FastQCFastQC Report
Tue 10 May 2022
EGAF00003631561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726791
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT16140.22207209500392822No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG15310.2106520306387944No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC14990.206249114257056No Hit
GACAGGATCCTCCCTGTCCCATGGCCTAAATGCCTCTCAGAACTCACAGA12900.1774925666388274No Hit
ACTTCAGACATCAAATCCTGCAGACAAGGGGAGCCCTCAGTCTGCAGGGC12840.17666701981725147No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC12250.16854914273842136No Hit
CAGTATAGATTCTCTGGCACTCTGAGGTCTCTGTGGCAGGCCTGGTCAGG11850.16304549726124842No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT10850.14928638356831606No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG10550.14515864946043636No Hit
GGACGCTCTCTGGCCTTCGGCCTCCATCCTGAGGCTCCACTTGGTCTGTG10460.14392032922807244No Hit
GCTCCACACAGTCAGGGCTTCCCTGAAATCTGCCTCTCTCTGTCATTATG10280.14144368876334462No Hit
ATCTCCACTGTGTCCTTAGCTCCACACAGTCAGGGCTTCCCTGAAATCTG10050.1382790926139702No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA9390.12919807757663482No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA8490.1168148752529957No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT8350.11488859933598518No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG8260.11365027910362126No Hit
GCTCCACACAGTCAGGGCTTCCCCGAAGTCTGCCTCTCTCTGTCATTATG8110.11158641204968142No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT8070.11103604750196411No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG7930.10910977158495358No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT7830.10773386021566034No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG7810.10745867794180171No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA7740.10649553998329643No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT7560.1040188995185686No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG7540.10374371724470996No Hit
GTGCAGAGCTAAGGACACAGTGGAGATTCTCTGGCACTCTGAGGTCTCTG7540.10374371724470996No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG7510.103330943833922No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC7380.10154225905384079No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG7350.10112948564305281No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG7330.10085430336919418No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG7300.1004415299584062No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT7290.10030393882147688No Hit
CCCTAACTCTTCCCCATAATCACTGACCAGCCTTGACACTCCCCTTGCAA7280.10016634768454755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)