FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGGATTACAGGCATGAGCCACCAAGCCTGGCCTAAGTGACTCTTA48.695652173913043No Hit
AAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGA24.3478260869565215No Hit
GAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTT24.3478260869565215No Hit
CGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAG24.3478260869565215No Hit
CAGCACACATTTGCAGAGACAGACACTCAGAGACACAAACTTGCAGAGAC24.3478260869565215No Hit
GCAGGAGACTGAGTAGTGTTGGTTCTTAGAAATCTATACACAATTAAAAT12.1739130434782608No Hit
CCCCAGGTCCTGAGGGTAGACTCAGGTCCTGAGGGAAAGGGGTGGACTTC12.1739130434782608No Hit
GCCCGTGCCGGTGGATTTGTTGCCCGGCAACCACGATCCGCTCGTGGCGG12.1739130434782608No Hit
GGCCTATAAGTAAAAACAGGTAATCATCAGACAAGCACTATAAAGAAGAT12.1739130434782608No Hit
AGTAATTAATAAGACAAGCAGACAAAGAATTCAATAGGGACGTGGAAAAC12.1739130434782608No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG12.1739130434782608No Hit
CTCGAGGACGGCACGCTTGTCACCGTCAACGGACGCGGCATCGGCGAGCG12.1739130434782608No Hit
TGCCTGTAATCTCAGCTACTCGGAAGACTGAGGCAGAATTGCTTGAACCC12.1739130434782608No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC12.1739130434782608No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAATCAAAGTCTTTG12.1739130434782608No Hit
TCATAGCTAACTGCAGCCTCGACCTCCTGGGATCAAGCGATCCTCACACC12.1739130434782608No Hit
TCGTGGTCCCGCTCAAGGCGGCGGGCGTGGACGTGGAGATCGTGCCCGGC12.1739130434782608No Hit
ACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAAT12.1739130434782608No Hit
ACCCATAGGACGCATTTACCAAGTTGCAGCCAGTGACCACCGTGCCGTCG12.1739130434782608No Hit
ATTCTAAGAGGCCCAAATTTAAGAATCTTAACAAATCACATTTCAGATAG12.1739130434782608No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCTAAAGACCC12.1739130434782608No Hit
CTTCATCCGGCGACTAGCACCGCGTCCGGCAGCGCCAGCCCTACACTCGC12.1739130434782608No Hit
TTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCT12.1739130434782608No Hit
GCCGGGCATTCTCCGCCTCGAGCTCCTTCATGCGCGACATCATCGACGCG12.1739130434782608No Hit
GGATGGAACAGCACAACGGTAAGTCCTTCGCGCCGTCCGCGGAGGCGCTG12.1739130434782608No Hit
ATGGTTTTCTCTCTGCTCCTCTCATGCTTCTCCTGGGGCTCTGATGACTT12.1739130434782608No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC12.1739130434782608Clontech SMART CDS Primer II A (96% over 26bp)
GTATTCATGATGGTGGGGGATGGAGTGTCTTCCGAGGTTCTTGGGGGAAA12.1739130434782608No Hit
ACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC12.1739130434782608Clontech SMART CDS Primer II A (96% over 26bp)
GCCCAGGAGGCAGAGGTTGCAGTGAGCTGAGAAAACACCATTGCACTCCA12.1739130434782608No Hit
CTTCCATCATTATTCAGTGATGATGGAGGAGACTTTAGATAGAATTTTTT12.1739130434782608No Hit
GGGAAATTCCATGGGAAAGTGAAACAAGCATGAGTCAAGTCAACCAGGGA12.1739130434782608No Hit
CCGCTCAGGGCAGGCAGCAAGTTAACAGCGCCTGTGAATTGGCTCTTCAA12.1739130434782608No Hit
CGGTATCAACGCAGAGTACATGGGGATTGCTGGGGAAGAGGAACTGTGCC12.1739130434782608No Hit
GAATAAAAGCCAACACCAAACTGCCCAATCATGGAGATGTCTGCACCAGC12.1739130434782608No Hit
ATCTGCATCAATTGAGATGATCATAAAATTTTTTTTCCCTTCATTATGTG12.1739130434782608No Hit
CTTCCGCTCCGACCAGGGTGTCAAGCCCTCACAGAAGGTGCCGGCCCGCG12.1739130434782608No Hit
GAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCG12.1739130434782608No Hit
CCCTAGGCCTCAGCGTGATCAACCCGGTCACCGGCCCCGCAGACGCCCAG12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)