FastQCFastQC Report
Tue 10 May 2022
EGAF00003632002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279671
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC14800.529193230617404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9660.34540585187595424No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT6990.24993653256862527No Hit
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGC6850.24493065065737957No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT5330.19058107562099752No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.15840040619156082No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC4340.15518233924861713No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG4160.14874620536272978No Hit
AGACCTCAACTACCTAACCAACAAACTTAAAATAAAATCCCCACTATGCA3950.1412373824958612No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT3950.1412373824958612No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT3810.1362315005846155No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA3740.13372855962899263No Hit
GGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA3740.13372855962899263No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT3690.1319407446606906No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC3610.12908024071140733No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT3490.12478948478748245No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA3460.1237167958065012No Hit
GGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCC3390.12121385485087835No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG3340.11942603988257632No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG3260.11656553593329304No Hit
GTACATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAA3210.114777720964991No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC3210.114777720964991No Hit
ACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTA3180.11370503198400979No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA3140.11227478000936815No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA3120.11155965402204733No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC3110.11120209102838693No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC3110.11120209102838693No Hit
CAATTACCCACATAGGATGAATAATAGCAGTTCTACCGTACAACCCTAAC3110.11120209102838693No Hit
ATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCC3070.1097718390537453No Hit
TAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG3000.10726889809812244No Hit
CCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCT2970.1061962091171412No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC2970.1061962091171412No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT2960.1058386461234808No Hit
GCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGC2940.10512352013615998No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC2930.10476595714249957No Hit
CTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCT2890.10333570516785795No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTA2870.10262057918053713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)