FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTT42.5806451612903225No Hit
TGTGGAGGTGGCCCGGGCCGCCTTCGCCGACCTGACCGGCCTGCGCCCGG31.935483870967742No Hit
TTACCAAGCATTAACTGGCCAGTCCTGACCAATACAGCTATGGTCATAAC31.935483870967742No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT31.935483870967742No Hit
GTAGTGCAGTGGCGTGATCTTGGCCCACTGTAAGCTCCACCTCCCGGGTT31.935483870967742No Hit
GTGCCCAGTGACTTATGGCCAATGTTTGATGCTTAACCCCCCCAATTTCT21.2903225806451613No Hit
GAACGTGGAGGCGCCCGGCAAGTTCGAGGTGAGCGGCGCGCAGGCATTGC21.2903225806451613No Hit
GCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTGG21.2903225806451613No Hit
TTTTTGGGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGG21.2903225806451613No Hit
CATGGGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGCCAGAG21.2903225806451613No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAA21.2903225806451613No Hit
GCAGAGTACATGGGCCTCTTCCGCTTCTGTGGTCTGATCTTCCTGCGGCT21.2903225806451613No Hit
CTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCC21.2903225806451613No Hit
AGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACACCCTCC21.2903225806451613No Hit
GTGGACACCCTCCCCCAAAAAGCCCAGAGAAAGCATCATTAAGCTCATGG21.2903225806451613No Hit
GAGAAGAGCTGGGGGCAGTGAATGGCTGAGGCTTTCTTGGGGAGCTGGGC21.2903225806451613No Hit
ATTCCAGTCTGAAACCAAAAACCTTCTGTTCAATGACAACACTGAGTGTC21.2903225806451613No Hit
GGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATC21.2903225806451613No Hit
GATTCCATTAGATGATGACCCCTTTCATTTCCATTCAATGAGGATTCCAT21.2903225806451613No Hit
GTGTGCTGGCCTCGGACACGAAGGCCCCAGAAGTGACGCAGCCCTCTATG21.2903225806451613No Hit
ACGCAGAGTACATGGGGAAAATGAACAAAAGGGAACAACAGTTTTTTCCA21.2903225806451613No Hit
GACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCA21.2903225806451613No Hit
GCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATG21.2903225806451613No Hit
GGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTGGATCC10.6451612903225806No Hit
CACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGAT10.6451612903225806No Hit
GCTGAGACTATAGGCACGCACCACCACACCTGGCTAATTTTTGTGGTATT10.6451612903225806No Hit
TCATTATGTTGGTCAGGCTGGTCTCAAACTCCTGCCTCAAGTGATCTGCC10.6451612903225806No Hit
TGATGGTAGAGCTCCTCAATCCAGGCCTGAGAGAAGACCATCAGCACGCC10.6451612903225806No Hit
TCATAGTGAAGACCTAGGAAAGGACGTTGAATTTGAAGTTGTTGGTGATG10.6451612903225806No Hit
CCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT10.6451612903225806No Hit
TCTCTGGATATTCTCTCTGGCATCGCTGATCGCTTCTGCAGCCCAGACAC10.6451612903225806No Hit
CGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCACTTATTCTACACCTC10.6451612903225806No Hit
GCATCATGATGAGTATGAAGATGAAATTCGCATGATGTCTACCGGCTCCA10.6451612903225806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.6451612903225806No Hit
TCATGAAGATCCACCTCTTAAAAGGCAGAGGACGGCTGAGGCCTTTTCAG10.6451612903225806No Hit
AGGCCGAGCTGGGGAAAGTTAATGTTCACCTGGGGGCAGGAACCCTCCTT10.6451612903225806No Hit
GGTTAGTTTTACCCTACTGATGATGTGTTGTTGCTATGGTAATCCTGCTC10.6451612903225806No Hit
ATGCTTAACCCCCCCAATTTCTGTGAGATGGATGGCCAGTGCAAGCGTGA10.6451612903225806No Hit
AGAATGTGGTGGGCGACAGCAGCGGGAAGACGATGGTCCAGAAGCGTCGG10.6451612903225806No Hit
GGCTGGGTCCAGCAGAAAAGAGGCTGAATCTTCTCCATTTGTTGAGCGAC10.6451612903225806No Hit
GAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAG10.6451612903225806No Hit
GAAGGGAAGTAACGTCAGCCTGAGAACTGAGTAGCTGTACTGTGTGGCGC10.6451612903225806No Hit
GCCCCTCTGTTGGAGGCACAGTTTTAGAATAAGGAGGAGGAGGGAGAAGA10.6451612903225806No Hit
TCATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATG10.6451612903225806No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG10.6451612903225806No Hit
CCCTTAATTCCATCCACCCTCCTCTCCCTAGGAGGCCTGCCCCCGCTAAC10.6451612903225806No Hit
TCATTAAGCTCATGGAAAAAATAGCCCAAAGCTCACTGTGTAATTAGCAT10.6451612903225806No Hit
GCATTTATTCCGTCCCAGTGGGGAGGGGCAGGGATCCAGGTGGTCGGAGG10.6451612903225806No Hit
CTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCC10.6451612903225806No Hit
ATGATAAGCATGATGATGTCATGGCCAAGTTTGGCGCTATTCTGGCCCAG10.6451612903225806No Hit
GGATGGCGAGGACCAAACTCAGGACACTGAGCTTGTGGAGACCAGACCAG10.6451612903225806No Hit
CCCTCCCCCAAAAGCCCAGAGAAAGCATCATTAAGCTCATGGAAAAAATA10.6451612903225806No Hit
GTCTTAGAGGTGGGATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACC10.6451612903225806No Hit
GCGTGTCATCGAAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTG10.6451612903225806No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT10.6451612903225806No Hit
GTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGATC10.6451612903225806No Hit
CTTACTTGCCTGTACAATATAGAGACATATGAATAGTCATAACAGTTTTC10.6451612903225806No Hit
AAGACATAACTTCCCGCCAAATTAGGGAAGCTACAGAGGAGATTGTCATT10.6451612903225806No Hit
CCAAAGCTCACTGTGTAATTAGCATTTAGAAGCTGAAGATCCCCAACTGC10.6451612903225806No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG10.6451612903225806No Hit
GAAGGAAGCGCTGGAAGACCCGGCACGCCTGTTGTTCGACATCTGATCCC10.6451612903225806No Hit
TCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGC10.6451612903225806No Hit
CTCCTGGGCCCAGGCACGCCACGGCGATACGGGCCTGTACGCCGGGCTCC10.6451612903225806No Hit
ACCCGGGCCACGCAACGATGGAGATCCGCATGCTGCTGGCGGTGCGCGAC10.6451612903225806No Hit
CTTCAAAGCTGGAGTCTGTCCTCCTAAGAAATCTGCCCAGTGCCTTAGAT10.6451612903225806No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA10.6451612903225806No Hit
TTCTCCGATAACGCAGCAGCCTGTGCAAGCGAAGCTGTAACATGATTCGG10.6451612903225806No Hit
GGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATGTCTTGAT10.6451612903225806No Hit
TCATAGTACTGAAAGTGATCTACAGATTCAATGTAATCCCTATCAAAATT10.6451612903225806No Hit
GTGCAGGAGCTGCGCAGCTCGCTGATGGAGGCGGAAAAGCTGATCGACCA10.6451612903225806No Hit
GGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAA10.6451612903225806No Hit
GGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCT10.6451612903225806No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT10.6451612903225806No Hit
CCACAGATCATATTAAGGAGTTTGTAGGTGAAGTTTAATCTGTGCATAGT10.6451612903225806No Hit
GGTACATGTCCAGGGGCCCAGCCTCTGGGCCCAGTAACTCAGCTACTCTT10.6451612903225806No Hit
GGCCTTCTTGGCTGATCCATCTGCCTTTGTGGCTGCTGCCCCTGTGGCTG10.6451612903225806No Hit
TCCCAAGGCGGGACGGTCACTTGGTTCGGTGGCGGCATGGACCTCACGCC10.6451612903225806No Hit
GCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACACCCTCC10.6451612903225806No Hit
GTTCAAAATATACTTATTAAAGGGAACAGCACCTAGAAATAGGCAGTAGG10.6451612903225806No Hit
CCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGC10.6451612903225806No Hit
CCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTC10.6451612903225806No Hit
ATCCCAAAGTTTAGAACTGGATCACTTGGCCCTTTCTCTTCTTATCTCCT10.6451612903225806No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC10.6451612903225806No Hit
GACCTGGTGCAGGGCCAGTGGGGAGGCAGCGGCACCAGACTCACCAGGCC10.6451612903225806No Hit
CTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCT10.6451612903225806No Hit
ACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTTCTCTG10.6451612903225806No Hit
GTCACTCACATGGACCCAAGATAAAAGAATGGCCAAACCCTCACAACCCC10.6451612903225806No Hit
ATCCCGTTTCGTGCAAGAATAGGAGGTGGAGTGCTGCTAGGGCTGCAATA10.6451612903225806No Hit
GAATATATGAATTTGGTATTATTATGGCCAAAGCAAAATGAGACTATCAA10.6451612903225806No Hit
AGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTG10.6451612903225806No Hit
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC10.6451612903225806No Hit
GTATCAACGCAGAGTACATGGGCGGCATTATCCTCCTGCTTGCAACTATA10.6451612903225806No Hit
CCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTC10.6451612903225806No Hit
CTTGTAACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGC10.6451612903225806No Hit
AGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT10.6451612903225806No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT10.6451612903225806No Hit
CCTCGACAGCCTGCGCGAGGAGCTGCCCTCGGCCATCCTGCTCGACCTGA10.6451612903225806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTAGAGATGCTTGTGGTTT10.6451612903225806No Hit
CTGTCACTATTCTCAGGTGGGTTTTTGAGAATGAATGTGCAGAGTTTATG10.6451612903225806No Hit
GACCATGTCCTCTCAAGGTCGAGGAAGAGGCCGAGGGCGTGGAGGAAGAG10.6451612903225806No Hit
CCGTCGTCGTCTCCCTTCAGTCGCCATCATGATTATCTACCGGGACCTCA10.6451612903225806No Hit
GTATTTCCCAGCACATGAAACCTTATTTTTTCCCAAAGCCAGAACCAGAT10.6451612903225806No Hit
GCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAGGG10.6451612903225806No Hit
GCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACAC10.6451612903225806No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACAC10.6451612903225806No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG10.6451612903225806No Hit
ACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTG10.6451612903225806No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA10.6451612903225806No Hit
GGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCCTCATG10.6451612903225806No Hit
CCTCATTCTCCTCTAGTTTCTTGGGTTCGGTGGGGACTTGGCTCCCAGAC10.6451612903225806No Hit
AGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACA10.6451612903225806No Hit
TCGAAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAA10.6451612903225806No Hit
GTCGAAGGTGCTGCCGGGTCACGCCCTGCTGTGGCGGCGCGAGGACACGG10.6451612903225806No Hit
GTGTGCCAAGCCTTTCCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCT10.6451612903225806No Hit
CTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCA10.6451612903225806No Hit
AGCTTCTTTCTTTTTTAAAAATACAAAAATTAGCCAGGCATGGTGGCACA10.6451612903225806No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT10.6451612903225806No Hit
CTTTTGGGGGAGGGTGTCCACCAGCTTCTTCAGCTGAGCCCCTGCCTCCC10.6451612903225806No Hit
AGTTGAACCTGTGCCTGGAGCGGCTGAGTTCTGGCAAAGACAAGAATAAA10.6451612903225806No Hit
CAGATACAAGCTTCAAGGACAATTTCTTTTCGAAGGCTTATTCCAGTTTC10.6451612903225806No Hit
GCCGCCCGATGGACCGCAAGGGGCCTCTCGAGGCCGTGCACAACGAACCG10.6451612903225806No Hit
ACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTT10.6451612903225806No Hit
GCTCTAAGCTACTCAACAGCAGGGACTTTATCTTTCTACAGGACTTAAAT10.6451612903225806No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGC10.6451612903225806No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG10.6451612903225806No Hit
GGTATCAACGCAGAGTACATGGGAACGAGAATGTCTACACAGAAGATGGC10.6451612903225806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)