FastQCFastQC Report
Tue 10 May 2022
EGAF00003632239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629339
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG138090.5251890303988949No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG128960.49046547440250193No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG94270.3585311745651664No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC80520.30623666252240583No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA73800.28067890827314396No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72440.27550650562746Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC66470.2528011793078032No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC66240.2519264347427243No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA64820.24652583786267196No Hit
GGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCA53260.20256041537435832No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG49860.18962940876014847No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT48470.1843429089972803No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG43620.16589720838583386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43460.1652886904275181No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT43130.16403362213849185No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT40400.15365078447472919No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT40300.15327046075078185No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC38370.145930212878598No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG37080.1410240368396772No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG37020.1407958426053088No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA36990.1406817454881246No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC35750.13596573131117745No Hit
GTACATGGGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCA35060.13334149761594075No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG34250.1302608754519672Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT33900.12892974241815147No Hit
TTTGTGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC31980.12162752691836237No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT31460.11964984355383615No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC30710.11679741562423103No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30500.1159987358039416Clontech Universal Primer Mix Long (96% over 26bp)
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA30310.11527612072844164No Hit
AAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTT30080.11440137616336274No Hit
GTACATGGGAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAG29800.11333646973631015No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC27790.10569196288496843No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG27410.10424673273396849No Hit
ATTTTTTCCATGAGCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGT27410.10424673273396849No Hit
GGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAG27140.10321985867931065No Hit
GCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCA26870.10219298462465282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)