FastQCFastQC Report
Tue 10 May 2022
EGAF00003631512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086236
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTACTACTGTGCGGCAGATCATGGCAGTGCGACAACCTTCGTGGGTGT50870.46831443627351704No Hit
CTATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTTCGA50220.4623304696217028No Hit
GTAGTAATCACTCTCATCGTCTTGTTGGATGTTCTTAATGATCAGGGATC34160.31448046280918696No Hit
ATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTTCGATC29730.2736974285514382No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC29120.2680817060012741No Hit
GGCCTGAGTCGATCCCTGATCATTAAGAACATCCAACAAGACGATGAGAG28760.2647675090864232No Hit
GCTCTATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTT26970.24828858553758115No Hit
GGTATCAGCAGAGACCAGGGAAGGGCCCCCGATTTGTGATGCGAGTGGGC23560.21689577587190997No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21770.2004168523230679No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21670.19949624206894268No Hit
GTAATATAGAGCCGTGTCCTCAACTCTCAGGCTGTCCATTTGCAGATACA20980.1931440313154784No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20050.18458235595211353No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19740.18172846416432523No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT19420.17878251135112444No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA19000.17491594828379836No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18490.17022083598775956No Hit
GTCTTGGGCTCAGGCCTGAGTCGATCCCTGATCATTAAGAACATCCAACA18460.16994465291152197No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA18310.16856373753033413No Hit
GACCAGGGAAGGGCCCCCGATTTGTGATGCGAGTGGGCCCTAGTGGGATT18130.16690663907290865No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT17500.16110679447191956No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16600.15282130218479226No Hit
GTACATGGGGGAGAGACTGAAGAACCCAGCATTGCAGCAGCTCCACCATG16500.15190069193066702No Hit
GCCATACACTGGGTCCGCCAGACTCCAAGCAGGGGGCTGGAATGGGTGGC16270.1497832883461789No Hit
GCCTTGGGCTGACTCAGGACGGTCAGCCTGGTCCCTCCGCCAAAAATCAC16180.14895473911746618No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16020.14748176271086577No Hit
GTACATGGGGAGAGACTGAAGAACCCAGCATTGCAGCAGCTCCACCATGG15940.14674527450756558No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15510.142786650414827No Hit
CACCTGCACCCTGAGCAGCGGCTACACTAATTCTAAAGTGGACTGGTATC15440.14214222323693929No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15270.14057718580492637No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG14850.13671062273760032No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14290.13155520531449888No Hit
CCTCACAGGGTCCCTCTCCCAGCTTTTACTGACTCAGCCACCTTCTGCAT14180.1305425340349611No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13980.1287013135267106No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13860.1275965812217603No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG13840.12741245917093522No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG13780.12686009301846007No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT13760.12667597096763503No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC13430.12363795712902169No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13350.12290146892572147No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13030.11995551611252067No Hit
ACTCAGGCCTGAGCCCAAGACTGAGAAGCGATCAGGGATGCCATCCCCCC12720.11710162432473237No Hit
GATCATGGCAGTGCGACAACCTTCGTGGGTGTGATTTTTGGCGGAGGGAC12580.11581276996895704No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT12540.11544452586730691No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG12460.11470803766400672No Hit
GCCATGATCTGCCGCACAGTAGTAATCACTCTCATCGTCTTGTTGGATGT12090.11130177972374328No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA11780.10844788793595499No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT11750.1081717048597174No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11620.10697491152935458No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11530.10614636230064185No Hit
ATATAGAGCCGTGTCCTCAACTCTCAGGCTGTCCATTTGCAGATACAGCG11400.10494956897027902No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11310.10412101974156629No Hit
CCTGCACCCTGAGCAGCGGCTACACTAATTCTAAAGTGGACTGGTATCAG11250.10356865358909113No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTT11230.10338453153826607No Hit
CAGTAATATAGAGCCGTGTCCTCAACTCTCAGGCTGTCCATTTGCAGATA11220.10329247051285356No Hit
CCTCAGTCTCCTCACAGGGTCCCTCTCCCAGCTTTTACTGACTCAGCCAC11200.1031083484620285No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11060.10181949410625316No Hit
ATGCCATACACTGGGTCCGCCAGACTCCAAGCAGGGGGCTGGAATGGGTG11060.10181949410625316No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTG11010.10135918897919052No Hit
GCCTGAGAGTTGAGGACACGGCTCTATATTACTGTGTGAAGAGAAAGGGG11010.10135918897919052No Hit
TTACTACTGTGCGGCAGATCATGGCAGTGCGACAACCTTCGTGGGTGTGA10980.10108300590295294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)