FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003631422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTGAACTGAAGACCGAGCTGACCGAGGCTTGGAATACCTGTCTCTTA211.11111111111111No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15.555555555555555Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCA15.555555555555555Clontech Universal Primer Mix Long (96% over 26bp)
CCATTTGATCCCATTCGATGATTTCCTGTGATTCCCTTTGATGATCATTC15.555555555555555No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA15.555555555555555Clontech SMART CDS Primer II A (96% over 26bp)
CTCTTTCTGGCCTGGAGGCTATCCAGCGTACTCCAAAGATTCAGGTTTAC15.555555555555555No Hit
GTGCAGCTGTCACTAGGTTTACATCCTCCGTCTTAACATTGCCCACCCAA15.555555555555555No Hit
AGGCGGTGTTTCACTGTGTTAGCCAGGATGGTCTCGATCTCCTGGCCTCA15.555555555555555No Hit
ACACTAGGTGTGTTTGCTTCTGAACAGAGACTTGGATTTATTTACCTGAG15.555555555555555No Hit
TCTTCCAGTCGGCGAAGCTGAACTCCTCCACCGGATGCACGATCTGGATG15.555555555555555No Hit
ACCTGGGCAGCCTCATTGAGCGCGACAGTTATGCCCCAGCGCAATGGGCG15.555555555555555No Hit
GTGGTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAACTGTGA15.555555555555555No Hit
TATACGTGGCAAAAGATGTTATGGCAGGGAATAGAGAGGTTTAAATACAG15.555555555555555No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT15.555555555555555No Hit
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTATC15.555555555555555Clontech SMART CDS Primer II A (100% over 26bp)
AGAGGGAAGGAAGGAAGGAGGGAAAGAAGAAAGGAAGAAAGGAAAGAGGG15.555555555555555No Hit
GCGGCAAGGCCGTGCGCTTCGACGAGGAGGACGTCCGTCCGCTGCGCCGC15.555555555555555No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)