FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA45.128205128205128Clontech Universal Primer Mix Long (96% over 26bp)
GGCGCTGGCACGGCGTGCCGTTCTACCTTCGCCATGGCAAGCGCCTGCCC33.8461538461538463No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG22.564102564102564Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT22.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
GAAGTCGTTGAGCGCGAGGAGGCGCGGCAGGAGAAGCTTGCCGAGATCGA22.564102564102564No Hit
CAGTTATATATCCTTTCAAAGATTCGACATCTTTAAGATATTCCGGGTCA22.564102564102564No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22.564102564102564Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACCCGGGCGGCGGTGACGGCGGCGCTGGAGGCGGGGTATCGCCACATCG22.564102564102564No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG22.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGTCGGTCGGCGGGCTCACGGGCCGCGGGATGGATGCGGTCGCCG22.564102564102564No Hit
TAACAAAACTGTACATTCTGCACATGTAACCCAGAACTTAAAGTAAGATA22.564102564102564No Hit
GAATGATCACGCCAATTTGGGTCCCAGTCGTTCCTCGATCGTTGCCACAA22.564102564102564No Hit
CAGTAAAGGCATATTAGGAAAATGCAAGGAAAAAGAAAACAGCGGGAATC22.564102564102564No Hit
GCATGTTAGTGTCATTTATGTTGATTTTAGTTATTTTTTTCAACGATTTA22.564102564102564No Hit
GGTCATTTTTGCTGTCACCAGCAACGTTGCCACGACGAACATCCTTGACA11.282051282051282No Hit
GCTGATGCACTGAATCTCAATCAGGAAGCTCTTAATGCATGGTACAACTG11.282051282051282No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGG11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
ATTTATTAACAGACAAGGCCTACAGACTTATTTCTTCTTGGACACACCCA11.282051282051282No Hit
GCTCGGGCACCGAGCGTGGTGGCTGCCGGGCTGGCTCGACCGCTCCCTGC11.282051282051282No Hit
ACCTACGCGGCCATCACCTCGATGCAGTCGGGGGGGGGGGGGGTGGGGGG11.282051282051282No Hit
CTCTATGCTGACGTCGGTGGAAAACAATTCCCTGTCACTCGAGGCCAGGA11.282051282051282No Hit
GTGCCGGCGTCATCAAGACCACCTTCACCGAAGAGACCGAGACCGACCTG11.282051282051282No Hit
ATGGGAAGTAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11.282051282051282Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TTCCCATGTACTCGGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTC11.282051282051282Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
GGATTATGCGTTCATTTATACTTCTGCCGGGCGGCAAATTACTGTGAATG11.282051282051282No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTGCGTTGATAACACTTCTTCCCAT11.282051282051282No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTGCGTAGATACCACTTCCCCATGT11.282051282051282No Hit
GGAGCGGTCCTGGCTCTTGTAGTTCCACGTCGTCACCGGCACGCCGCGAA11.282051282051282No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
GAACGAGCCAGATACCTGGAGAACTATGATGCCATCCGAGTTACTCATGA11.282051282051282No Hit
CGGTGACGGCGCCGGCCAGGTCGGTCAGGATGTCGCCGAAGAGGTTGTCG11.282051282051282No Hit
GCTTGCAGGAGCCCATTTCCCTCCGGCTCGACGTCGACATAGAGCAGGCA11.282051282051282No Hit
GTTGATACCACTGCTTAACATGTACTCTGCGTTGATACCACTGCTTCCCC11.282051282051282Clontech Universal Primer Mix Long (96% over 27bp)
CAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
ATGTCGTAGCGCTCGGCAACACCTTGGTGGTCCAGGCTTGCTAGCGCGAT11.282051282051282No Hit
CCCACGTCTTAGAACCTTCACCACAAGGAGTTTTTCTCGTAGTGATTCTC11.282051282051282No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACAT11.282051282051282No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG11.282051282051282No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG11.282051282051282No Hit
GGTTGCCACACCTATACACTTCCCGTTCTTGAAAAAGGTGAGTGTGCCGT11.282051282051282No Hit
GGACTGGGCCATTCTCCTTAGAGAGAAGTGGGGTGGCTTTTAGGATGGCA11.282051282051282No Hit
CTCCTGCTGCCTCTTCTGAAGAACCTCCGTTTGCACCTTCTTCCCGTATT11.282051282051282No Hit
AATTAACAGTCTTTATTGGGCTCAGACCAGGAGTCCGTGGGTCTTGAGGA11.282051282051282No Hit
CATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACT11.282051282051282No Hit
CAAAAACACATCTCCGAGCCCACGAGACTTGGACTCCCACATCGCGGATG11.282051282051282No Hit
GTGCAGTGGTGCAATCTAGTCCTGCTGCAACCTCTGCCTTCCAGATTCAA11.282051282051282No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA11.282051282051282Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCAGGGACTGTGAGGTTCAAAAGAAAGAACAGAAGTTAAAATGCTTTGA11.282051282051282No Hit
GTCCACTCGGGTACGAATTTCACCCATAAGGTCGTCAATCTGGCCATGCG11.282051282051282No Hit
GGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAC11.282051282051282No Hit
AAGTAGCTCCGAACAGGAAGAGGACGAAAAAAATAACCGTCCGCGACGCC11.282051282051282No Hit
CTCCGATTTCGCTCGCCACTACTTTCGGAATCTCGGTTGATGTCTTTTCC11.282051282051282No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
CGGCTTCACCGAAGACGCGGCCGTGATCGCGGCCCTGGTGGCGACGCTGG11.282051282051282No Hit
GTGGTTAGCTCCTTCTTGCCAACCAACCATGAGCTCCCAGATTCGTCAGA11.282051282051282No Hit
CCTGCTGCCTTCCTTGGATGTGGTCGCCGTTTCTCAGGCTCCCTCTCCGG11.282051282051282No Hit
GCTCTATAAGCATGTGAACGTTCAGTATGCTCCATTTTCATTTCAAAGAA11.282051282051282No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)