FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT96.428571428571428Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53.571428571428571No Hit
TATTGTTGAGATTTTAAGGACTTGTTTGTTCACTAAAATGTAAAACAATT42.857142857142857No Hit
ATGTTGCTGGTAGCAGATGGTGTGGTGGCGGAGTTTCTGCTACCTTATTG32.142857142857143No Hit
TAGATAACGCGGCTTTGCGAATATTGCTTAACGCGATACCTTCAATATGG21.4285714285714286No Hit
TTCTGTGGCTGTGGGCTGCTCTGGGGGAGGGGAGCACGAAGTGGAGTCAG21.4285714285714286No Hit
CTCCTTGGTACATGTCATCCCCCAGAGGAGTATCCAAAGCTATTCCACTA21.4285714285714286No Hit
GTCTGAATGGTTCTTGAAAATTAGCCAGGATCAAATGCTATTGCAGACAA21.4285714285714286No Hit
GCTGAAGGCTATAAGATTTTGACCCTCTCTAAACTGCTCTCAAGATGAGT21.4285714285714286No Hit
CATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG21.4285714285714286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.4285714285714286No Hit
CCTATACATTGTCACCACCCATCATAGTAATTACGGGAACAGGCTGCCCA21.4285714285714286No Hit
GTGACAGGTCCAGCCAAGAAGACAGTGAAACTAACTCACGCATCATTCAG10.7142857142857143No Hit
GGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCG10.7142857142857143No Hit
GCACAGCTGCGCGCTGGCCGCCGTCGAATTGAGTGGCCAGCTCAGCGCCC10.7142857142857143No Hit
TATCAGAGCACTTGATCGCCTACAAGAAGTTCCCCATGAGGGTCCAATGT10.7142857142857143No Hit
GGCACATATTTGGGGCTGGAATCAGGTAGTTTCCATACTCCTTGAGCTTA10.7142857142857143No Hit
CACGATTTCGCTGGCGAGCGGGGCGTCGAAGCCGGCTTTCTCTGCCTGTG10.7142857142857143No Hit
TATCAGAGCACTTGATAGCCTACAAGAAGTTCCCCATGAGGGTCCAATGT10.7142857142857143No Hit
CCTTATGTCACATTCCCTGTTGCCTTCGTGGTCGGGGGTGTGGGTTACCA10.7142857142857143No Hit
GGCCGCGCCCGGGCAATTCGCACCGGGCACGAAAACCGGCAGAAACCGCC10.7142857142857143No Hit
CCACATAGCTGGAGCCCCTGTGCACTCCGGTGGTCACCTGTACTCCCAGC10.7142857142857143No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.7142857142857143Clontech Universal Primer Mix Long (96% over 26bp)
CTCTCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCCGGCCTCTCT10.7142857142857143No Hit
CACTTTTAACCATCAGATCTCGTGAGAACTCACTCACTGTCATGAGAACA10.7142857142857143No Hit
GCCTGATCCCATCCTCTGAACACTTAAATCCCCTGGCCTGAATGGTGGCT10.7142857142857143No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.7142857142857143Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAGAATGTTCTTGAAGACTTTTTTTCTGTTGGCTGCTGTCTGTGAG10.7142857142857143No Hit
CTCCCAAGGCTAAACCAGGAAGAAGTTGAATCTGAATAGACCAATAACAG10.7142857142857143No Hit
CCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAG10.7142857142857143No Hit
CCTCTGGCCTTGTACCAGGGAAACCGTGCACTGGGGAAAGGGAAATGATC10.7142857142857143No Hit
CCCCCTAAGCTTGTTTTTAGTGGGATGTGCATGGAGAGGATGACATTTGA10.7142857142857143No Hit
CCAGAATGGAGATGATCCCTTGCTGACTTACCGGTTCCCACCAAAGTTCA10.7142857142857143No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTAAATGGGAAGCAGTGGTATC10.7142857142857143Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTAGATGGGAAGGAGTGGTT10.7142857142857143Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGGGGGGGGGGGGGGGGGGGCTGAGGCGGGGGGGTGGGAGGGGGTGCGGG10.7142857142857143No Hit
GTTTTGCACCACTCAGTCTCCTCCTTTGAGTCTGTGTTTTATTCTTCATG10.7142857142857143No Hit
AGTATGTGCAGGTGGGCTTGCTCTTTCTTGCTCTCCGTACCGCTGTGCCG10.7142857142857143No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.7142857142857143Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG10.7142857142857143No Hit
ATGTAGCAGCTTTTGCTTATCTTGCTCTATCGGGAGACTTATCAGGAGAA10.7142857142857143No Hit
AGGCCCAGTGGTGTTAAATATATTTTCATTATTATGAATCTTCAGAAATT10.7142857142857143No Hit
ATCTTTTGATCGTGCGCGAGCTGACCGGCGGCAGCTATTTTGGCGAACGT10.7142857142857143No Hit
CGGTTGCTGGTAGCAGAGGGTGTGGTGGCGGAGTTTCTGCTACCTTATTG10.7142857142857143No Hit
AACCCTGTCCGTACTAAAAATACAAAAATTAGCTGGGCATGGTGGCAAGT10.7142857142857143No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.7142857142857143Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAGTAGCTCTGGCTTGGTGCTTGACCTGGT10.7142857142857143No Hit
ATCCTTTACAGCTCAGATTCTCAGATCGGCTTTAGTCACTTGGTCTCCTC10.7142857142857143No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT10.7142857142857143Clontech SMART CDS Primer II A (96% over 26bp)
CTGTATATATTATACAATGAAATATTATTCAGTCACAAAAAGAATGGACT10.7142857142857143No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGCGGGGGG10.7142857142857143No Hit
ATATTATATATATATAAATTTTCTTCCTTTCCTTTGTGGTTTTTCGCCCC10.7142857142857143No Hit
GCTTGGCCCCGGTGTGCTTTCGGAAGTGCCTGGTTAACTCATCACTTCTT10.7142857142857143No Hit
TTGCTTCTCCTTCAGTTTTGGAGAGTCAAAATTCAGAAGTGATTCGTAGT10.7142857142857143No Hit
CCGCCTAAGCTTGTTTTTCGTGGGCTGTGCATGGCGAGGATGACATTTGA10.7142857142857143No Hit
CGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGT10.7142857142857143No Hit
ATATTCATCAAGCTGGGCAAGCAGGGATCGACCCTGGCAGGAGTCATGGA10.7142857142857143No Hit
GTTACTACGGATAACTGCCGGACGATGTACTTGGTTCCGGAAGCTTGTTC10.7142857142857143No Hit
AAATTAATGTGGTGAAGAAAAAAGTGCAGGGGCTTGAAGTCAGAAGAGCT10.7142857142857143No Hit
GGGTACAATAAGGACTTGGGATAAGTCATTTCATTAATAATCAATACAAA10.7142857142857143No Hit
CCCCATTTGCTGACGTAGTGCTCTCATGGGCTCACCTGGGCCCAAGGCAC10.7142857142857143No Hit
GTCCCCGACCGAGAAATTCGCACCGGGCACGAAAACCGGCAGAAACAGCC10.7142857142857143No Hit
GTGCCCAGCTGGGCAATAAAGATTAACAGGCGATTTTAATCCTTTTTTGG10.7142857142857143No Hit
GTCTTAGTGTAGAAGACCAGTCTAGGACTGTCAGAAGAGAGTAGTCCTCC10.7142857142857143No Hit
GGGGGGCGCGGCCCCGCTTCGGTGTTGTAATGAGTTTAAGGGATAGGGCA10.7142857142857143No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA10.7142857142857143No Hit
GCTCATCACAGGTCGTGGTTTTCAGCCCCTGTCTTCAGGACGGCTTAACT10.7142857142857143No Hit
CGGATGCTATTTAAGAACCAGTTTCTTTTTGCAAGGTTAGAGAAGGGTGT10.7142857142857143No Hit
GGCCAGGAGTTCAAGACCAACATGGCCAACACGATGAAACCCCATCTCTC10.7142857142857143No Hit
GGGCCGGGGACGCAGTGGTATCAACGCGATTAAGAGGGACATATATGGAA10.7142857142857143No Hit
CCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCCG10.7142857142857143No Hit
GACGTGGGAGGCAGAGGTTGCAGTGAGCTGAGATCACACCACTGAACTCC10.7142857142857143No Hit
ATGCTGCTATGAACCTGGGTGTATGAATATCTCTTTGAGACCCTGCTTTA10.7142857142857143No Hit
CCCTCATCTACACCAACTATGAGGCGGGCACGGATGACTATGTGAAGGCA10.7142857142857143No Hit
ACATTGAGGACGACGACGAGGCTGCAATCCCCGAACGGCAACACCATGCC10.7142857142857143No Hit
ATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCT10.7142857142857143No Hit
AGTACATATTTAGTAATTCATGACTCAGTGAGGAACCATGCTTAACAACA10.7142857142857143No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.7142857142857143Clontech Universal Primer Mix Long (100% over 23bp)
CAACCGGCGTCTGCAGGCAGAGAACCGTGAGCTGCGCGACCTCTGCTGCT10.7142857142857143No Hit
CACTTGAGGACAGGAATTTGAGAAAAGCCTAGCCAACATGGTGAAACCCA10.7142857142857143No Hit
CTCCTGGATGTTTGCAAGGCCTCTGAACTGGTTTTTAAACATGAGTTTCT10.7142857142857143No Hit
ATTCAATTCCGTTCAAGGATTCCATTTGATTCCATTTTACGTTGATTCCA10.7142857142857143No Hit
GGGGGGGGGCGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.7142857142857143No Hit
CTCTTGGGCCCTGTGCTCTGGAAGAGGTAGCCCGTCTGGATGGACACAGC10.7142857142857143No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTTGATA10.7142857142857143Clontech SMART CDS Primer II A (96% over 25bp)
CCCATGTACTCTGCGTTGATCCCACTGCTTCCCATGTACTCTGCGTTGAT10.7142857142857143Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTTAACAGCTGCCTGCTGTTTTCTGCTCCCAGATGAAATGAAGCAACTCT10.7142857142857143No Hit
GGGGGGGGGTGGGCCGGGCGGCGGGGGGGCTGGCGGGGTGGTCGGTGCGA10.7142857142857143No Hit
CTGCTACCTTCATCACCAAATTGCACTCGCTCCTTCCTGGAATCCTTTTT10.7142857142857143No Hit
ACTTTGTGCAGAAGTGCGCAGCTCTCTCCTCAGGAGCCTTCTGGTTCCGC10.7142857142857143No Hit
TGCCCAGAGCCTGCTCATGGTAGGAGCATGTCAGTTCATCCGCCTAAAGA10.7142857142857143No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.7142857142857143Clontech SMART CDS Primer II A (96% over 26bp)
GTACCACCACCTTGTAGGAGCCCTGGAAGGGCTCTGATGAGAGGGGAAAA10.7142857142857143No Hit
GCCTCAGCCTCCCGAGTAGCCGGGATTAAAGGTGCCTGCCACAACGCCTG10.7142857142857143No Hit
CATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCG10.7142857142857143No Hit
GGTCGGTACACTTTCCTCTCCTTTGCTTACGTTTAGTTTGCTCTTCTTTT10.7142857142857143No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA10.7142857142857143No Hit
CAACAACATCGCCGTGCGCAAGAGCCGCGACAAGGCCAAGCGGCGCAACC10.7142857142857143No Hit
GCAAAAGATTTCCCAGCAAGGGTCCATTGAACAGAGTTGTATTTAATTCG10.7142857142857143No Hit
GGCTGAAAAAGATTTTATATGAAGAAAGAGGTTCTGCTTTTTCTTTCTTA10.7142857142857143No Hit
TCCTCATCGAAGTCTTCCCCAGTTATAAAGAGGTCACATAGTCGTGTGGG10.7142857142857143No Hit
ATGCACCACCACCCACGGCATCGAGAAATAGCTATCAATCTGTCAATCCT10.7142857142857143No Hit
CTGTTATTGAAGTCAGAAACTATGTCATCATCCTTAATGCCTCCCTCTTT10.7142857142857143No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG10.7142857142857143No Hit
GCCTCACCCCTGTAATCCCAGCACTTTGGGAGGCTGACATGGGTGGATTG10.7142857142857143No Hit
TCCCCATATAAAGTCAAACAGATCTCATCGTTTCCTTTATATTTACAGGC10.7142857142857143No Hit
CCCATGTACTCTGCGTTTATACCACTGCTTCCCATGTACTCTGCGTTGAT10.7142857142857143Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCCTGATTTTATACCGACAGCCGGAAGGGATCACATTATGGTCAGTGCGA10.7142857142857143No Hit
ACTGTGAACAGCTTGCTGTCACTTCTTCACCTCTTCCACTCCTTCTCTCA10.7142857142857143No Hit
ATTCAATTCCGTTCAATGATTCCATTTGATTCCATTTTACGTTGATTCCA10.7142857142857143No Hit
CCAGCACCTGGTCTCAGCTCAGCCTTATGCCTTTCCACTGACACCCCCCA10.7142857142857143No Hit
TCCTGATCCCATCCTCTGAACACTTAACTCCCCTGGCCTGAATGGTGGCT10.7142857142857143No Hit
TTCTAATTCCTTATCTAGTTATTCTGTGAATTATTGTCATCTGTGTCCTT10.7142857142857143No Hit
CACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.7142857142857143Clontech Universal Primer Mix Long (96% over 26bp)
GGAGGGCACTGAGCGAGGGCTGAATTCCAAGGTCAAGTCAATCCCAGTTG10.7142857142857143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)