FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAGCACCCAGCGCACCGCATCCATGATGTTCGACTTGCCGCAACCGTT25.555555555555555No Hit
CTGTCACACTCATCACTCATGACTGTGCCCTGAATGGTGGCATACAGCCA25.555555555555555No Hit
TACCACTCCCAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGT25.555555555555555No Hit
CAGCACACCCTGTCTGAGCAACACGTGGCGCACAGCAGTGTCAACGTAGT25.555555555555555No Hit
TCCCAGTGCCAACGAGGTCTTTTATAACCCGGTGCAGGAATTCAATCGGG12.7777777777777777No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG12.7777777777777777Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGGGTCACGGGGTCGCGACTCTCGACTTCAACCTCACTGCTTCCTTT12.7777777777777777No Hit
GCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAG12.7777777777777777No Hit
ATCACACCCGGCAGGTCCGTGCCCGGAACCGGCAGGATGAACGGCGACGA12.7777777777777777No Hit
GCCAAGGACCTGCTGCTGCGCGAGATGGCCCATCGCACCAAGAACAATCT12.7777777777777777No Hit
TGCTGAAGAAGGTCTTTTACTGCATCTCGCTCTTTCACTGCTGTGCTTTC12.7777777777777777No Hit
GCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGC12.7777777777777777No Hit
GCGGATGGCCGACCGGGCGTGGTCGATCGCGTCCGGCCACAGCTCGCGCA12.7777777777777777No Hit
GCAATCGGGTGTCTAAGCACCGGTCATGCTGCGTGAGATTGCAGCTGGGT12.7777777777777777No Hit
ACGTGTCAAGGCGTCGTCATAGGCTGTTGAACAGCATCTCTTTGTCCCCG12.7777777777777777No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA12.7777777777777777Clontech Universal Primer Mix Long (96% over 26bp)
GCTCGTGAAGCGCATGAACCGCAGCGTGCTGCTGCGGCTGCTGCGCGCGC12.7777777777777777No Hit
GCTCATGCCCCAGTGCAACCAAGTGCTTGACGACTACTTCCCCCTGGTCA12.7777777777777777No Hit
GTACATGGGAAGCAGTGGTATCAAACGCAGAGTACTTTTTTTTTTTTTTT12.7777777777777777No Hit
CAATATGCAGTTTATGAGGGTTCTGTGGATCCCCACCGTATTTTTCATGA12.7777777777777777No Hit
CTAGTAAGCCTCTACCTGCACGACAACACATAATGACCCACCAATCACAT12.7777777777777777No Hit
CAAGAAGATCGACCACATCCTCACCAAGATCGAGCTGAAGCCGGGCCAGA12.7777777777777777No Hit
CTGTCCGTACTTTGAGAAGACCTGCTCCAGCGACTGCTCATTGGTGTCAA12.7777777777777777No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGG12.7777777777777777No Hit
GCGTTAAGGATGTGGAGGCTTTCTCTACAACTCGTTGTCTCACCCGGCCG12.7777777777777777No Hit
GTGATGACCTGGCCGTCAGGCAGCTCGTAGCTCTTCTCCAGGGAGGAGCT12.7777777777777777No Hit
CCACCATATCCAGGCCCTGGTCCAACGGCCCCATACCCACCTTATCCACC12.7777777777777777No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA12.7777777777777777No Hit
TCAGAAATGGGTCGCATTGCAAGAGATCTTACACATTTCATTATCAATAG12.7777777777777777No Hit
GTGTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGG12.7777777777777777No Hit
GTCCAGCAGAGGGTCTGGCAGAGGGTCCCGCAGAGGTTTGGGCAGGGGGT12.7777777777777777No Hit
CCTCCACTCCATCCTGAAGGCTACCAACTACAATGGCCACACGTGTCTAC12.7777777777777777No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)