FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003631992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003631992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTGACGTTCTCCGGCGGCCGCGCGCTCGGCGACAACACCGACGGCGCG32.5423728813559325No Hit
ACGGTACACGTACGATGAGCGGCTTTGTGAGCGGCTCAAGGCTCGGTTGG32.5423728813559325No Hit
CGACAGTGCCATGCTGGAAAGGATCAACATTGCGTCCTCGTCGCGGCGGC21.694915254237288No Hit
CACCAGGCCCAGTGCAGGAGAGGGAACTGCAGGGCAAGCACCAACAGCCC21.694915254237288No Hit
TCTCAGCTCACTGCAGCCTCTGCCTCCTGGGTTCAAGTGATTCTCCTGCT21.694915254237288No Hit
CAGTTCTACTCGTCGCGGTTCGGCGTCTGGGCCTGTCTCTTATACACATC21.694915254237288No Hit
CCCCTACCCGTTCGCCCGACGCATGTCGACGCTCGATCACCTCACCAAGG21.694915254237288No Hit
GCGGAGAACATCGCGCACAACCCCACCGCCGCCCGTGCCGCCTACCGCCG21.694915254237288No Hit
GACATGTACAAGACAAGCAAAAAGTACCTGCGATGATACCGCGGGTCTTC21.694915254237288No Hit
CGATGGCCATCTCGACCAGCCGCTCGGTGGCGCCGATGGCCCGCGCGGGC21.694915254237288No Hit
CCACCTGCCGCCACTCGGCCTTTCCAATGCCTGGGGTTATAATCCCGTCG21.694915254237288No Hit
GTGAAATAAAATGTGAGTTAAGATTGTGCCACTGCATTGAAGTCTGGGGG21.694915254237288No Hit
GATCACACCTGCGTTGGGATCGACGAACAGCACCGGTCCGCAATCGGCCG21.694915254237288No Hit
CCATACGAGATGCTAATGCCGATGCTTCTGTAATGAAACTCAATCAATTC10.847457627118644No Hit
CATTAAGTGAGTCTGAATCATTGAGCGAAAGCGAATCCTTGAGTGAGAGC10.847457627118644No Hit
GGGCATGATGGCCCTCGCGGGATCGTCGCCTGTCTCTTATACACATCTGA10.847457627118644No Hit
TTGACATGCGGATCGATCGGCACGATGCCCGCGAACATGAGCTGCACCTG10.847457627118644No Hit
GATGTAGAGTCAATCAAATTGCTGAGACAGAGAGTAAGCTGTCTCTTATA10.847457627118644No Hit
GGTTCGGGGCGCGCTGACCGGTTCACGCTGACCGGTCCGGTTCAGTGGCG10.847457627118644No Hit
GTAGAAGTTTTGTGTCTCGATATCCCGAAACTCCTCGATCGACCAGCTTC10.847457627118644No Hit
GTCGTACAGAAGGACGCGTAGCCGCTTCGTACACTAGGGTCCATGCCCGG10.847457627118644No Hit
GTCCTACATCCTACGCCTTACCTGGGGATGTTTCTGTCTCTTATACACAT10.847457627118644No Hit
ATACTGGAATATGCCTGCCCCGAAGCCAGCAGCAAACAAGAAGGTGTTGT10.847457627118644No Hit
TGCCGCTGCCGGCCCACGGGCCCCAGGCGATGGAGGTCGCCGGCCTGTCT10.847457627118644No Hit
CAGTGATGGGGCGGCCAATTTCTGGGTCTTCGACGCGGGAGAGTGCCTCG10.847457627118644No Hit
CTGATGAGGGGCCCGGGTGAGGCCCCGAGCTAGAGCTGCTAGAGCTGTGG10.847457627118644No Hit
CCAGCTACGGCGAGCTGGCGGAGCGGATCGGGCGGCCGACCGCCTCGCGG10.847457627118644No Hit
GGTAATCTTCTATCAAGATCTTCTGATGCTTGCTCTCTAATAACTGCTCA10.847457627118644No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.847457627118644No Hit
ACCTCGGCACGAACACCATGCTGAAGTGCTTGTCGGCGAACAGCTCGTCC10.847457627118644No Hit
TGGCAGATGTGTTTGCACCACCCCGAGCTGCTACGCCACGTGGCACAAGC10.847457627118644No Hit
CGCCGTAGAGGCCTCCTACCAGACGGCCTTGCGCGTTCCACGTCTCAACT10.847457627118644No Hit
GCGTCATCTCGATCGACGACATGCCGGACCGCCCGTTCCTTTGATGCATC10.847457627118644No Hit
ATTAGGAGTTGGGAGTTCCTTTGAAAAAGAAAACAGAAAATTTAAGAAAA10.847457627118644No Hit
AGGAAGAGTTGCATGTCTCTCACTTTAAATCAAAAGCTAGAAATTATTAA10.847457627118644No Hit
AAAGAATAATCTGCGTTCGGGTCAGGGTCCGGCGCGTGTGTCGGCTGGGG10.847457627118644No Hit
GGTAGGAGAGGGTCGCGGCTGTGGGCCAACTGCTCGTCCCCGCGGTCAGG10.847457627118644No Hit
CGGTATAGGCAGGCTAAAAATCTTGCCATATGAATTACGCCTGTCTCTTA10.847457627118644No Hit
GGCCTGGGCCTGGCCAACCTGCTCACTGATGACAGGGCAAGGCGAGAGAG10.847457627118644No Hit
GCTGGACCACCCGCAGTGGGACCAGTTTGCCGGCCGCGATGCAGACCGCG10.847457627118644No Hit
AAAAAAGACATCTCTAGCAACAGATTTTTTACCATTGTTAAAAACGGATA10.847457627118644No Hit
GTTCGCCACCGAACTGATCCCCGATGCCGACGGTTCCGTCGCCGAGCGGC10.847457627118644No Hit
GCCTCGGCCTGTCCGACGACCCGGCGGCCCAGCTCGTCCTCCATCCGCAG10.847457627118644No Hit
GGCTGGGCGGAGCTCGAGGACACCGCGGGCACCGGCAGTGTGTTCAGCAT10.847457627118644No Hit
ATGCTGATGTACCGCTGGCCGGAAGAACACGGGCCGGAATATGAAGAAGC10.847457627118644No Hit
CTTGTACTTCGATGGGTGACGCTCGGAATTACTTTTGCCGTCGGCATCTC10.847457627118644No Hit
ACCTCGCGCAGAGCGTATCCAACTGGCGCGCAGAGCGCAGCCTGAGCGAG10.847457627118644No Hit
GCGGCAGGTAGAGCAGCGAGGAGGCGAAGATGACCGGGATGACGCCTGCC10.847457627118644No Hit
GCCCCGCCCTGCCGAGAGCTGCTGCTGACCGACGACGTCAAGGCGAAGGC10.847457627118644No Hit
ACTCGGTCCCGGCGCCGGGCCGGCGCGCGGGCCTGGTGTGGTGGGCCACC10.847457627118644No Hit
GGATCCAGTCGAGGGCGTTCATCGCGGCCCCATCGTCCCCTACCTGTCTC10.847457627118644No Hit
GTTGACGAACAGGCCGACGAGGTTCTCGTGCTCCGCGTGGTCCCGGTTCG10.847457627118644No Hit
GTATTTTACCGGGCAACCCTACCCCCCGGTCACAGTGAGACTACACACGG10.847457627118644No Hit
TCGTTGGGCCACTTGAGCCGCACCTGCGGGCCCAGGGCTTCGGCCGCCGC10.847457627118644No Hit
CGGCCAGCTCGATCGGGCCGAACCGCTCGGTCTCGCCAACCCAGTGGCGC10.847457627118644No Hit
CTTTTATATAACGTTTCAAATGTTTAGATGTCAATGTTTCTTTACTATCA10.847457627118644No Hit
GATCTGAACAGCGGCCAGATCAAGACCGGTGCACCGGCTCGTTCCGAGCG10.847457627118644No Hit
ATATCATACCTTGAGCTACATCTGGATATCTTTGAGCGAATATACGTCCC10.847457627118644No Hit
CATAATCACCTGCTACCTGGGAGATCTGCTCCCGGCTGGTCAGCAAAGCG10.847457627118644No Hit
GCCCGAGCGCGCCAACCTGCAGCAGCTGTGCAAATCCGTGGTGCGTATTT10.847457627118644No Hit
CACCGGGCCCCCCCCCGGGTCAACTGCCTACGTTAGTTCGCGGCAGCTTA10.847457627118644No Hit
GAACAGCCCGCCCCTGGCGACCAGCTCCTGCGATGGCCTGTCTCTTATAC10.847457627118644No Hit
CTCCAGTACTTCCTCGAGCGCTTGAGCGATGCTCCGATCACCGCCTGCGT10.847457627118644No Hit
CGTTGACACCAGGAGCAAGTATTCCTATTATTGTGTTGATAGTTAACACA10.847457627118644No Hit
GTCGGCGACGACAAGCGAGGCGCGAACCCCGATGGCGGCCAGCAGCTCCG10.847457627118644No Hit
CCAAGGGGGCGATGGAGAGGAGGAACAGGGCGGGAACCATAATGAGGGAG10.847457627118644No Hit
CCTATTCGAGCGCCTGGGCCCAAACCCACGGCGGCCTCGACGAAATCGGC10.847457627118644No Hit
AAAGAAATGAGAATGGGCGGGCGCCGTGGCTCACGCCTGTAATCCCAGCA10.847457627118644No Hit
CCAGCGGCACGTAGGCGGCGCCGGCCTTCCACACCCCCAGCAGCGCCGCG10.847457627118644No Hit
CAATGATTCGCTCTCGCTCAATGATTCGGACTCGCTTAAAGATTCGCTCT10.847457627118644No Hit
GAAACAACTACTGTGCCCGTAAGGAATGGCATTGCCAATCCTGCGCCTTT10.847457627118644No Hit
AGCGTGGGCACACCGGCGCGCTGATTGTGCTGGGAGACGGGCCAGAGGTG10.847457627118644No Hit
GTTCCCCGGCTGAAGCCCGAAGACGCCCGCGGCGTCCGCTTTGCGCGTGA10.847457627118644No Hit
GCTTCTTCTTTAGTTGCTGGCTCTTCAGCTATTGCTGCTTCTTCTTTAGT10.847457627118644No Hit
CTCCCAGGTGGCGGACATCGCACCGGCCGCCGACCTGCTCGGCTGGCGAC10.847457627118644No Hit
CCGTCAAACTGGCCTCGCTGCCGTCACGGCACAGTTCTCCAGCTACGACA10.847457627118644No Hit
GGGGATGTCCGCCAGCGCCGGCAGCTCGACGACCTCGCCCAGCAGCACCT10.847457627118644No Hit
AGCCGATTCCAAATCGGAATTGGGGTACTTGAGTACTTTTGCATCTGTCT10.847457627118644No Hit
GTGTTCACCCGCAGCGGCGGCGGCTGGGCGTTCACCCAGGTGCCGCAACT10.847457627118644No Hit
GCCTTCGTGGCAAGGGCAAGCTGTCCGAGGCTGATATTAATGCCACCGCG10.847457627118644No Hit
CCTCTTGTATAAGACGAGAGTCTCGCTCACATCTGTCTCTTATACACATC10.847457627118644No Hit
GTCGTCGCCATGGGCAAGATCGGGCTTCCCCTGGCCGTGCAGTTCGCCGA10.847457627118644No Hit
GCGCCGGGACAGGTGGTCGCTGTGGTGCGCGCGCTTCTTGGTCAGCGCCG10.847457627118644No Hit
ACGCCGACGCTCGACCTGCTCGCCCTGCTGGCGCCCAGCTGTCTCTTATA10.847457627118644No Hit
TAAAACCACTTAATGTGTGGTTTTACACCCCCCTTATATACCTGTCTCTT10.847457627118644No Hit
GGTTGGAGCTGACGATGTGGCCCTGGATGTCGCCGATGGCGATGCCCACC10.847457627118644No Hit
GCGCTGACCGTGACGGCGCTCGGCGTGGCCGAACGGATGGCGGACGCGAC10.847457627118644No Hit
GATGTAGCCCGATTACCGACCGCGCTCACGCGGCGGGACCATCGAGGCGA10.847457627118644No Hit
CGGCTGGATCGACCGGTTTCTGCCCGGGCCGGCTGAAAATAATGCCGCCT10.847457627118644No Hit
CGGATCCTCTTTCCATAACGGCGGTTGAACTTCTCCACCCGGCCGGCGGT10.847457627118644No Hit
GAGCTGGAGCTGGAGCTGGCCTCGTTCTGCGGGATGCAGGCCCTGTCTCT10.847457627118644No Hit
CGATTAGGCCGGTCTGTAGTTTCCCATACTGCAAGTCCAGTGGCGCATAT10.847457627118644No Hit
GTTGGCCGTCCATCGCACGACTTATGAGGTCGTCGGTGCTTCAGGTACTA10.847457627118644No Hit
GGCCAATTACTTCGTCGACCACGGCGGCGGCTGGTCTGTCTCTTATACAC10.847457627118644No Hit
GATCACGTAGCGGCGGTCGCGCTCCAGCACGGTCACCGCCTGCCACAACG10.847457627118644No Hit
CCGCCGGCTACCACGCCTTGGCTAACCAGGTCCCTTCCTTGGCTCGTCGC10.847457627118644No Hit
GCGGTTTCGTCGTCGAAGAGGAAGCCCGCCAGTGCCTTGGTCAGCTCCGT10.847457627118644No Hit
GGAGCAGGTCCTTGTGCATGGCGTCGGGCCACAGGCCTGTCTCTTATACA10.847457627118644No Hit
GCCCAACGTCACCGGCGAGCTGTACGTCGCCCTGTCTCTTATACACATCT10.847457627118644No Hit
CGGCCTGATCATGCCGACGGTGCTGGTCGGCCTGGCCGCGATAGCACTGA10.847457627118644No Hit
AAATAGTTTCGCCCAGGAGTGGAGTAAAATGAAAAAAATAATGACTGTCT10.847457627118644No Hit
GGGAACGTGCTGTCCACCATCAGCCCGCAGGGCCGGCAGGTCGACTACTA10.847457627118644No Hit
GTATTGAATATGGTCTTTTTTTCCCTCTCTGGCTGCCTTCAGGATTTATT10.847457627118644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)