FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTGGACTGTCCTCATCCTCTTTAGCTGCTGTTGCGCCTGTCTCTTAT24.651162790697675No Hit
CCTCGAGCAGGCCGGAGACCTTCTCGAGCTGTCTCTTATACACATCTCCG12.3255813953488373No Hit
GACGGAGACGGCGAGCAGGGCGGCGGCGAACCCTATTTCGACGGCCCGCG12.3255813953488373No Hit
CCCTTACACTTCAGCACCAGTGGCTCGCCAATCCGGGCTGTGATGTTTTG12.3255813953488373No Hit
ATCGATGAGGTCACGAACTTCGTCGGTGATCAGAGCCGGACCGTCGAAGT12.3255813953488373No Hit
AATCGGTGCGATCGTCTAAACTATGAGGTTCCCGTTGGCTTTGGCCAAGC12.3255813953488373No Hit
AACCTGCACCAGCTCCCAGCCGTCGTCACCCCACTGGTCGAGGATCTGTT12.3255813953488373No Hit
CAAACGCCCTCTGAAATCTGTAGCAACGACGTTCGTGGCGCTGGGCCTTG12.3255813953488373No Hit
TCTAATCGCAACCTTTCGTTTTTTAGCCAATGCAGCGAAGAAGTCATTCT12.3255813953488373No Hit
CAACAATGCCGCCGAAGATCGAAACTGAATCATCCTCATAAGCGTAATCC12.3255813953488373No Hit
GATAAAGAGTGTGTCTACTTCTGCCACCTGGACATCATTTGGGTCAACAC12.3255813953488373No Hit
CCTCTGGCAGGCCCCCAGTCAGATCAAGGGAAGCCACAGACATCTCTTCT12.3255813953488373No Hit
CCGGTTTCCCATGCGACAACTTGTCGGAACATGTAGAGCCCCGACCACCC12.3255813953488373No Hit
GTTCAGTGGTGTTAAGTACATTCACATTATTGTGCAACCACTGTCTCTTA12.3255813953488373No Hit
GGGCGGTCCCGACTTCACGCTGCCGCTCATCGGGCACGCCGCCTGGTGGG12.3255813953488373No Hit
GACGTTGACCTGGTCGGCGTCGCCGCCGCGGTACCACGTGGTGACGTCGA12.3255813953488373No Hit
TTTTAGAGCAGGTCTGGTTAGACTTGCATGCTAGTGCTGTCTCTTATACA12.3255813953488373No Hit
ATTCTCTAAGACTCATAGAAGAAAAGGAGTATAAAGAAATCATTTGCAAA12.3255813953488373No Hit
GGGCTGTGGAACGGCAGCGGGCAGATGCTGTCTCTTATACACATCTCCGA12.3255813953488373No Hit
CCTTGGTGGCCCTGGAAAGCGGCTGCAAGGTGGCCTTAGTCAGGGGAGGC12.3255813953488373No Hit
CACCTCGGCTGACTTGGCTTTTCCCTCCGGTGATAGCTCGGTAATCTACC12.3255813953488373No Hit
GGCCAAGAAGGATGTCCACATGCCTAAGCACCCGGAGCTGGCACTGTCTC12.3255813953488373No Hit
TAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCG12.3255813953488373No Hit
GTGACACCATTCACCATCACCACTAGTGATCCAGTAGCTGTCTCTTATAC12.3255813953488373No Hit
TTCCTACCTTTGCCATGAAAATTTGCTGCAGGCAGAAATCTGACATGGGA12.3255813953488373No Hit
GGCCAGAATCATGCAGATCCACTCCCGAAAGATGAATGTCCTGTCTCTTA12.3255813953488373No Hit
TCTGCTGGGAGGGCGGCGTCCTGACCGTCCGGGCCCTGTCTCTTATACAC12.3255813953488373No Hit
ATGTTCCCCCATCTTCTGGGCGTTCAAAAATTGTAAATCCTGCTTTGCTT12.3255813953488373No Hit
GGCCGAAAGCAACCAACACACGCTCATCGTGTAGCTTGAGCAGCAGATCC12.3255813953488373No Hit
GAAGTCTTCGGCGACCGCGTCTTCGACACCGTGATCACCCTGTCTCTTAT12.3255813953488373No Hit
GTGGTGGCAGATACGGATTGTGAGATTATTCTGCTAAATGTCCAAAGGCT12.3255813953488373No Hit
GCAGTGGAAAGGCGGCGCCCGTCGCTGGCTGCTCCACGACCGCTGTCTCT12.3255813953488373No Hit
GTGTTGTGGACACGGACGGGGAATGGGCTGGGATCTTGAACGGTGAGGGG12.3255813953488373No Hit
AGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAAC12.3255813953488373No Hit
GATCTTATGTTCCGAATGAGGGACAACCCGCTAGCCATTGCTGAAGGTGC12.3255813953488373No Hit
AACTTTCCGATACGCAGATCGATGCGCTCACCGCCTTCCTGCGCAGCCGC12.3255813953488373No Hit
CTATGAAGCTATGCGCCCCAGCGCCATCCCGCAGATGATGCTGGACCTGA12.3255813953488373No Hit
TTATTAATTCATTCAATAAATTTTTGTTGGCTCACTGCCCTAACTGCCAC12.3255813953488373No Hit
CCTCAAGCATCCGGCCGCGTCGCAGGTCCTTCTCCCGGACCCGGACGTCG12.3255813953488373No Hit
CGACAGCTACGGCTCCGTGCACCCCATGTACCTGTCTCTTATACACATCT12.3255813953488373No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT12.3255813953488373No Hit
GGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTG12.3255813953488373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)