FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGACCTTGCTCCAGGTCAGCACCATGGGCGTCATCAGGTCCTAGTGC34.285714285714286No Hit
TCCACGGGGGCAGAATCCCAGCCTTCCCCACACGGTGGACCTGCTTTCGC34.285714285714286No Hit
GTTTATATGATTCAATCCAGTCATCCACCACGGACTGTGGAAAAAAAATA22.857142857142857No Hit
GGCATAGTGGCATGATCTCGGCTCACTGCAACCTCTGCCTCCCGGGTTCA22.857142857142857No Hit
CACTACTTCTGCAGAGCAGCCATAGTACGGTCGTGAATCTCTTCTACCGT22.857142857142857No Hit
GGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT22.857142857142857Clontech SMART CDS Primer II A (100% over 26bp)
CTTCTGTTGCAGCTCGACGATCTTCTCCTCCACCGTGTCGCTGGCGACCA22.857142857142857No Hit
CTTCATCGATAATCAGTAACATCAAGTTCTTGGACAGGAACATGACCGCT22.857142857142857No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG22.857142857142857No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAGTTGGAACATCTCGTA22.857142857142857No Hit
GAACTAGATGACATCCCATTCACAGGTGGCTATGTCGGTTTCCTGGGCTA22.857142857142857No Hit
CCCCCGAGTACCAGCAGATGCTTGGCGAGAACGGCCTTGGGCCGGCGAAC11.4285714285714286No Hit
GACAGGTACAGTGGCTAATTCTACCAGTGCAAAGAATTCACAGCTTAATG11.4285714285714286No Hit
GTGGCGGTGCCGGCGAAGATCACGTCTTCCATGCCGCCGCCGCGCATCGA11.4285714285714286No Hit
AATGTTTTCGAAGAAAATTAGAGAACCTTTCTAAAAGGAATGTTAGAAAA11.4285714285714286No Hit
GTGGGGGTGTACGAGTCGGCCAAGTTGATGAATGTGGACCCAGACAGCGT11.4285714285714286No Hit
CTGTGAGGATATTCGTCGACACCTCGGGATCCAGAAGTGGGCGGCTCTGG11.4285714285714286No Hit
GTCTGAAGCCCAAAGCCTGAGGACAGAAAGACCCTGATGCAGGTCAGCCC11.4285714285714286No Hit
TCGCTATTCGTAACCGCGGTCTGCACTCTGCATTCACTGTTCGTAAAATT11.4285714285714286No Hit
CCACCTCAACCGGTTGGGAAGAGGCCTCCACCTCAGCTTCCTGACATACG11.4285714285714286No Hit
GTACATGGGACTGCAAGGCGGCGGCAGGAGAGGTTGTGGTGCTAGTTTCT11.4285714285714286No Hit
CTGCTGCGGAGCCCCCGCCTGCCCGCCCGGGAGCTGTCGGCCCCGGCCCG11.4285714285714286No Hit
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA11.4285714285714286No Hit
GAGTACATGGGAAGCAGTGGTATCAATGCAGAGTACATGGAAGCAGTGGT11.4285714285714286Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAGCAGCGACGCCATGTACCTGAGTACAGGTGGCAGTTGCAGAGCCGTG11.4285714285714286No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.4285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
GTTCACTTTGTTCACGGATGTCACGCTCCGCACCGGTGACGGACTCACGC11.4285714285714286No Hit
GACTATGGCCCGATAAAAGTGAAGGATGTAATAGATCGTGGCCCTTCAAT11.4285714285714286No Hit
CGCTAAAGCCCTGCCTGGAAACAGAACGCTGTGGCCAGCCCTTCCCCCAA11.4285714285714286No Hit
GCCTGGGCCTGGCACCTGGCCGGCGACAGCGCCCGCGCGCGCAGCCTGCT11.4285714285714286No Hit
TAGACAGCCTGGTGGCCTCAACCGCTCGGTCGGTGCCGCTGTCTCTTATA11.4285714285714286No Hit
TCTATGTTTCATCTGACGACATCAAGGAGACTGGCTACACCTACATCCTT11.4285714285714286No Hit
GAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACT11.4285714285714286No Hit
TAGGTGAGCATCTACCGGTGGCCGACAGTCAGATACAAGCATGGGCGGAT11.4285714285714286No Hit
CCCTTCCACACCGACAACCCTAGCATCCAGGGCCAGTGGCACCCCTTCAC11.4285714285714286No Hit
TCTTAGAACCCCGTCAACGCAGCAGCACCTGAAGAAGGTCTATGCAAGTT11.4285714285714286No Hit
GCCAGTACGACGTCGGGCTGGACGATCCCGGACTGCCAGAAGTCGTTTCC11.4285714285714286No Hit
GTCCAAAGCCTGGGGCTGCAGTTGGTGGGGCAACTCTTCACTGTGCTTGC11.4285714285714286No Hit
AGCTGTGGCGGGAGGAGCAGCTATGGCTGCAGCAGCTACAGGAGGAGCAG11.4285714285714286No Hit
TCATATAGCAGGCTAAGCCACCTCATCTACCCTCATATAGCAGGCTATGA11.4285714285714286No Hit
GCATTAAATAATCTTTTAAAAAATGAATTATTACAGTGCACTACTAGACT11.4285714285714286No Hit
GTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTC11.4285714285714286No Hit
CTACTACAGTTTTCTTGCCCCTGTCTCACCACTGTATGTTGGATGGGGGA11.4285714285714286No Hit
GCGTAATTCTTGAGCGTACTCATCTGTGAGGCCGGTGGACCGCGCAGAGA11.4285714285714286No Hit
GGGTGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTC11.4285714285714286No Hit
GAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA11.4285714285714286No Hit
GGACGACCGTGTCCCGCAGCCGTCGTGCCCAAGCCGTGTGGACCGCGCGA11.4285714285714286No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.4285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
CTTTTGGGGATAGGCAGCTTCTTATAAAGCAGTGGGCTAAAATCATCCCA11.4285714285714286No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.4285714285714286Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTGAGACGTGCAGCGCCGCCCATTCAAAAAAGCCGGACTCATCCAGCA11.4285714285714286No Hit
CCCATGTACTCTGCGTTGATACCCCATGTACTCTGGTATCAACGCAGAGT11.4285714285714286No Hit
ATTTAAGCCTCTGGTAGTACTGAGATGAATTATATGTCTGTCTCTTATAC11.4285714285714286No Hit
TCATGCGACTGTTCCCCGGGGTCAGGGACGCTGGTATTTGCTGTAAAGAG11.4285714285714286No Hit
CACTTTTGGCAGGTATGTAAATGGAAATGTGTATCCACATGGTCGAGAGG11.4285714285714286No Hit
CTGCAAAGACGATTGGGAGGTCGAGCAACTGCGGGATGCCTGCCGCAAAT11.4285714285714286No Hit
TCGAGCACCGGCACCCGGTCCTCGGCATAGGGACCGGTGCGCACCGGCTG11.4285714285714286No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)