FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003632035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGCGTCGATCGAGTCGTTCTCCAAGATTCCTCACACTAAAAGTTGT36.8181818181818175No Hit
CAGCGCAATCACAGCGCCCGTGGAACTCATTACCGGAGAAACTGTCTCCA36.8181818181818175No Hit
CCCAACTTACATATATTATTTTCCTAGAAGCAAGTCTGCTGTCCTTCATT36.8181818181818175No Hit
ACAAAAAAATTGGCCGGGCGTGGTGGCGGGCGCCTGTAGTCCCAGCTACT24.545454545454546No Hit
GTGGGGCATCAGCGAGTACGAAATCGCCGACAAGCACGGCCTGGTCCACC24.545454545454546No Hit
AATCGTGTGACCGCGGTGGCTGGCACGAAATTGACCAACCCTGGGGTTAG24.545454545454546No Hit
TCATTCTGGAAAGCCCATCCGGCCATGGCCTTTCGCGCGGATTACAAGGG24.545454545454546No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCTGTACTCTGTG12.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
TCAAGAAGCAATCAAGTTAGAAAAGACTGAGATCATTAATAAGTTGACAA12.272727272727273No Hit
GATGGTAGAGTGCTTCAACAAGATTTCGAGAGACGCTGACTGTCGGGCGG12.272727272727273No Hit
GTGTAGCCGGGTGTCGCGGTCGGCACGACGAAGGCGCTGATGCCCTTCTT12.272727272727273No Hit
GTTGAGGTTGCAGTGAGCTGTGATTGTGCCACAGCTTTCCAGCTTAGGTG12.272727272727273No Hit
GAAGACAGCTATCTGCAAGCTCCAGGAAGAGGGCTCTCACCAGGAACTGA12.272727272727273No Hit
TCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCT12.272727272727273No Hit
GGACGGCACCGGTTCGCCGATCCTGCTGACGAGCGCGGGCGTCTATGGCA12.272727272727273No Hit
CCGTCGACGCACCCGGATCATGGTCAGGAGGACCCGATGACCATTACTGC12.272727272727273No Hit
GCGCAGGCTCTGCCATGGATTTTGGGTTGTGCATGTTCGCCCGCTTGTGC12.272727272727273No Hit
GGTCATGAGCGCGGTGTCGGCCTGCGAGCCGTTGGCCACGTGGTCGACTG12.272727272727273No Hit
TGCCTCTCATTATTATTATTATTATTATTATTATTATTATTATTATTATT12.272727272727273No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT12.272727272727273No Hit
ATGGTCTGGAATTCCTGAGCTCAAGCAACCACCCACTTCTGCCTCCCATA12.272727272727273No Hit
GACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCT12.272727272727273No Hit
CCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGA12.272727272727273No Hit
CCCATTCCCTCTCACCGGAGGGCAGGCGAACCGTCCGCAGGTCGCCCACG12.272727272727273No Hit
GAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAG12.272727272727273No Hit
TCGCAGGCCTGTCCGATGATGATCGGGCCGGCACCGATAACGAGGATCGA12.272727272727273No Hit
ATCAACTATTGTAAGCAGCAACTGAATACCACCTTCTTTGATTAAAACTT12.272727272727273No Hit
CATATTTCACAGTGTTCTTTGTAGTTGTCTTGTTTCTGTTGCTGTGATTG12.272727272727273No Hit
ACTTCGATGTTGCCGGAATCGAAGGCGGCGCTGATCTGTATGTTGGTCAT12.272727272727273No Hit
CTTCTAAGTCATCAATGTTTTTCTCCAATTTAGTTACTGACCTCTCCGCA12.272727272727273No Hit
CTTATATATGCTCGCAGTTTGCCGTTTTATTGTTGGCTTAGGGATGTCTG12.272727272727273No Hit
CCCAGCAGGGCTGAGCTCCTGCTGCCTCGAGCAGTTCCTTCTGTGCTTGT12.272727272727273No Hit
GGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAG12.272727272727273No Hit
ACGCGCAAGAGCGAGGAGTTCGTGGGGCATCCCCGCGGCCTCTTCGTCCT12.272727272727273No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)