FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003632861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003632861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2729
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA461.6855991205569805Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT421.5390252839868084Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTCACTGGCTCGGATCTCGTATG371.3558079882740932No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG351.282521069989007Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACATCTCCGAGCCCACGAGACTCACTGGCTCGGATCTCGTATGCCGTC281.0260168559912055No Hit
ATACACATCTCCGAGCCCACGAGACTCACTGGCTCGGATCTCGTATGCCG281.0260168559912055No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG180.659582264565775Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTCACTGGCTCGGATCGCGTATG150.5496518871381458No Hit
ATACACATCTCCGAGCCCACGAGACTCACTGGCTCGGATCGCGTATGCCG120.4397215097105167No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT120.4397215097105167Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC120.4397215097105167Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC110.40307805056797363Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC110.40307805056797363Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT110.40307805056797363Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG110.40307805056797363Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACTCACTGGCTCGGATCGCGTATGCCGTC110.40307805056797363No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT100.36643459142543056Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG100.36643459142543056Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC80.29314767314034446Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT80.29314767314034446Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT70.2565042139978014Clontech Universal Primer Mix Long (100% over 23bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC70.2565042139978014Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG60.21986075485525836Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT60.21986075485525836Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC50.18321729571271528Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG50.18321729571271528Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC50.18321729571271528Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG50.18321729571271528Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50.18321729571271528Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACTCACTGGCTCGGATCTCGTATGCCGTCT50.18321729571271528No Hit
CATCTCCGAGCCCACGAGACTCACTGGCTCGGATCTCGTATGCCGTCTTC50.18321729571271528Illumina PCR Primer Index 9 (95% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG40.14657383657017223Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA40.14657383657017223No Hit
ATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCA40.14657383657017223No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT40.14657383657017223Clontech SMART CDS Primer II A (100% over 26bp)
ACATCTCCGAGCCCACGAGACTCACTGGCTCGGATCTCGTATGCCGTCTT40.14657383657017223Illumina PCR Primer Index 9 (95% over 21bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT40.14657383657017223Clontech Universal Primer Mix Long (96% over 26bp)
GCCGTGGTGCGGCCTGGCTTCCTGAGCACGGCAGAGGAGGAGACGCTGAG40.14657383657017223No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA40.14657383657017223Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG40.14657383657017223Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30.10993037742762918Clontech Universal Primer Mix Long (96% over 26bp)
GGTTATAGGTGTGAGCCACTGCTCCCAGCCCCGATCATCATTACAAAGAT30.10993037742762918No Hit
CAATATACAAGTTTACTTGGGAAAATGAATTCAGAGGAGGAAGATGAAGT30.10993037742762918No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA30.10993037742762918Clontech Universal Primer Mix Long (95% over 21bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG30.10993037742762918Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT30.10993037742762918Clontech Universal Primer Mix Long (96% over 25bp)
GGCCGGGTCGGCTGCGTTTACCTGTTGCCAGTACTCCTCCAGAGACGGCA30.10993037742762918No Hit
GTACATGGGAGCAGTAGCACCAGCGGCTGCGGCGGCGGAGCTCCTCCGAG30.10993037742762918No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA30.10993037742762918Clontech Universal Primer Mix Long (96% over 26bp)
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT30.10993037742762918No Hit
GTATCAACGCAGAGTACATGGGTCCGCCTGACTCAGAAGGCCGACTACGC30.10993037742762918No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA30.10993037742762918Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG30.10993037742762918No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCT30.10993037742762918Clontech Universal Primer Mix Long (96% over 25bp)
CGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATC30.10993037742762918No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT30.10993037742762918Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT30.10993037742762918Clontech Universal Primer Mix Long (95% over 21bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA30.10993037742762918Clontech Universal Primer Mix Long (96% over 26bp)
GGCCTTTCCTGGGTCAGCATAGGGCACCCAGGTGGGGGCACAGGTGGACA30.10993037742762918No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.10993037742762918Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA30.10993037742762918No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG30.10993037742762918Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAACAGGAAGAAAAGTATGAAATTCCTGA30.10993037742762918No Hit
CAAGAAGGCCACCGTGTATGAGGTGGTCTACTGAGACATGACCCCCCTTC30.10993037742762918No Hit
ACCTCGGGCCGATCGCACGCCCCCCCGTGGCGGCGACGACCCATTCGAAC30.10993037742762918No Hit
CCACAGGACAGCAGAACCCTCAGCCAGCAGCCACAGGGCCTGCCAGCCAG30.10993037742762918No Hit
AATCACAGCCGGGCAAGGGTGCTCCGAGCCTCGCATCCCCCGGCCGGGGG30.10993037742762918No Hit
CCGCTACCATGGCCGAAGACGCAGACATGCGCAATGAGCTGGAGGAGATG30.10993037742762918No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT30.10993037742762918Clontech Universal Primer Mix Long (96% over 26bp)
CGTGTGTGGTGCACTACACAGGAATGCTCCAAAATGGGAAGAAGTTTGAT30.10993037742762918No Hit
GTGAAGCCCCTTTGGGTTTAAGAGCATTTTCCTGCTTCCTTTGTTCTTCC30.10993037742762918No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC30.10993037742762918Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30.10993037742762918Clontech Universal Primer Mix Long (96% over 26bp)
AGAGACCATCATTATCATTGCTGGTTCAGCTGGGGGTTCCTTCCTTCACG30.10993037742762918No Hit
ACCTCCCCAATCACAAGTATTATAAGTGGAAGTAAAAAATCACATTTTAC30.10993037742762918No Hit
AAATAGGATTATCCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGT30.10993037742762918No Hit
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC30.10993037742762918No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCT30.10993037742762918Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.10993037742762918No Hit

[FAIL]Adapter Content

Adapter graph