FastQCFastQC Report
Tue 10 May 2022
EGAF00003631477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674713
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCT37000.22093337783847142No Hit
GAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCT32610.19471993111655547No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGG32550.19436166077411474No Hit
GGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCT28670.1711935119629453No Hit
CTCCGGGACCATCTTCTCGGCCATCTCCTGCTTCTGGGACCTGCCAGCAC25060.14963757969275931No Hit
GTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG23910.14277073146264466No Hit
GGCTACGAGCGTCTCCTGAAGATGCAAAACCAGCGTGGCGGCCGCGCTCT20600.12300615090466246No Hit
GTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCC20270.12103566402123826No Hit
TGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGG19350.11554218543714655No Hit
CTTCAGAAGTCGCTGGGCTCAGAAGGCTCTTAGTCGTGCTTGAGAGTGAG19260.1150047799234854No Hit
CTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCCAG18680.11154149994655801No Hit
TCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGGGC17880.10676456204734783No Hit
GCTCTTAGTCGTGCTTGAGAGTGAGCCTTTCGAAGAGATACTCGCCCAGC17840.1065257151523873No Hit
GCTTGAGAGTGAGCCTTTCGAAGAGATACTCGCCCAGCCCAGCCTCCGGG17520.10461493999270324No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA17470.10431638137400258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)