FastQCFastQC Report
Tue 10 May 2022
EGAF00003632698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115072
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG57210.5130610400045916No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG47860.4292099523618206No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC47820.4288512311312632No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG44150.39593855822763013No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA35460.3180063708890547No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT28580.2563063192331975No Hit
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT27160.24357171554841303No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT25040.22455949032887562No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC24890.2232142857142857No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23120.20734087126212478No Hit
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA23070.20689246972392814No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA21740.19496498880789762No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC21660.19424754634678298No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG21620.19388882511622568No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG19390.17389011651265568No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG18770.1683299374390174No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC18290.1640252826723297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17530.15720957929174081No Hit
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC16960.15209780175629914No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC16800.1506629168340699No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA16700.14976611375767662No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG16580.14868995006600472No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT15890.1425020088388911No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC15800.1416948860701372No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG15690.14070840268610457No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT15400.13810767376456407No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG15220.13649342822705618No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT15030.13478950238190898No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA14990.13443078115135165No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA14460.1296777248464673No Hit
GGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCA13600.12196521838948517No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG13410.12026129254433796No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC13040.11694312116168283No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC13040.11694312116168283No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA13030.11685344085404352No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT12940.11604631808528956No Hit
ATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTTT12600.11299718762555243No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT12550.11254878608735579No Hit
CTCAGAGACGGAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATG12270.11003773747345463No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC12120.10869253285886472No Hit
CCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACAT11940.10707828732135682No Hit
GTACATGGGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCA11580.10384979624634104No Hit
CTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCT11580.10384979624634104No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC11570.10376011593870173No Hit
AAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTT11520.10331171440050507No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG11510.10322203409286576No Hit
GGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTGTGTCCATGAG11450.10268395224702978No Hit
AATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCTT11170.10017290363312863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)