FastQCFastQC Report
Tue 10 May 2022
EGAF00003632274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109450
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70930.6393257920591284Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT37280.33602235341836045Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG33020.2976249492992023Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31700.2857271621073505No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG23630.21298841768443824Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTACACCGCCCAGATCGGAGCGGACATCGCCCTGCTGGAGCTGGAGG22870.20613817657397807No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21430.1931587723646852Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15580.14042994276443282No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13560.12222272297084141Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC13550.122132588219388Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT13420.12096083645049349Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAGAAGGAACAGGGAGCGGCCAGGATGCTGAATCTGCTGCTG11470.10338455991707604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)