FastQCFastQC Report
Tue 10 May 2022
EGAF00003632273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109450
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA78630.7087295506782639Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG35070.316102573347154Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31010.2795078642570643Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG26420.2381360133399432Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG26180.23597277930506108Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTACACCGCCCAGATCGGAGCGGACATCGCCCTGCTGGAGCTGGAGG24950.22488620487629007No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15600.1406102122673397No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT14890.13421064491414664Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC14190.12790121231240706Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13600.1225832619766551Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTCCAGTGACCCAGGTGGCCAACCCAGGCCTGACTCACGGCTTTTTG11630.10482671594033079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)