FastQCFastQC Report
Tue 10 May 2022
EGAF00003633080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721526
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT117170.6806170804274811No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92110.5350485557580891No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG91380.53080813185511No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72930.42363577430721344Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAG69660.4046409987418139No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG69530.40388585475909167No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT64900.3769911113744434No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC63880.37106613551000683No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT63780.37048525552329736No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTG62500.3630499916934162No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG57730.33534201632737465No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG54550.31687003275001363No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA53920.313210488833744No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTG51820.3010120091128452No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG48720.2830047295248518No Hit
TCGCCACACAGGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC45740.2656945059209097No Hit
GTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC44280.2572136581149515No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCA42800.2486166343116514No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG41930.2435629784272791No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT41330.24007769850702226No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG40770.23682477058144924No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39030.22671745881270455Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT38030.22090865894560988No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG35920.20865209122604014No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT35490.20615430728318945No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA34870.20255285136559076No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG34830.20232049937090696No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCT33660.19552420352640623No Hit
GTCTTGAGGCTCTCAATAGAAAAGTCCACAACTTCCAGATGGAATGCTCT33450.19430435555431635No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32870.19093525163140143Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32370.1880308516978541No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG31980.1857654197496872No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC31280.18169925984272095No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC30800.1789110359065155No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCAATAGAAAAGTCCACAA30790.17885294790784456No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC29990.17420590801416883No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT29940.1739154680208141No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT29210.169675044117835No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG29090.16897798813378362No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCC28970.16828093214973228No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG27960.16241404428396666No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG27540.1599743483397869No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG27050.15712803640491052Clontech Universal Primer Mix Long (96% over 26bp)
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG26880.15614054042750444No Hit
CTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT26730.15526922044744024No Hit
GGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAA26440.1535846684859828No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTATTGAGAG26330.15294570050060238No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA26240.15242290851256385No Hit
CAATAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC26100.15160967653117058No Hit
TCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTC26100.15160967653117058No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT25240.1466141086454692No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA24750.1437677967105928No Hit
GGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCA24710.143535444715909No Hit
CATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAG24620.1430126527278705No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGTGT24250.14086339677704549No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC23350.13563547689666028No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCAC23240.13499650891127987No Hit
GGGCGCCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC23080.13406710093254473No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG22650.131569316989694Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22450.1304075570162751No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA22390.13005902902424943No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC22300.1295362370362109No Hit
CCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGC22130.12854874105880482No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC22120.12849065306013385No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT21390.12425022915715477No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA20860.12117156522759459No Hit
CAATAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGTGCCTACGTCTA19970.11600173334588035No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAGGA19840.11524658936315803No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC19640.11408482938973911No Hit
GACCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGT19520.11338777340568774No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC19360.11245836542695259No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAA19350.11240027742828165No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCAT18560.10781132553327687No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT18360.10664956555985793Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC18240.10595250957580657No Hit
GGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCT18210.10577824557979373No Hit
CCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGT18180.1056039815837809No Hit
CATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCAATAGAA18070.10496501359840049No Hit
GCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTC18050.10484883760105859No Hit
GGGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG18030.10473266160371669No Hit
GGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAGGCCAGT17770.1032223736382721No Hit
CCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACTGACCCTGC17710.10287384564624641No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG17660.10258340565289167No Hit
CTTCCAGATGGAATGCTCTCTGCAGGCCAAGCCCGCAGTGCCTACGTCTA17620.10235105365820789No Hit
GCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTT17550.10194443766751128No Hit
GCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCG17520.10177017367149843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)