FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003632036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCGCTACGGCCGCCGCTGAGTGGTATGAGGACGTAATTTTCCAGCGTG36.8181818181818175No Hit
GGGTTGTCCACAGAACCGAAAATGGTTGGAGGAAATCGGCTCCTCCGACG36.8181818181818175No Hit
GGGTAAATATTAGCTGGTTGCTAAAGACAGGATCTGAGGCTGGGCCAATT36.8181818181818175No Hit
TTTTTGAGATGGAGTCTCGCTCTGTCGCCCAGGCCGGACTGCGGACTGCA24.545454545454546No Hit
CACATCACCCCATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTC24.545454545454546No Hit
GCTGCATGCCTCCCATGTCCCGAATCAAGAGATCACGAAAGAACCTCTTG24.545454545454546No Hit
GCATTAATGGCTGCAGAGGATAAGTACTCGCAGAAGGAAGACAGATATGA12.272727272727273No Hit
CTATAACTAGTGCAACCACAAAAGAAAATTGTCCTTAGCTTGGGCTGCTA12.272727272727273No Hit
GGCCAGACTCTGCGCTAGCAGGCGCTGCGCGCCAACCGGCCGGCCCCTGT12.272727272727273No Hit
ACACAGAGTACAGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA12.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
AATTGGATGTATTCCTAGTTTTGCAGCAGATTCATAGAGAGAAATTTCAT12.272727272727273No Hit
TAATGAAACTGCCCATTTTTTCTTACTGAGGAGTGTGCAACAAATGAAAA12.272727272727273No Hit
GCCGGGTGCAGTGGCTCACTCCTGTATTCCCAGCACTATGGGAGGCAGAA12.272727272727273No Hit
CCCTACCGCGATGGCTACCGCGTTGAGCAGCCACGGCAGGATCGCCCCGA12.272727272727273No Hit
AGCCGCACGTGGGCTCGGAGGCCGCAGGCCTGAAGACCACGGCGGTGCTC12.272727272727273No Hit
AGGCTGGTAGATCTATACCAAGACACTCAGGACACCCAGGACAGCCAATG12.272727272727273No Hit
ATGGCAGAGACCGCGCTCACTCCGAGCACAAGCGGGCGAACAGGCACAAC12.272727272727273No Hit
GAAACAGAGCGAGACTCCATCCCAAAATAATAATAATAATAATAATAATA12.272727272727273No Hit
AATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAG12.272727272727273No Hit
CTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAG12.272727272727273No Hit
AGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAG12.272727272727273No Hit
CCCCGGGGGAGATGGGAGCCATGGAGGTTATAAGCAGAGGGATTCAATCT12.272727272727273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.272727272727273No Hit
ATGAACAGGTGGCCCAGCGCCAGCACCAGTCCGCCGAACAGGACGGCCTT12.272727272727273No Hit
GCCTCACCCTCCCAAAGTGCTGGGATGACAGGTGTGAGCTGCCACACTCG12.272727272727273No Hit
GATGAAGAGGACGAACGCCTCGTCATCACCCTTGTCGGCGACGACGAACA12.272727272727273No Hit
TTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTC12.272727272727273No Hit
GTGGTAACCATCGGACTCGAAGCCTATACGGCGGGCTAGACTCCGGCAAA12.272727272727273No Hit
CCCCCAAGGAGCAGGTCGAAACGCGCGTTTTCGTCTCCATCCGGGATCGC12.272727272727273No Hit
GTCCCATCCCGTCTACCTTTCCAGCTCGAAGCTGCCGCGGAGGGCCGGAG12.272727272727273No Hit
GTCCCATCCCGTCTACCTTTCCAGCTCGAAGCTGCCGCGGCTGCCCGGAG12.272727272727273No Hit
TTCCAGGACGCCACGTAGTTGAGGCTCTCGGCCACCGAATGGTCGCGGGA12.272727272727273No Hit
GAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTC12.272727272727273No Hit
CCATCGATCCGGTCGCGGAAAAAGTGAAAGCCAACAAAGGGCCCACAACA12.272727272727273No Hit
GATCCAAAGAACTAGTAAAACTGGTAACAGGCCTATAAAAAAAGAGTTTC12.272727272727273No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)