FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45.128205128205128Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC33.8461538461538463Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT33.8461538461538463Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG33.8461538461538463Clontech Universal Primer Mix Long (96% over 26bp)
ATCATCGTCAGCTGGATGTTCTCTTCGGGCTGGATGCCGATGACCAGCCG33.8461538461538463No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG22.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
CTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAAC22.564102564102564No Hit
CCCAATAGGCAGATCAATGATGGGATGGGTGGAGATGGAGATGATATGGA22.564102564102564No Hit
GGCCAGGTGACGACCGCTCACACCGACTTCTTGCTGGCTCGCAAGCTGGG22.564102564102564No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG22.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
TTGCAGCAGTAACGTAGAAGACAAAGAATGGCCAGTAGTTCGGCCAGAAT22.564102564102564No Hit
GTGTTAATCCAAAGATCAAGGATACCAGATCTGTTCATTTCTTGTCTTAT22.564102564102564No Hit
ACCATGAAGTTGCTGACCCCGATCGCCCGCACCTTCCCCTGGCCGAGCAG22.564102564102564No Hit
GTGCGTGCGGCACGAGGAGGCCGAGGAACACGATCGGACCCGCGATGGCG22.564102564102564No Hit
ATTGTGTCGAGGCGTTCAAGCACGGCCTTCCGCTCCATGGGCGTGCGGAC22.564102564102564No Hit
GAAAGAAAAGGCACCTGCTTCTCTGATCTGTGAAGATCTGTGGGCTGTAT22.564102564102564No Hit
CGTCTACAAGGGCAATCTCGCCGCGATGATCAACTATTACAGCGGCCCGG11.282051282051282No Hit
CGTCGATGGTGGGCGTGGAAGGACCGGCGGTCAGACCAGGGCCGGGTCTC11.282051282051282No Hit
GTCGAGGCCATCTCCTGGGCCGCCTGGCGGCCATCGTGGCTAAACAGGTA11.282051282051282No Hit
CGCCAGAGGTGTGTCGTCCATGCCGATGAGCCCGAAGTCCTCGGGCACGT11.282051282051282No Hit
TGCCTTGGCAATGATAGGCGACGAAGGACGTGATAGCCTGCGATAAGCTT11.282051282051282No Hit
GTAAAATCCTTGGAAAAGGTGTGTGCTGACTTGATAAGAGGGCAAAAGAA11.282051282051282No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCGAGTACATGGGAAC11.282051282051282Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTAT11.282051282051282Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.282051282051282No Hit
GAACAGGCCCAGCGGCTGGCCATCGGCACCTTCGTTGGCGCCTCCTCGCT11.282051282051282No Hit
CCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTC11.282051282051282No Hit
CCCCCAAAGTTCACAATGTGGCCGAGGACTTTGATTGCACATTGTTGTTT11.282051282051282No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.282051282051282Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCT11.282051282051282Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAACAGGCTGCCATGGAGAGGTCTGAAAAAGCTAAGCAACTGCGAGCACT11.282051282051282No Hit
GGGGGGATTTTGCCATTGAAAAATAACCTGGAAATAAGTCAAGACTGATC11.282051282051282No Hit
TTCTTGAACAGACAGAAGGATGTAAAGGATGGAAAATACAGCCAGGTCCT11.282051282051282No Hit
TTCCAAAGCTGTGTGCTATCAGCAAGGTTCTGTTGAAGGACACTGGATTT11.282051282051282No Hit
TAAGGATGGCAATGCCAGTGGAACCACGCTGCTTGAGGCTCTGGACTGCA11.282051282051282No Hit
CAGACTGGGCCATGGGAAGATACAGAGAGGCTCCAGGAAATGCGGCTGAC11.282051282051282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTCA11.282051282051282No Hit
GCGTATGCGGCAGTTCCAAAAGCAATAAGTGCAATAATTACAAATAATTT11.282051282051282No Hit
GGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11.282051282051282Clontech SMART CDS Primer II A (100% over 26bp)
GGCTACTGACTGTGTGTGGTGGTGGTCTGGAACCTGGACCTGGAAGCTTT11.282051282051282No Hit
GAACATGGTTTCCGACTGCCTAGCGCGATGGACAACCGTCCTCTCAAGTT11.282051282051282No Hit
GGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGA11.282051282051282No Hit
GAAGAAGCTCACGCTCGTCCACAAGACCAACGTGCTCGTGCACGCGGGCG11.282051282051282No Hit
CCCAACAACAAGGCCATGGCAGACGAGCTGAGCGAGAAGCAAGTGTACGA11.282051282051282No Hit
GGTATCAACGCAGAGTACATGGGCCACAAGGGCGACAAGACCAGCTTCTC11.282051282051282No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.282051282051282Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACC11.282051282051282No Hit
GCTTGAGAGTGAGCCTTTCGAAGAGATACTCGCCCAGCCCAGCCTCCGGG11.282051282051282No Hit
GTGTGGAAGTGGCGGCGGGCGGCGGCTCGTGGAGCAGCATCTCGGACCGC11.282051282051282No Hit
GGGCTGGGGTGCCGCCCTCAGGCCTGGATGTGGCTCTTCGCACTGAAGGC11.282051282051282No Hit
ATTATTGACAAGCTATGCTGCAGAAAGAGCAATCGCATTAAATCTTAGTT11.282051282051282No Hit
CCTTGACGCGCGGGTCGATGACGCGCGGGCCCGAGACGTAGTCGCCGTAC11.282051282051282No Hit
GTAGTTGCGCTCGCGCCGGGCGCCGGCGCCGCCGTGCAGGATCAGGGCGG11.282051282051282No Hit
AACGAAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAC11.282051282051282No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)