FastQCFastQC Report
Tue 10 May 2022
EGAF00003632147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482849
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG133430.8998218969025168No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG123090.8300912635069383No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG96540.6510440375250616No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA75180.5069970037407721No Hit
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC69980.47192937379328576No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA66470.4482587235787326No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC65030.43854768759327484No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC55830.3765049576861838No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT51490.3472369742300126No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT45600.3075161395394946No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43210.29139851731363076No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG41290.2784504693330204No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA39200.26435597960412693Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCA38820.2617933451079645No Hit
GTACATGGGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCA38300.25828658211321587No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG38160.25734245361462965No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT35090.236639064395633No Hit
TTTGTGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC31320.2112150326837055No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC30900.208382647187947No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA30280.20420150669420825No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC30040.20258300069663196No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG29570.19941342645137838No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT29330.19779492045380212No Hit
GTACATGGGAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAG28540.19246733821178016No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT28350.19118602096369894No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACTCTCCTCATGGACACAC28100.18950007721622364No Hit
GCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCA27550.18579100097177798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27420.18491431022309082No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG27360.18450968372369675No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT26990.1820144869774333No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT26870.18120523397864516No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC26580.1792495392315738No Hit
GTATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGA26440.17830541073298764No Hit
GGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAG26360.17776590873379555No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG25990.1752707119875321Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA25300.17061750724450028No Hit
TTTGCGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC25010.16866181249742893No Hit
GGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATC24880.16778512174874177No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA24840.16751537074914574No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG24090.16245753950671984No Hit
AAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTT23580.15901821426187024No Hit
GGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTGTGTCCATGAG23400.15780433476368802No Hit
CATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAG23390.15773689701378898No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC21740.14660966828045202No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC21460.14472141128327967No Hit
ATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTT20940.14121464828853106No Hit
TCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACT20820.14040539528974294No Hit
GATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTCATGGACACAC20740.13986589329055082No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC20710.13966358004085377No Hit
AATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTT20230.13642656804570122No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC20090.13548243954711506No Hit
CTTATACACATCTCCGAGCCCACGAGACTTACTATTCGGGATCTCGTATG19940.13447087329862986No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC19500.13150361230307334No Hit
GCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATG19340.13042460830468913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.12988510630549704No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT18000.12138794981822155No Hit
CTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCA17950.12105076106872649No Hit
TATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAA17050.11498136357781541No Hit
GAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGG17050.11498136357781541No Hit
ATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC16950.11430698607882529No Hit
GCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAAT16900.11396979732933024No Hit
CTCATGTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGA16410.11066534758427864No Hit
ACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC16410.11066534758427864No Hit
CTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGC16270.10972121908569246No Hit
GACTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCA15820.10668652034023693No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG15780.10641676934064088Clontech Universal Primer Mix Long (96% over 26bp)
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC15510.10459595009336756No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15410.10392157259437745Clontech Universal Primer Mix Long (96% over 26bp)
CTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATG15310.10324719509538732No Hit
CTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCT15150.10216819109700313No Hit
TAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTCTTC15130.1020333155972051No Hit
GGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTTCAGC15070.10162868909781105No Hit
GCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACAC14920.10061712284932585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)