FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGCGACGGGATCGCCAAAATCGCCAGCGACAACGCCCCGGTCATAAG34.285714285714286No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34.285714285714286Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34.285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
GCCTAGGTATCGTGACGATCCTTGTAGCGGTGGTGCTCTTCGTGATCGCC34.285714285714286No Hit
TCTCTAAGATGCTTCTCAAGAGGGGTCTCAAAGACAGACAGCTGTGGAGC22.857142857142857No Hit
GCACCGAAGCTGTTCATGCTGCACGGCTCGCGTGACCTGTCCGCCTCCTG22.857142857142857No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG22.857142857142857No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC22.857142857142857Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACGAAACTGATGGGTTTAGCCCAGCGCGGCATTTGGAGGTTATCGCCGA22.857142857142857No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAACCTCAC11.4285714285714286No Hit
CGGCGAGTCCCAGCAGTGGATCTCGGCCAGCATGCGCCTGCTGTCACTGG11.4285714285714286No Hit
TTCACGACCGTTTCCCGCGCGGGCGCCGTCGTCACCTGGCATGGCCGTTG11.4285714285714286No Hit
GTAGAGGAACATGAGGACGGGGGTCGACCAGGCGATGATCGTCGTCAGAC11.4285714285714286No Hit
CGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG11.4285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.4285714285714286Clontech SMART CDS Primer II A (96% over 26bp)
GCCATACCGACATCGCCGGGAACACGCGGGTTGTCAGAGTCGTAGCCGCG11.4285714285714286No Hit
CGCCTGGCCACCACAGAGAAGAAGACGGAGCAGCAGCGGCGGCGGGAGAA11.4285714285714286No Hit
ACCGGCAGCCGCTTTCGCAAACACCTCTGCGATGTCGAGATCGACCGGCT11.4285714285714286No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11.4285714285714286Clontech SMART CDS Primer II A (96% over 26bp)
GCCCCCTTTGATGCTGATAACTTTGTCACCGGTCTCATGGGTGACGATTC11.4285714285714286No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.4285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
TAGCCGAGCACACCTATGGCGACCATGACGACGCGCCAGCGCTTCAGCTG11.4285714285714286No Hit
CCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTT11.4285714285714286No Hit
CTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGTAACTCCT11.4285714285714286No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.4285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
CCTTGGATCCTGCCATCCGTCTTTTCTCCCGGGACTGCCTTTGCTTTGTG11.4285714285714286No Hit
GACCTGAGTGCTCTCCATATTGTGGGGTTGAAGTCTGGATGGGAGCTTGC11.4285714285714286No Hit
GAGTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT11.4285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
GTGGATGGAAGAGAACATCGGCAAGCGCATCAACGTCGACCGCGTCGACG11.4285714285714286No Hit
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGCCCTTACAAGTCCGCTC11.4285714285714286No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACGCAG11.4285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
AAGTTGAGCGTCTCATGGGATTCGCGACGCTGATGCAGGCCGTCACTGGA11.4285714285714286No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA11.4285714285714286No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.4285714285714286Clontech Universal Primer Mix Long (96% over 26bp)
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC11.4285714285714286No Hit
GGCGGACACCTCGGAGGCGAGCCTTGCGCTCTCCGCACGAGCGGCCGCAG11.4285714285714286No Hit
TCCTCAGCCAGCCCCCAATCTTCTCCACACAGCCCTCCTTATGGATCGCC11.4285714285714286No Hit
TACCAGGCCTCTCGTGAGCAACAAATCGCCGTCGCCGAGCGCGTGCTTGC11.4285714285714286No Hit
GTGTCGTGCCTGATACTGCATGACCAGGTGTAAGGGACGATCAGCGATAG11.4285714285714286No Hit
AGCCTGGTGATGCCATCTTCTTTGCTGCTGGCTCGAAGACCTCCTCCCAA11.4285714285714286No Hit
CTTCTCGACGATGCCGCCGTCCTCCATCACGAGGATGAGGTCGGCGTCGC11.4285714285714286No Hit
CCTCTGGAAGCTTTGAGTAAAACGACATCGTGTGAGGTCCAACCTAGTGA11.4285714285714286No Hit
TCCCAAGGGTTCCAACGGGGAAACCTACATTAAATCCATTGAGCACAACG11.4285714285714286No Hit
GCTTAATTTGACTCAACACGGGAAACCTCACCCGGCCCGGACACGGACAG11.4285714285714286No Hit
AAGGGAAAGCTGAGCCGCTACTCGGTAGTACGCCACCACGTTGACAGCTA11.4285714285714286No Hit
AAGATGTGTTCCTCCGCGCGTTCGAGCGGAATCGCATCGCCCTGCGCGTT11.4285714285714286No Hit
CTACTTGCTGGAGCTATCAAGGTGTCCGGGCCACCACCACAGCTACCGGA11.4285714285714286No Hit
CTTCTGCAGAACGTGGGAGAACCTGGCAGCCACTTGATGTCCTTGTGCAT11.4285714285714286No Hit
ATCCTTGAGGGCAGTTTTCTGGCGACAATCGTGAAAATGAGTGACAAACT11.4285714285714286No Hit
GGGTCGCACCAGTCCTTCTGTCCTCATGTTGGCAGAGATATCTACTCTGA11.4285714285714286No Hit
GGTATTGTGTACCTGGGCCATATCCCGCCGCGCTTCCGGCCCCTGCACGT11.4285714285714286No Hit
CTTTAATGCATGACGGGGACTCCCAATTTCAAGGACTCTCCATAATTAAT11.4285714285714286No Hit
ATGATGCCGCGGTCAGTTCCCAGCACTACGACGCCACGCAGCTTGCCAGC11.4285714285714286No Hit
ATGTAAAGTACCAAATAGCTAATTCTGTTTGAGCTCTGTATTAGACTGTT11.4285714285714286No Hit
TACTTATACACTAAAGAAAATAAACATAAAATGGTCTCCCGCCTGACTCA11.4285714285714286No Hit
ATTCTCGAGGTATCGCTGATGCCGTCAGCCACCTGGTTGCCCAACTTGAT11.4285714285714286No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC11.4285714285714286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)