FastQCFastQC Report
Tue 10 May 2022
EGAF00003632580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285706
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT53480.41595823617529976No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC30090.2340348415578678No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA30070.23387928499983665No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT28300.2201125296140797No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG27650.2150569414780673No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG27160.21124580580630406No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTG26120.2031568647886842No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAG25990.2021457471614817No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT23910.18596786512624192No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTG23570.18332340363971233No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG22750.17694558476043512No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG22720.17671224992338838No Hit
GTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC21360.16613440397727006No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG20310.1579676846806346No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCA19250.14972318710498356No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC17670.13743421902052258No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG17620.1370453276254447No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCT17370.1351008706500553No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT17070.13276752227958802No Hit
GGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT17000.132223074326479No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16840.13097862186222978No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT16590.12903416488684039No Hit
TCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTC16530.1285674952127469No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCAATAGAAAAGTCCACAA15930.12390079847181237No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG15650.12172300665937626No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA15640.12164522838036067No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG15510.12063411075315818No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC15060.11713408819745727No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG14950.11627852712828594No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT14830.11534518778009903No Hit
TCGCCACACAGGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC14120.10982292996999314No Hit
GTCTTGAGGCTCTCAATAGAAAAGTCCACAACTTCCAGATGGAATGCTCT14110.10974515169097755No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13670.10632290741429222No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG13280.10328955453268478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)