FastQCFastQC Report
Tue 10 May 2022
EGAF00003631678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201798
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA160077.932189615357932Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT80904.008959454504009Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG66513.295870127553296Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG60202.9831812010029832Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG46392.2988334869522986Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40952.0292569797520295No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC33101.6402541155016401Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT32191.5951595159515952Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT31001.5361896550015361Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC24291.2036789264512036Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC21091.0451045104510452Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA18190.9013964459509014Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG16430.8141805171508141Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT15610.7735458230507735Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG14430.7150715071507151Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC13840.6858343492006859Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG13490.6684902724506685Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG13180.6531283759006531Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG12550.6219090377506219Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11740.5817698887005818No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11610.5753278030505753Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10560.5232955728005233Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC9610.4762187930504762Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG9290.4603613514504603Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC8700.43112419350043113Clontech Universal Primer Mix Long (96% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA8460.41923111230041926Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8150.40386921575040385No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC8130.4028781256504028Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC8060.3994093103003994Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7360.3647211568003647Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC7170.3553058008503553Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT6130.3037691156503038Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC5960.29534484980029535Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA5680.28146958840028147Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5420.26858541710026856Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT5320.2636299666002636Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT4980.2467814349002468Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4840.23984380420023987No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA4470.2215086373502215Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4430.2195264571502195Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4370.21655318685021657No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT4080.2021823804002022Clontech SMART CDS Primer II A (100% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT4060.2011912903002012Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG4050.2006957452502007Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4020.1992091101001992Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3870.19177593435019177No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA3870.19177593435019177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.1892982091001893No Hit
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG3760.18632493880018633Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3710.18384721355018385Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA3580.1774051279001774Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA3510.17393631255017392Clontech SMART CDS Primer II A (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA3370.166998681850167Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG3330.16501650165016502Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA3310.16402541155016404Clontech SMART CDS Primer II A (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC3250.16105214125016104Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT3200.1585744160001586Clontech SMART CDS Primer II A (100% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA3010.14915906005014914Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2910.14420360955014422Clontech Universal Primer Mix Long (95% over 22bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA2880.14271697440014272Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2820.13974370410013975Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2800.13875261400013875Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG2660.1318149833001318Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA2570.12735507785012734No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2550.12636398775012636Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG2510.12438180755012437Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.11645308675011644Clontech Universal Primer Mix Long (95% over 21bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2300.11397536150011398Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT2290.11347981645011347Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT2280.11298427140011297Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2270.11248872635011249Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2260.111993181300112No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATC2260.111993181300112Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT2230.1105065461501105Clontech SMART CDS Primer II A (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG2170.10753327585010754Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA2150.10654218575010654Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.10604664070010604No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)