FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCGGTCAGGTCGTTGTTGATGACGCCATCCATGTTGCGACTGAGCGG34.285714285714286No Hit
CCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGG22.857142857142857No Hit
GCATTGATCTGGCGGTGATGCTTGCCAAGCAGACCCGCCTGTCCCAGCAC22.857142857142857No Hit
CAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAA22.857142857142857No Hit
GCTCAAGACAAGCCTGGACAACATGGCAAAATCCCGTCTCTACTAAAAAT22.857142857142857No Hit
GTACATGGGTAGGTCACTTGGTTTCGGGTCTGCGTCAGCCAACTGCTCGC22.857142857142857No Hit
GGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGC22.857142857142857No Hit
GGTCCATAACTGCTGGGTCAAGCTCACTCATCAGAATTTCCAAGGTTTGA22.857142857142857No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT22.857142857142857No Hit
ATTGATCTTCTCCAGATGCACAGTCACCACCTTGCCGTCCTCAATGAGCC22.857142857142857No Hit
GAATTCATGTTCTTCCTGGCTGTTTGAGTCAGCACATCAATCTCCTCCTG11.4285714285714286No Hit
CTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATT11.4285714285714286No Hit
CCTGAACACTGACCTGGGCAAAGAGTTTATGACCAAGTCTTCCAAAGGAA11.4285714285714286No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.4285714285714286Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGG11.4285714285714286No Hit
CAGTTACACCACTGTCGGCCAAAGATGCACTCCTCCTTTAATCAATTTAA11.4285714285714286No Hit
GCTTAGCATGAAAGAAAGCCCAGGTAAAATTTGTAAACTAGACAGTTGAT11.4285714285714286No Hit
GCTTAGATGCAGGTAACTTCAATTGCTCACCCCTTATATTTTCCTTATTT11.4285714285714286No Hit
GCCTTCTGCAGCTCGCGCACCTCATCCACCGGGCCCACCATGAGGGAAGT11.4285714285714286No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCTTTATGAACATCGCGTA11.4285714285714286No Hit
TGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTG11.4285714285714286No Hit
GATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACT11.4285714285714286No Hit
CGCCTGGGAGGCGCTGCGGGCGGCGACCGAGGAGATCGAGCGCCTGCGCG11.4285714285714286No Hit
AGGCAAGGAAGTGTGGCGGATGATCGGGGGGATGGACTGGAACGGTCCGC11.4285714285714286No Hit
GGTCAGAACCGAACTGGGCAAAAGCAGCAACCTCACGATACTGAGCCAAT11.4285714285714286No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACTGTCTCTTA11.4285714285714286Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCG11.4285714285714286No Hit
GCACGTAGGCACCAGCGAGCGCGACGAACAGCGGCGTGGTGTCGACACCG11.4285714285714286No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.4285714285714286No Hit
TCTTCCTGGCATCCTTGGGGGTAGGGGAAAGACAGACAGCTCCCAGGGCC11.4285714285714286No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGATTATCAAACACCTGAGC11.4285714285714286No Hit
GTTGTATGTGGGATGTGACTGGAGAAGATGGACATCACTGGAGGGTGTTC11.4285714285714286No Hit
TCTCCCTTCTGTGTGTTCAAGTGTTCTCGACTTGGATTCTTAACTATTTT11.4285714285714286No Hit
TCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGT11.4285714285714286No Hit
TTATTGTACAATTACCCACCACTGGATTTGACTCAGAGAGGACCCCCAGA11.4285714285714286No Hit
CCCTATTGCAACCAAACATTGAAAGAGTTGCTGACGAATTTTATAATTTG11.4285714285714286No Hit
ATCCCAGAGTCCTTTCACCCGGAACCAAATGCCGGGCGCGGTCGGCTGGA11.4285714285714286No Hit
TCGATATAGAAATATCATTCAATATGATTTCCAGAAGGACAAGTTTCCTG11.4285714285714286No Hit
GCAATGGCGCAATCTCAGCTCACCGCAACTTCCACCTTCTGGGTTCAAGC11.4285714285714286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.4285714285714286No Hit
CTCTGGCATGATGGGATGGCGACCGGGCAAGCTTTCTTCCTCGAGATGCT11.4285714285714286No Hit
GGGGGCCCCCTGGTCTGTAACAGAACACTGTATGGCATCGTCTCCTGGGG11.4285714285714286No Hit
TTCCAGAACTATATTCCAAACATATAGATTAACTGACCATCAGTGAGAAA11.4285714285714286No Hit
CTGAAAGTGCTGGGATTACAGGTATGAGCCACCGCGCCCGGCCCCGTTGT11.4285714285714286No Hit
ATATTACGTAAGTAAAATAAAAGTTTCCTCTATAATTTGATCATGAAATG11.4285714285714286No Hit
GAGCGAGAACTATGCCTTTGCGGGCATGTATCATGTTTTTGACCAGCACG11.4285714285714286No Hit
ATCCAGCTCCTAGCATCAAGATCAAAATCAAGACAAGAATTCCCTCAAAA11.4285714285714286No Hit
CGTCGTCGAGTCGGCCGACGGCCTGACGCTGGTCGACACCGGCTTCGGCC11.4285714285714286No Hit
CTGCTGGACAAGCTCGTCCTCCCTGTGCTGCCCGGGGCCCTCCAGGCGAG11.4285714285714286No Hit
GATCCAGCGGGCGATAACGCACCTCAACGGGGTACGTGCGCCCCGAGACC11.4285714285714286No Hit
GGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGG11.4285714285714286No Hit
AGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAG11.4285714285714286No Hit
GGATATGAGAATTAAGCTAAATCCAGAAAGAAATAGGAAAAAAAAGGATA11.4285714285714286No Hit
GGATGAGCAAGCAGGAGGCGTTCCTGTTGAATGAAGACTTGGGAGATTCC11.4285714285714286No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG11.4285714285714286No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATCTGCGTTGATACC11.4285714285714286No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC11.4285714285714286No Hit
GGCATCTTGTATGTGTTTTCTTAAAAAATCTTCAATCTCATCCTGGAACA11.4285714285714286No Hit
GCTTAGATGCAGGTAACTTCAATTGCTCACCCATTATATTTTCCTTATTT11.4285714285714286No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)