FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39
Sequences flagged as poor quality0
Sequence length100
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGTGGTCGAGCCGAGCGTCGTACAGGCCCGTGATCAGGTGCGCAGCA25.128205128205128No Hit
CGGCCAGGTCACGCTGGGCAACACCATCGTCAAGGCCAGCGCGCGCAAGG25.128205128205128No Hit
GTTCTGGACTGGCTGCGTGGTTCCGATGAGCCGGTGGTCAATGTCGACAA25.128205128205128No Hit
ACGGTGAGCCAAATGCCGACGGCGGTATAGTTGACCTAAACGAGGCAGAA25.128205128205128No Hit
TTCCAGGGCGTGACGGACGAGTTGGGCGCCGGTTTTTTTCATGCTGGTCT25.128205128205128No Hit
GAGCAGGCGCTGCAGCGGGTCAGCGCAGTGCCCTTCCTGGCGGCGGTGGA25.128205128205128No Hit
GCCTTCGAGATTGGCGTGACGCATGGCGTCCTGCAGAGCAAGCGCGTTCA25.128205128205128No Hit
CCAGTACTCCGAGCGGCTGGCGCTCCCGGAGAAATCGGCGTACTTGCTGA25.128205128205128No Hit
GACCCAATCTGCCACCGCCGATCGGGTTGACGCGTCGCCCTCTGGCCTGC25.128205128205128No Hit
ATTCGAGGCTGCTTCCTCCGCCTCACGCTTCACTTCAATCGTTAATTCCT25.128205128205128No Hit
GGTCAAGGGTTTCGGCCGCCAGAAGGGGCACACCGAGCTGTATCGCGGTG12.564102564102564No Hit
GCGGCAGTTGTACGCCCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGG12.564102564102564No Hit
TCTCCGAGCCCACGAGACCACATCTACGGTATCTCGTATGCCGTCTTCTG12.564102564102564RNA PCR Primer, Index 31 (96% over 25bp)
GGCCTTCGGGCACCCGCTGCCGTCCGGCGCGGCCGACCCGGTGTGACCGG12.564102564102564No Hit
GCCGTGGTCGCCGCCGCCGGCCGGCGCCTCGTCATCGACGCGATGAGCAG12.564102564102564No Hit
CACGTTCGAACGCCCGCAGCGTGAAGGCCGCGGGTTCGCCATGGGCAAGC12.564102564102564No Hit
GCCCTGGTGCTGTGCTTCAGCATCGGCCTGGCCGCGACCATGGTCGCGTC12.564102564102564No Hit
CGAATGGAGCCACCAACTGCTCAGCCGGCGCACGCGGCACGTGCTGCACC12.564102564102564No Hit
GGTATCCTCTAATCTGTTAATGGCCTAAACAGAACAAAAAGGTGGAGGAA12.564102564102564No Hit
AATATCACACAAGGGCCTGAAGCCTCAAACTTCAGCCCAGCATATTCAAA12.564102564102564No Hit
GGCACAGCCCGACCTGTTTGATATGCGGATAGCGGGCATACATCGCCCAG12.564102564102564No Hit
ACGTCGATGAGCGTGCCGTCCGCAATCGCCGACGACAGCCCCGCGTACAC12.564102564102564No Hit
GTCGTATTCATCCAGATCACGGTGAACAACACCCGCACCGTCGAACAGAA12.564102564102564No Hit
GGTTTGTGGCCGTTTGCGCAAGGCAGCTGGTTGAGCATTTGGCAGGAGCA12.564102564102564No Hit
GGCTGCGCTACGCCCCGAAGCCTGAAAGTATAACAGAGGCGTGGCGCCGA12.564102564102564No Hit
GACAAGAGAACACCGAGGAAAAACTTGTAGTTGACACAGCAATAAAAAAA12.564102564102564No Hit
GTGGTCGGGATCGGCGGCACTTCGGCCTGCAGGCTGCTCGCCGTGGCGTT12.564102564102564No Hit
CTTCTTGTTGCTCACTGTGATTGTCAATGGGCAGGATGAATCGCCCCGGG12.564102564102564No Hit
GTACCAATAGAGGATGGCGGCGGCCAGCGCCGTCGCCACGGCGGCACAGA12.564102564102564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)