FastQCFastQC Report
Tue 10 May 2022
EGAF00003631163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758244
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA619638.171907723635135Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT310694.0974936827723Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG234513.092803899536297Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG206642.7252441166695682Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG132861.752206413766545Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC106171.4002089037301977Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT102471.351411946550187Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC100741.3285960719768306Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT94881.2513122424971381Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC81111.069708431586666Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG57120.7533195119249212Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT56720.748044165202758Clontech Universal Primer Mix Long (100% over 23bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG54960.7248326396252394Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG50240.6625835483037122Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA49770.6563850159051704Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG48560.6404270920706263Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG44640.5887286941934258Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC40490.5339969719509815Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC39770.5245013478510875Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC38880.5127637013942741Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA34920.4605377688448573Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA33920.44734940203944906No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC32940.43442480257014887Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC32160.4241378764619305Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC31980.421763970436957Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG29130.38417712504154333Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27390.36122936680013296No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA26810.3535801140529961Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC26000.3428975369406154Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC25080.3307642394796398Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA23310.30742083023406713Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22940.30254113451606607Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT22450.296078834781416Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17930.23646741682097056Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA17110.22565295604053576No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA16410.21642109927674993Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA15700.20705735884491006Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15330.20217766312690902No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA14700.1938689920395018Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT14670.19347334103533956Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC14020.18490090261182418Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA13950.1839777169354456Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.16340386471900867No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC12200.16089807502598108Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG11180.14744594088446464Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG11030.14546768586365338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10930.14414884918311255Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10790.1423024778303554Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10740.141643059490085Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10050.13254308639435328Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10000.13188366805408286Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA9950.13122424971381244No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT9890.13043294770548794Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA9350.12331122963056747Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9300.12265181129029706No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA9210.12146485827781031Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA8790.11592574421953883Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT8570.113024303522349Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT8440.11130981583764593Clontech SMART CDS Primer II A (100% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG8190.10801272413629384Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT8180.10788084046823977Clontech SMART CDS Primer II A (100% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA8030.10590258544742853Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7930.10458374876688771No Hit
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA7720.10181419173775197No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)