FastQCFastQC Report
Tue 10 May 2022
EGAF00003631433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917251
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG53800.5865352013789028No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG36170.39433045044377163No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA33400.36413151907166086No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG31870.34745124289861773No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC31070.3387295298669612No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT28010.3053689775208749No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC25020.27277157506505856No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC23010.25085827107302144No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT22960.2503131640085429No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG22620.24660643597008888No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA22280.24289970793163487No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA20800.22676453882307024No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC20000.2180428257914137No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG19850.21640750459797808No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT17340.18904312996115566No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG17270.18827998007088573No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA16660.1816296738842476No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT16010.17454328204602668No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG15990.17432523922023524No Hit
CCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGG15380.16767493303359712No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG14710.16037049836958478No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT14700.16026147695668908No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC14120.15393823500873807No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT14010.1527389994668853No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC13540.14761499306078707No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA12550.1368218731841121No Hit
CACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCC12180.13278808090697095No Hit
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT11900.12973548134589113No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA11900.12973548134589113No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC11850.1291903742814126No Hit
GTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTA11790.12853624580403838No Hit
GGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA11430.12461147493979294No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT11300.12319419657214875No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT11290.12308517515925302No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG11190.12199496103029596No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA11060.12057768266265176No Hit
GTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT11040.12035963983686036No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA10900.11883334005632046No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA10650.1161078047339278No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA10590.11545367625655355No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC10490.11436346212759649No Hit
GTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCTGTCCCAATGT10400.11338226941153512No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC10390.11327324799863941No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC10370.11305520517284799No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT10290.11218303386968236No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC10270.11196499104389093No Hit
GCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAAC10230.1115289053923081No Hit
TGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATG10210.11131086256651668No Hit
CCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG10120.11032966985045534No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG10100.11011162702466391No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTAC10060.1096755413730811No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG9990.10891239148281115No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT9930.10825826300543691No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT9860.10749511311516696No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT9830.10716804887647984No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG9740.10618685616041847No Hit
TCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCC9700.10575077050883565No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA9560.10422447072829574No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC9440.10291621377354727No Hit
CTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAG9440.10291621377354727No Hit
CTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGC9370.10215306388327733No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC9310.10149893540590307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)