FastQCFastQC Report
Tue 10 May 2022
EGAF00003633131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169299
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4950.292382116846526Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3450.20378147537788174Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.1500304195535709No Hit
CATCTCCGAGCCCACGAGACTCCACGCCTTGGATCTCGTATGCCGTCTTC1940.1145901629661132RNA PCR Primer, Index 8 (95% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1730.10218607316050302Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1700.10041406033113012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)