FastQCFastQC Report
Tue 10 May 2022
EGAF00003633093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156618
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA58673.746057285880295Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30711.9608218723262971Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACAGAAGCCTCAGCATCTCGTATG29521.8848408228939202No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28671.8305686447279368Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG22781.4544943748483572Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG18721.1952649120790713Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACAGAAGCCTCAGCATCTCGTATGCCG16491.0528802564200794No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA14640.9347584568823506No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14470.923904021249154No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12990.829406581618971Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT12290.7847118466587493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11710.7476790662631371Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10760.6870219259599791Clontech SMART CDS Primer II A (100% over 26bp)
ACACATCTCCGAGCCCACGAGACAGAAGCCTCAGCATCTCGTATGCCGTC10520.6716980168307602RNA PCR Primer, Index 40 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.6550971152741064No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC8650.5522992248655966Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT8050.5139894520425494Clontech SMART CDS Primer II A (100% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6750.4309849442592805Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.42459998212210603No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT6620.42268449348095366Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA6310.40289111085571266No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6070.3875672017264937No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA6030.38501321687162393No Hit
CTTATACACATCTCCGAGCCCACGAGACAGAAGCCTCAGCATCGCGTATG5880.37543577366586217No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5390.344149459193707Clontech Universal Primer Mix Long (100% over 23bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG5340.3409569781251197Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA5220.33329502356051033Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC5160.3294640462782056Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT4960.3166941220038565Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4500.2873232961728537Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG4450.2841308151042664Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA4270.2726378832573523No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC3890.24837502713608905Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3810.24326705742634944Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA3790.24199006499891454No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG3750.23943608014404474Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA3650.2330511180068702Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3630.2317741255794353Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACAGAAGCCTCAGCATCGCGTATGCCG3520.22475066722854334No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC3480.22219668237367352Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT3410.21772720887765135Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3410.21772720887765135Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA3400.2170887126639339No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG3300.21070375052675938Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA3210.20495728460330231No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA3150.2011263073209976Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT3140.20048781110728014Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3080.19665683382497542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3040.1941028489701056No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2960.18899487926036598Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAA2950.18835638304664853No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA2880.18388690955062637No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2870.18324841333690892Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2860.18260991712319147Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA2770.1768634511997344No Hit
CACATCTCCGAGCCCACGAGACAGAAGCCTCAGCATCTCGTATGCCGTCT2720.17367097013114713RNA PCR Primer, Index 40 (95% over 23bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2660.1698399928488424Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACAGAAGCCTCAGCATCGCGTATGCCGTC2640.1685630004214075No Hit
CATCTCCGAGCCCACGAGACAGAAGCCTCAGCATCTCGTATGCCGTCTTC2640.1685630004214075RNA PCR Primer, Index 40 (96% over 25bp)
ACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2420.15451608371962353No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT2360.15068510643731883Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2340.1494081140098839No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT2320.148131121582449Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA2310.14749262536873156No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA2210.14110766323155705No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2190.13983067080412215No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.13727668594925233No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.13663818973553488No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT2090.1334457086669476Clontech Universal Primer Mix Long (96% over 25bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1950.12450676167490325Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1930.12322976924746835No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1910.12195277682003344Clontech Universal Primer Mix Long (96% over 25bp)
GGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.11939879196516363No Hit
TTATACACATCTCCGAGCCCACGAGACAGAAGCCTCAGCATCTCGTATGC1840.11748330332401129No Hit
GTATAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1830.11684480711029382Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1780.11365232604170657No Hit
CATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.11365232604170657No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1770.11301382982798912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1760.11237533361427167No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAA1740.11109834118683676No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.10599037147709714Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG1630.10407488283594478No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT1600.10215939419479243No Hit
GTACATGGGAAGCAGTGGTATCAACTCAAAAAAAAAAAAAAAAAAAAAAA1570.10024390555364007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)