FastQCFastQC Report
Tue 10 May 2022
EGAF00003632196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112876
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA100260.4745190915131792Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT60640.2870021714478275No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG50570.23934201533833505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50350.23830078054746232Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46470.21993718514479788No Hit
GAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAG37520.17757786069793022No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG34580.16366317758354015Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG32780.15514398384003603No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG32340.1530615142582906Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28560.13517120739693195No Hit
GCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAAATCTGCC24760.11718624282731215No Hit
GTACATGGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCT23390.11070218981142292No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG22980.1087617067920692No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21540.10194635179726591Clontech Universal Primer Mix Long (96% over 26bp)
CCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGAC21510.10180436523487418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)