FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCACACCACGCGTAAACCCCCTGACAAGGCACGAGGCCCGCCCCAGA22.857142857142857No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT22.857142857142857Clontech Universal Primer Mix Long (96% over 26bp)
GCGTAGTGCTGGCTGATGTTGCCGCCCTTGCTGTGGGCCATGATGGTGGC22.857142857142857No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22.857142857142857Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCCTGCGCTGCTACTGCATGACAGATGATAAAGTGGACAAGACCCTGGA22.857142857142857No Hit
CAGTGGATCGTGCCGGGCCGAGCCGTCACGCATAGCCAGATCGTCAACGA22.857142857142857No Hit
GTATTTGTCCGCCAAGCTCGGATTGGTTACCGGACATTCCTTGCCCGAAC22.857142857142857No Hit
CCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGA22.857142857142857No Hit
ACGCAGAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.4285714285714286Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGCATCGAGACGGTGCACCAGGCCGGTGGCATTACCTGGGTCGATGATT11.4285714285714286No Hit
TGCCAGGAGTGATTCTGGAAAAGTAAGCACAGAAAATGGAGACGCACCCA11.4285714285714286No Hit
TCTCCGCCCTCGGTCGGGGCAGGTACTGCCGCTGTCGGGTCCGCGCCAGC11.4285714285714286No Hit
TCGTTGCCGTTGACGACGAAGTCGTAAGCGTAAGCCAAAGCTGAACCCGG11.4285714285714286No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11.4285714285714286No Hit
GCAACTAGTGACTCAAACGCCGTAGGGTGCGGCTGGATGACCTCGGGATC11.4285714285714286No Hit
TGCACGCATCGGCGACTACACCGACTTCTACACCTCGATCGACCACGCGC11.4285714285714286No Hit
GAATTACAGGCTTAGGACTCTTGATAAATTTGTCACCTATTTCACAATAA11.4285714285714286No Hit
CTTCCTACTTCAGGTCGCTAGGAAGCTCGGGGGACTTACCACCCCAGGCG11.4285714285714286No Hit
GTGGCAAACGCGGCGGTCGAAACAGCCACCGTCGACAGCAGGCTTCCGAC11.4285714285714286No Hit
CCATTGCGGGAGCCATCCTCAGGCATCCCTCACCCCCGGTCGTGGGAGCG11.4285714285714286No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.4285714285714286Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.4285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
GACCTCGACGCCTTCGCCCTGGCCTTGCTCCTGGCGGGCAGTGACGCCGT11.4285714285714286No Hit
GATTTTTTATTGAAGGGCGAGTCTCCGGCATCTGATGGCAGCTACAACTG11.4285714285714286No Hit
AGTAAGGCCTGCGCGAGCAAAATCACCACGAGATGGCCGAGACCGCGCAG11.4285714285714286No Hit
TGTTGACGTGATCAACCCGTAACGTGACGGCCAGTCCCTTGGGCAGCCGG11.4285714285714286No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGCTTCCCATGTACTCTGCGTTG11.4285714285714286No Hit
CCCTCGTCGTCCCTAACGCTGCCAATGGCGGCGTCGTCCTGGCTGGGGTT11.4285714285714286No Hit
TTTCGATTCCATTAACCTCGGGGATTCTTCGGTTCCCGCAGATGCGCAGG11.4285714285714286No Hit
GTGCTGGGATTACAGGCATGAACCAATGTGCCCGGCCCACAATTTTATTC11.4285714285714286No Hit
AGTCCTTGGTGTAGGACCGCTGTTTTCCTCCGGCCGCTGCTGCTGCCTTC11.4285714285714286No Hit
GGTGATCAGATCATAGGGACAGATTTATTTTGAAGTGTTTAGACCATCCC11.4285714285714286No Hit
GTGTGGGCCGAATCAACCCATGCCGACGACCTGGATCGTCACTCCTTTAC11.4285714285714286No Hit
GTTTAGAGATAAGGTGATGTCAGAGTTTAATAACAACTTCCGGCAGCAGA11.4285714285714286No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11.4285714285714286No Hit
CAAGAAACCCCGATCACAAAGATACGAATCTCTTAAGGGGGTGGACCCCA11.4285714285714286No Hit
TCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGACGGAGACCTAA11.4285714285714286No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.4285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
TCGACGTCGGTTCCTCTCAGTTCGAGCGCGATCAGGAGATCTCCGTCACC11.4285714285714286No Hit
TTAACACACCACGCGTAAACCCCCTGACAAGGCACGAGGCCCGCCCCAGA11.4285714285714286No Hit
GAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCT11.4285714285714286No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.4285714285714286No Hit
GTATTTGTCCGCCAAGCTCGGATGGGTTACCGGACATTCCTTGCCCGAAC11.4285714285714286No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.4285714285714286Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTTATTTGCCACCCAGTTTGATGAATGAGACAGATTTGGGAGTTAGCT11.4285714285714286No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.4285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
TCGAACCATTACCCAACGACACCGACACAACAGCCGGGACGACTGACACC11.4285714285714286No Hit
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA11.4285714285714286Clontech SMART CDS Primer II A (96% over 26bp)
ACATCCGCTACGGCCGGGCGTCGGCGACGGATGCCGAGATCCTCGAGGCG11.4285714285714286No Hit
GAGCGGGTTGGCCTGGGCATCGTTCTGGGCGTTAGCGATCGCGTTCTCAT11.4285714285714286No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG11.4285714285714286No Hit
GGGCTGGGGCGGCTGGTGCCGACTTAGTTGGTCATGTGGTAGCTCTCATC11.4285714285714286No Hit
GTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTAT11.4285714285714286No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11.4285714285714286Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGCTTCCCATGTCCTCTGCGTTG11.4285714285714286No Hit
GCTTATTTGAGTCAAAAGTGGGCATTGACGCCATCGCCAGGACCTCACCA11.4285714285714286No Hit
ATGCTCTTCTTCTCACTGTCTTTCTTTTATTTTCTTTTTGTTGAGATGGA11.4285714285714286No Hit
ATTCACGATGCAGTGCGTCCGCTGGTTCCGGCATACGTCGTCGAGAATGT11.4285714285714286No Hit
GGTTGTAGGTAGTCGGTAGTCAGGGTACTGGGTACACGGATGTTCTTTGT11.4285714285714286No Hit
ATTTACGGCCGCGCCCCGGAGCGGATAAAAGAGTACGGCCGCAGGACTGC11.4285714285714286No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11.4285714285714286No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGG11.4285714285714286Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)