FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003631421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTCCAAGCCTCGGTCAGCTCGGTCTTCAGTTCACACCCTGTCTCTTA211.11111111111111No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15.555555555555555Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACACGAATCAAATCATCGTTGAATGGAATCGAATGGAATCATCGAATGG15.555555555555555No Hit
GGTCCCAGGCGTTCACCCGCTCAGGCAATAGCCCGCCGCTGGACAGATAG15.555555555555555No Hit
CTGTCGGGTCTCATTGGAAGTGATTGCTCCCGAGGGCACATCTCTCTCAG15.555555555555555No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAACACCGTGAA15.555555555555555No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTC15.555555555555555Clontech Universal Primer Mix Long (96% over 26bp)
ATAGTGAAGCTAGAAGTAAAACTAGTTCTCCTGATTCTGGAAAAAGTAGT15.555555555555555No Hit
TCCTTGTTTCCTTCCTTTCCTTCCTTCCTTCCCTCCTTCCTTCCCTCCTA15.555555555555555No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA15.555555555555555Clontech Universal Primer Mix Long (96% over 25bp)
CCGCTTCCCCAGACCCTGCCACCGGAAACAGCCCAAGAAGCAGGCCAAGG15.555555555555555No Hit
GGACATCACCATGCAACTCAAGGACAAGGTCGCCTACATCACCGGCTCGG15.555555555555555No Hit
GCATGATCTCATCATCCGCATGCACGAGAGTGGCGGCAACGACCTTGCCA15.555555555555555No Hit
GAGTAAACCTGAATCTTTGGAGTACGCTGGATAGCCTCCAGGCCAGAAAG15.555555555555555No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTTACCAAAGGTT15.555555555555555No Hit
GATGATAGGATGTTTTATGTGGTGTATGCATCGGGGTAGTCCGAGTAACG15.555555555555555No Hit
GAGTTCACCATGTTTTTTTTTTTTTAAGTATAAACTGTACAAACATGATT15.555555555555555No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)