FastQCFastQC Report
Tue 10 May 2022
EGAF00003632038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324400
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36211.1162145499383478No Hit
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG22750.7012946979038225No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC18140.5591861898890259No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA16450.5070900123304563No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC15550.47934648581997535No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT15310.4719482120838471No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT15230.4694821208384711No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT14260.43958076448828604No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGT12400.38224414303329224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11550.35604192355117137No Hit
GAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTT11290.34802712700369914No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT11260.3471023427866831No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT11130.343094944512947No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC10780.3323057953144266No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA10100.31134401972872994No Hit
GTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT10070.3104192355117139No Hit
GGAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTT10040.30949445129469794No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGTACAT8370.2580147965474722No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA8140.25092478421701603No Hit
CGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT7580.23366214549938347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6770.2086929716399507No Hit
GTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCG6260.19297163995067818No Hit
GGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTT5850.18033292231812578No Hit
GTACATGGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGT5740.17694204685573367No Hit
GAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAAC5690.17540073982737361No Hit
GTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAG5540.17077681874229347No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC5400.16646115906288533No Hit
GTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGG5110.15752157829839705No Hit
GTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAAC5070.15628853267570902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.1535141800246609No Hit
GTACATGGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACC4940.15228113440197288No Hit
GTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGC4900.15104808877928483No Hit
GAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGT4850.1495067817509248No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC4720.14549938347718866No Hit
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC4690.14457459926017263No Hit
GACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGAC4580.14118372379778052No Hit
CCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCC4530.13964241676942046No Hit
GAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTG4520.13933415536374846No Hit
ATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCA4430.13655980271270038No Hit
AGTCAAAGCTGTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATA4400.13563501849568432No Hit
CCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTC4340.13378545006165227No Hit
ATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTC4230.13039457459926018No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4100.12638717632552404No Hit
CCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCC4060.12515413070283601No Hit
CGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAG3950.1217632552404439No Hit
GCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTT3800.11713933415536376No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA3800.11713933415536376No Hit
ACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGT3770.1162145499383477No Hit
TATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGC3650.11251541307028361No Hit
GGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTG3630.11189889025893958No Hit
TGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTG3590.11066584463625155No Hit
CCTTTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCA3580.11035758323057954No Hit
GAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCC3290.10141800246609124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)