FastQCFastQC Report
Tue 10 May 2022
EGAF00003633127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598979
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA44470.742430035109745Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG28470.47530881717055184Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23060.3849884553548622Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG18240.30451818845068024Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG16900.28214678644827285Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTCCGCCTCAGACATCTCGTATG16270.2716288884919171No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11560.19299507996106707Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11250.1878196063634952Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACTCCGCCTCAGACATCGCGTATG9830.16411259827139182No Hit
ATACACATCTCCGAGCCCACGAGACTCCGCCTCAGACATCTCGTATGCCG9330.15576506021079203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7730.1290529384168727No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT7710.12871903689444872Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACTCCGCCTCAGACATCGCGTATGCCG7050.11770028665445699No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6910.11536297599748906Clontech SMART CDS Primer II A (96% over 26bp)
GTTTTGGGGGGGCTTAACTACCTGTTCAACTCTGACTGAATCATCGACAA6640.11085530544476517No Hit
GAGACGCCGCCGCTGCGAAGGACCAATGAGAGCCCCGCTGCTACCGCCGG6640.11085530544476517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)