FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGAC25.555555555555555No Hit
GAGCAGGACGGCAAGCGAGCCTGCCCTGCGCGCCGTGCGGCGGGCCCGGC25.555555555555555No Hit
ATTGAGATCCGGGAGTCCATTGAAGCAAACAGCAATAACTAGAAAAAGTC25.555555555555555No Hit
GCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGG12.7777777777777777No Hit
CTTCTGCGCCGCGCGGATAACCAGCGCTCCCCAGCCGCAGCCGATGTCGA12.7777777777777777No Hit
TGCCATCCTGCGTCTGGACCTGGCTGGCCGGGACCTGACTGACTACCTCA12.7777777777777777No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGAGTACATG12.7777777777777777Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCTCCCTACTAATCGGGAAATGCCGGCCCAAACCAGCTCCAACCAGGCAA12.7777777777777777No Hit
CTCCTGAGGTCCACCCTGCCAGCCGTACTGTGGGTATCCTTGGTAGGGGT12.7777777777777777No Hit
GGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGCCAGAGCA12.7777777777777777No Hit
GCATCACGGCAAGCTACGACCGGTTGTTGATCGCCACGGGATCGTCGCCG12.7777777777777777No Hit
ATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTGTGCTTTTTTTA12.7777777777777777No Hit
CTCCTCATCCTCACTCTCTGGCAGCATCTGAAGGTTTTCTAGTGTCAGCT12.7777777777777777No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT12.7777777777777777No Hit
TTATCTAAAATGTTAGCGTTGAAGGCATGATGAATAAATATAACAGAACA12.7777777777777777No Hit
GTTTCCACCGCGATCTCCACGCCCATCAGTGCGCGCACGCCCACGTCGAT12.7777777777777777No Hit
GGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGG12.7777777777777777No Hit
CCGGTGTCCTGTCCGCCATGGCCGTCGGCGGTCTCGTGGTCGGGCTGCTC12.7777777777777777No Hit
CCCTTGGGAATCATGGCTTGGATCCGCTTGATCAGAGCCCTGCAGAGCCA12.7777777777777777No Hit
TTATTATGGAGGGAGATGAGATGAGGTTGGAAAAGGAAGCAGTGAGGTTG12.7777777777777777No Hit
ACCCTGCCCATCTCTAGTTCTGGAATCGACCACGCAGCCAGACAGATGTC12.7777777777777777No Hit
CATTCACGCGGATCGCGACCGGCCGCGCACCGCGCAGCGCATCCGCCAGC12.7777777777777777No Hit
CTTTCAGTTCAACCTTTTCCTCCTCTTGCTCTGACAAGTCCACGACCACT12.7777777777777777No Hit
TCATTATCTTAGCCACCAGAACACAGAATGTTCTTGGTGAGAAGGGCCGG12.7777777777777777No Hit
GCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTT12.7777777777777777No Hit
CCTCTAGAGAACCCACGGCCCCGTCCTCGGCCCTCACTCGCGCGTTAGGA12.7777777777777777No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC12.7777777777777777Clontech Universal Primer Mix Long (96% over 26bp)
TCATTATCTTAGCCACCAGAACACAGAATGTTTTTGGTGAGAAGGGCCGG12.7777777777777777No Hit
GTCCTAGACTGGCTCGCCTCCCACCTCCACCGGCGTCATCGGCCCAAAAC12.7777777777777777No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT12.7777777777777777No Hit
GGCTTTGAGTGTAGCACTTGGTAGTTCTTCCTCTGCTCTGCTTCCCTTCG12.7777777777777777No Hit
CTACTGCTGCGGCCACTGGGTTTCGGCCTCTTCCCAGCAGCGGCTCTAAG12.7777777777777777No Hit
CTCTGTGGCCCAGGCACTGCTGCCTGGACCACCTCATCCTTGGCCTGTGC12.7777777777777777No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)