FastQCFastQC Report
Tue 10 May 2022
EGAF00003631305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33277
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCTCGTATG15194.564714367280705No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCGCGTATG12663.804429485831055No Hit
ATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCTCGTATGCCG7962.392042551912733No Hit
ATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCGCGTATGCCG7642.2958800372629744No Hit
ACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCGCGTATGCCGTC5231.5716560988069839No Hit
ACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCTCGTATGCCGTC4901.4724885055744208No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4001.2020314331219761No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3891.1689755687111218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.9315743606695315No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA2700.8113712173573339No Hit
CATCTCCGAGCCCACGAGACTCGGCACCCAAGATCTCGTATGCCGTCTTC2580.7753102743636746RNA PCR Primer, Index 6 (95% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2250.6761426811311115Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA2010.604020795143793No Hit
CATCTCCGAGCCCACGAGACTCGGCACCCAAGATCGCGTATGCCGTCTTC1990.5980106379781832No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1660.4988430447456201Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.4928328875800102No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA1440.43273131592391145No Hit
CACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCGCGTATGCCGTCT1380.41470084442708177No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA1370.4116957658442768No Hit
CACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCTCGTATGCCGTCT1260.3786399014334225TruSeq Adapter, Index 6 (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA1250.37563482285061756Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1190.3576043513537879Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1170.35159419418817806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1080.3245484869429336No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1070.3215434083601286No Hit
TCTCCGAGCCCACGAGACTCGGCACCCAAGATCTCGTATGCCGTCTTCTG1040.3125281726117138Illumina PCR Primer Index 1 (95% over 24bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT990.2975027796976891Clontech SMART CDS Primer II A (100% over 26bp)
TCTCCGAGCCCACGAGACTCGGCACCCAAGATCGCGTATGCCGTCTTCTG960.2884875439492743RNA PCR Primer, Index 28 (95% over 23bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA900.27045707245244466No Hit
TTATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCTCGTATGC870.2614418367040298No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.24942152237281004No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA830.24942152237281004No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA760.22838597229317545No Hit
TTATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCGCGTATGC750.22538089371037054No Hit
TATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCTCGTATGCC730.21937073654476064No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT720.21636565796195573Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT710.21336057937915076Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAA700.21035550079634585No Hit
TATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCGCGTATGCC690.20735042221354089No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCTGGTATG690.20735042221354089No Hit
ACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCGCGTATGCCGTCTT680.20434534363073595No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA660.19833518646512607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG660.19833518646512607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT650.1953301078823211No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG650.1953301078823211Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAA640.19232502929951617No Hit
ACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCTCGTATGCCGTCTT630.18931995071671126Illumina PCR Primer Index 1 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA620.1863148721339063No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT600.18030471496829642Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA600.18030471496829642Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA590.17729963638549148Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG590.17729963638549148Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT570.1712894792198816Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT560.16828440063707664No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT520.1562640863058569Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA520.1562640863058569No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA520.1562640863058569No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAA510.15325900772305195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG500.15025392914024702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA500.15025392914024702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG490.14724885055744208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG480.14424377197463714No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAA480.14424377197463714No Hit
GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA470.14123869339183218No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCGCGGATG460.13823361480902727No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.13823361480902727No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA450.13522853622622233Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAA450.13522853622622233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT450.13522853622622233No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCCAAAAAAAAAA450.13522853622622233No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA440.13222345764341736Clontech Universal Primer Mix Long (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCGGGTATG440.13222345764341736No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA430.12921837906061243No Hit
ATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCTGGTATGCCG430.12921837906061243No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCTCGGATG420.1262133004778075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT380.11419298614658772No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.11419298614658772Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT370.11118790756378279Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA370.11118790756378279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA370.11118790756378279No Hit
CTCCGAGCCCACGAGACTCGGCACCCAAGATCTCGTATGCCGTCTTCTGC360.10818282898097786TruSeq Adapter, Index 6 (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC360.10818282898097786Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTCGGCACCCAAGATCGGGTATGCCG350.10517775039817293No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT350.10517775039817293Clontech Universal Primer Mix Long (96% over 26bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCGGCAC340.10217267181536797No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)