FastQCFastQC Report
Tue 10 May 2022
EGAF00003633109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11756
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCTCGTATG2131.8118407621640014No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1861.5821708063967335Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCTCGTATGCCG1711.4545763865260293No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCGCGTATG1441.2249064307587616No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1411.1993875467846205Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCGCGTATGCCG1120.9527050017012589No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1050.8931609390949302Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCTCGTATGCCGTC860.7315413405920381No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG700.5954406260632868Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT660.5614154474310991Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG640.5444028581150051Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCGCGTATGCCGTC610.5188839741408643No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT450.38278325961211296Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC410.34875808097992517Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC370.3147329023477373Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG320.27220142905750255Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC310.26369513439945563Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG310.26369513439945563Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCGCGGATG310.26369513439945563No Hit
CATCTCCGAGCCCACGAGACCTCCTCCACCCAATCTCGTATGCCGTCTTC310.26369513439945563Illumina PCR Primer Index 4 (95% over 22bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA300.25518883974140866Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG270.22966995576726776Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC260.22116366110922084Clontech SMART CDS Primer II A (96% over 26bp)
CACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCGCGTATGCCGTCT250.21265736645117386No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA240.2041510717931269Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCTCGGATGCCG220.187138482477033No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC220.187138482477033Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG220.187138482477033No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC210.17863218781898604Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC200.1701258931609391Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC200.1701258931609391Clontech Universal Primer Mix Long (96% over 26bp)
CACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCTCGTATGCCGTCT200.1701258931609391No Hit
ATACACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCGCGGATGCCG200.1701258931609391No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT200.1701258931609391Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG190.16161959850289215Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCTCGGATG190.16161959850289215No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG180.1531133038448452No Hit
TCTCCGAGCCCACGAGACCTCCTCCACCCAATCTCGTATGCCGTCTTCTG170.14460700918679822RNA PCR Primer, Index 32 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCTCGTTTGCCG170.14460700918679822No Hit
TTATACACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCTCGTATGC160.13610071452875128No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC160.13610071452875128Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCTCCTCCACCCAATCTCGTTTG150.12759441987070433No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT150.12759441987070433Clontech Universal Primer Mix Long (100% over 23bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT150.12759441987070433Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA140.11908812521265737Clontech Universal Primer Mix Long (96% over 26bp)
GCTGGACATGTCCTACGAGCAGCTGATGCAGCTGTACAGTGCGCGCCAGC140.11908812521265737No Hit
GTATCAACGCAGAGTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCA140.11908812521265737No Hit
CATCTCCGAGCCCACGAGACCTCCTCCACCCAATCGCGTATGCCGTCTTC130.11058183055461042RNA PCR Primer, Index 4 (95% over 21bp)
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC130.11058183055461042No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC130.11058183055461042No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA130.11058183055461042Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT120.10207553589656344No Hit
GTATAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG120.10207553589656344Clontech Universal Primer Mix Long (96% over 26bp)
CTGATAATGCGTGGGTGCTTTTGGGATAGAGCAGGAGCCGAACAGGCACA120.10207553589656344No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA120.10207553589656344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)