FastQCFastQC Report
Tue 10 May 2022
EGAF00003632221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595578
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA81970.5137323277207382Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG38420.2407904847020954Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35520.22261525290521678Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG29190.18294310901754723Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT26970.1690296557109712No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23360.14640462578451194Clontech Universal Primer Mix Long (96% over 26bp)
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA22080.13838245450864828No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG19960.1250957333329991No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16860.10566703727426674No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA16060.10065318022685196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)