FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGTAGGCTCTGGGGGCCGCAAAGGACTTTAGAGATACAAGACTCAG34.285714285714286No Hit
GTTTAGCTTTTCCCAGACGTAACCGCCATCAGAGCGTGGCATGGAGCCTG34.285714285714286No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG34.285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
GCCTCAAAGCCGGCGGCACGGGGCTCAACCTCACGGCGGCCGACTACGTC22.857142857142857No Hit
CTTCAGGGGAGAGAAAGCTGCAGGGCCTAGGAGAAGAAGGCATGGAGTTG22.857142857142857No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22.857142857142857No Hit
GTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCGGTCATGGTGGT22.857142857142857No Hit
GTTAAACTCGATGCCGTCATCGAATTCCAAGTTGATGAAGATACTGTTGT22.857142857142857No Hit
GACAGGCCTCGTACGAGTCACCGCGCGTTAGCGCCTGCTGGATTGCGGCA22.857142857142857No Hit
GGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGT22.857142857142857No Hit
TTAAAAATAAGGGAGTCTCTACTTTTATTAGGCTACAGAGAAATAATTTC22.857142857142857No Hit
AGAAAGACCAGTCCTTGCTGAAAGACAAGTCTGAATGCTCCACTTTTTCA11.4285714285714286No Hit
ACATATAATTCATCTCAGTACTACCAGAGGCTTAAATCTGTCTCTTATAC11.4285714285714286No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC11.4285714285714286No Hit
CCACAGGCATCCCTAGGTTGCTGCAGTGGCCACCGCACCAACACAACAGT11.4285714285714286No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.4285714285714286No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC11.4285714285714286No Hit
AGGCCACACTCAGCACATCCTTGGTATGGCCCACAAATCGCCTCGTGGTG11.4285714285714286No Hit
ACTTTGAGCGTTTAATCTTCAAAATTTTAGGACATCATCAATTATACCTT11.4285714285714286No Hit
GTAGCAGGCTGCGTAGATATCGTCAGCGGATCGCCCGACCGCCGACAGAC11.4285714285714286No Hit
GTGGTGGAGCTGCTCTGGCCCTGGTGGAATTGAAAGGACTTATGTAGATA11.4285714285714286No Hit
CCTCTGGTCAGCCCGTCAATGCGCCCGCCTTCACCGCCCCCATGCGTTTC11.4285714285714286No Hit
GTACTATGACTATCTCCATGTCCATACAATCCGCTTACTGTACTTCCACT11.4285714285714286No Hit
TTTCAGATGTGTTTTATCCGGGAGGCATTATGTTACTGGCAGGCGCTATC11.4285714285714286No Hit
GGGCGAGGACGGTGGCCGCTGGCTGGCGGTCTGTGGCGACGCTCATGGCG11.4285714285714286No Hit
GGAGTAAAAATTAGCACCGAGCGAACCCAAGGATGGGAACTCACCTGTTA11.4285714285714286No Hit
AATTAAGAGATACTGAAAATATAGCTCACCCCTCAAAATTAGACAAATCC11.4285714285714286No Hit
CCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACA11.4285714285714286No Hit
CTCCATATACTCTGGCAAGGGCATCTTTAAAATCTGCAACTTGGTAGTAA11.4285714285714286No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11.4285714285714286Clontech SMART CDS Primer II A (96% over 26bp)
CTTCCTCATCTCAGGTTTTAGGGATGCCACTTGCATAAAAATGAGTGGTT11.4285714285714286No Hit
CGGCACCGACCGAGCGGTTGAGGCCACCAGGCTGTCTACTGTCTCTTATA11.4285714285714286No Hit
TTGTGAATGTGTACAGAGAGATAGGGGTAGGAAACGTGAACTGCCCTCGG11.4285714285714286No Hit
GCTGCTGGGAAGGGCCTCAGCGTTCCTGAAGAGAGATGTAGGGGACCCAC11.4285714285714286No Hit
ACGGATGTCGTTTGCACTGGAATGGGAGACGGTGATGACGTGGTCGCACA11.4285714285714286No Hit
CATCTAAGGTGACTGCACAGCAGGTGGGAAACAGCTGGTGTGGTCTGCTG11.4285714285714286No Hit
CTTTCTGTCTGTGTCAGATATTATTGACTACTTCAGGAAAATGCCACTTC11.4285714285714286No Hit
GGCGCGGACGACAACGCCTGTCGAGTCGATGCGGGAGGCAAGGATGCGAG11.4285714285714286No Hit
CTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATA11.4285714285714286No Hit
TCACAATGCAGCGGATCTCCTTCACCTGGGGGTTATCTGGTGGGCTCACC11.4285714285714286No Hit
ACTTCGCCCGACGGAATCCAGGGGTCGTAATATATGTAAACTCGCGTCCG11.4285714285714286No Hit
GCCTTCGACGACCGCGCGACTCCGGCCGAACTGGGCCAGAGCGCCCGGGT11.4285714285714286No Hit
CTGCAGCGCAGTGGCGCGGGCCAGATACCAGCGCGTCCGCAGTGCCGGCA11.4285714285714286No Hit
TTCAAGTACGGTTCCAAGGCCGACTTCTGGCTCAACGGCAACTACATGTC11.4285714285714286No Hit
CTTTAGTTCCTCTACTGAATTTCGTTTGGCATCAGAAGATTTAAAGATTA11.4285714285714286No Hit
GCACGGATCGCGTCAGAACGCGTGGTCAATCCTTCGTCAGGGGCTCGTTG11.4285714285714286No Hit
GACTTCGATGTCGATGCCGCCATGGCGGCCGTCATCCCCGTCTGCTCTGC11.4285714285714286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT11.4285714285714286No Hit
AGATTCTACTTTTTGATTGGAGAATGTCAAGTTCCTATAGCGAAGTGGCA11.4285714285714286No Hit
CGATCCCAGCGTCGATAAGGATAAGTTCGCCGGGGCGCAGGTCGCCGTCG11.4285714285714286No Hit
ATCCTCGGCGGTGACACCGCCGCGGCGGCAACAAGTGCAGACGTGATGCA11.4285714285714286No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.4285714285714286Clontech Universal Primer Mix Long (95% over 21bp)
TTTCTCACCCCTCTCGACCATGTGGATACACATTTCCATTTACATACCTG11.4285714285714286No Hit
GCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTGGCGCAAG11.4285714285714286No Hit
CTCCAGAAGGAAGGGGGTCGCCCTGCTAGTGACGGAGCTGGGAGTCACCC11.4285714285714286No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAA11.4285714285714286Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)