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC2640.10232915101689594No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC2630.10194154059637739No Hit
CCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGT2620.10155393017585886No Hit
ATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCT2580.10000348849378467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)