FastQCFastQC Report
Tue 10 May 2022
EGAF00003632392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566738
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98761.742604166299066No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28030.49458479932526145Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG19190.3386044345005982Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16330.2881401988220306No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15730.2775532962321214Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG15610.2754359157141395No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT12000.2117380517981854No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11630.20520946186774136Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9750.17203716708602565Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.15792129696614662No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA8800.15527457131866929No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT8590.15156915541220103No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC8080.1425702882107782No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC7690.13568880152733714Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT7170.12651348594941578Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6840.12069068952496567Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC6700.11822041225398686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6520.11504434147701406No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA6290.11098602881754885No Hit
GAGTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAAC5990.1056925775225942No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5830.1028694034986184No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT5720.10092847135713505Clontech Universal Primer Mix Long (96% over 26bp)
GAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTT5680.10022267785114108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)