FastQCFastQC Report
Tue 10 May 2022
EGAF00003632378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747277
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT137560.7872821538885935No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTG113250.6481513806912127No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAG84640.4844108861960639No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG81310.46535266016779253No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA79210.4533339590688826No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT76270.43650777753040876No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT73250.4192237407119764No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC69520.3978762382839126No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTG67430.38591476909499756No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG62550.3579855970175307No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG61970.35466614623783177No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT57270.3277671485402715No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG54270.31059757554182876No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCA49700.2844425926742011No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCT48810.2793489526846631No Hit
GTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC48270.2762584295449434No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG43260.24758524263754403No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC43160.24701292353759594No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42770.24478087904779838No Hit
TCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTC42750.24466641522780877No Hit
GGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT41890.23974447096825519No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG41770.23905768804831748No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG41450.2372262669284836No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG40290.23058736536908575No Hit
TCGCCACACAGGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC38670.22131579594992665No Hit
CAATAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC38340.21942714292009796No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG37480.21450519866054438No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCAATAGAAAAGTCCACAA36890.21112851597085064No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA36850.2108995883308714No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG36130.20677889081124518No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT35820.2050047016014061No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG35570.20357390385153584No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCC34920.19985382970187326No Hit
GTCTTGAGGCTCTCAATAGAAAAGTCCACAACTTCCAGATGGAATGCTCT33990.194531262072356No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA33360.19092565174268303No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC33360.19092565174268303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31590.18079560367360184Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG31100.1779912400838562No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGTGT30990.1773616890739133No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA30780.1761598189640223No Hit
GGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAA30750.17598812323403787No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT30630.17530134031410016No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT29270.16751780055480614No Hit
CTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT29230.1672888729148269No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA28730.16442727741508645No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC27150.15538463563590663No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG26960.15429722934600523No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC25940.14845957452653472No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA25600.1465136895867112No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG25460.1457124428467839No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTATTGAGAG25460.1457124428467839No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAA25040.1433087026270019No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTGCCCAC24800.1419351367871265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.13976032420732373No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT24370.1394741646573497No Hit
CATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAG24140.13815783072746907No Hit
CCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAG23770.13604025005766116No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAGGA23700.1356396266876975No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA23680.13552516286770788No Hit
CTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTTT23540.13472391612778054No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC23180.13266356736796742No Hit
GCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTT23020.13174785680805046No Hit
CCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGC22860.13083214624813352No Hit
CATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCAATAGAA22750.13020259523819064No Hit
GCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCAC22480.1286573336683308No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC22470.12860010175833597No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCAT22190.12699760827848133No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT21850.1250517233386578No Hit
GAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGAT21850.1250517233386578No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT21640.12384985322876682No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC21220.12144611300898484No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTGCCCACTCCCA21200.12133164918899522No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAACACCCTGTGTG20910.11967192379914576No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC20880.11950022806916133No Hit
CTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGGAGACCCATGAGCA20660.11824112604927553No Hit
CTGCAGGCCAAGCCCGCAGTGCCTACGTCTAAGCTGGGCCAGGCAGAGGG20300.11618077728946241No Hit
AGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCAGAGAGC19900.11389150088967004No Hit
GGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGC19340.11068651392996073No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT19320.1105720501099711No Hit
GGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACC19240.11011419483001265No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC19100.10931294809008532No Hit
GACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGGAAGTT18990.10868339708014242No Hit
CCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACTGACCCTGC18990.10868339708014242No Hit
CAATAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGTGCCTACGTCTA18980.1086261651701476No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG18740.10725259933027219No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG18710.10708090360028776Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTGCCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC18650.10673751214031892No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG18610.10650858450033968No Hit
GGGCGCCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC18600.10645135259034486No Hit
GTCTTGAGGCTCTCAATAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCA18500.10587903349039676No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG18350.10502055484047464No Hit
CCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCT18120.10370422091059402No Hit
GCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATC18090.10353252518060961No Hit
AGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCC18080.10347529327061479No Hit
GCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGCACTGCGG17730.10147217642079648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)