FastQCFastQC Report
Tue 10 May 2022
EGAF00003631478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674713
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCT35820.2138873944371364No Hit
GAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCT30250.18062796431388542No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGG30150.18003084707648415No Hit
GGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCT28370.16940216025074145No Hit
CTCCGGGACCATCTTCTCGGCCATCTCCTGCTTCTGGGACCTGCCAGCAC23780.141994479054023No Hit
GTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG22530.13453051358650708No Hit
TGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGG20210.12067739367879751No Hit
CTTCAGAAGTCGCTGGGCTCAGAAGGCTCTTAGTCGTGCTTGAGAGTGAG19530.11661699646446884No Hit
CTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCCAG19420.11596016750332744No Hit
GTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCC19330.11542276198966629No Hit
GGCTACGAGCGTCTCCTGAAGATGCAAAACCAGCGTGGCGGCCGCGCTCT19290.11518391509470578No Hit
TCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGGGC18890.11279544614510068No Hit
GCTCTTAGTCGTGCTTGAGAGTGAGCCTTTCGAAGAGATACTCGCCCAGC17360.10365955241286118No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA16820.10043511933089432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)