FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003633323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA675.682782018659881Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG403.3927056827820183Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACGTTGGGCTCATTATCTCGTATGCCG282.374893977947413No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT262.205258693808312Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACGTTGGGCTCATTATCTCGTATGCCGTC211.7811704834605597No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG201.6963528413910092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC151.2722646310432568Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG121.0178117048346056Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACGTTGGGCTCATTATCGCGTATGCCG121.0178117048346056No Hit
CACATCTCCGAGCCCACGAGACGTTGGGCTCATTATCTCGTATGCCGTCT100.8481764206955046No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC100.8481764206955046Clontech Universal Primer Mix Long (96% over 25bp)
ACACATCTCCGAGCCCACGAGACGTTGGGCTCATTATCGCGTATGCCGTC90.7633587786259541No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA80.6785411365564037No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT80.6785411365564037Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA80.6785411365564037Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC80.6785411365564037Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG70.5937234944868532Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG70.5937234944868532Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT60.5089058524173028Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50.4240882103477523Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA50.4240882103477523No Hit
GCCCAGATGCGAGGTGGAAAAGTCACACTAGAGCGACACCAACATCGTTA40.33927056827820185No Hit
GGTCTGGTCTTCTCACTGAGCGTCGCAATCCCTGCAGCCTATGCCATCGC40.33927056827820185No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG40.33927056827820185Clontech Universal Primer Mix Long (96% over 26bp)
GGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGG40.33927056827820185No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC40.33927056827820185Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG40.33927056827820185Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC40.33927056827820185No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG40.33927056827820185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC40.33927056827820185No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC40.33927056827820185Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT30.2544529262086514No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA30.2544529262086514No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.2544529262086514Clontech Universal Primer Mix Long (100% over 23bp)
CTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAA30.2544529262086514No Hit
CACATCTCCGAGCCCACGAGACGTTGGGCTCATTATCGCGTATGCCGTCT30.2544529262086514No Hit
GCTATGTCCTCATGATGACCCAGAGTCCACATCGATCCAGGTTCCCTTGC30.2544529262086514No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC30.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC30.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
CACATAAATAGAGTCAACTGATCTTTGACAAAGGAGCAAAGGCAATACAA30.2544529262086514No Hit
TCCAACCGACAGATCGAACTCAGATCTCCTGTGTTACAGGCAGATTCTTT30.2544529262086514No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT30.2544529262086514No Hit
ACCTTGCGGAACAGGTAGCGCGCCACCTCGTCGGGGCTCAGGCGGCGCTG30.2544529262086514No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA30.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GATCTTAAATCCTGATCTTCATATCGCGACTCTTGGTGAGAATGCGAGTT30.2544529262086514No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT30.2544529262086514Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT30.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.2544529262086514Clontech SMART CDS Primer II A (96% over 26bp)
ACATAGGGTAACGAAGCGAACGTGGCGAACTGAAACATCTAAGTAGCTAC30.2544529262086514No Hit
ATCAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT20.16963528413910092No Hit
GGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATCTC20.16963528413910092No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG20.16963528413910092No Hit
CTGCCATGTTGTGTCCAGTCGCTATCGGAGTAGCCGCGCTGGTGCCACAC20.16963528413910092No Hit
GACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGTCCTGCC20.16963528413910092No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT20.16963528413910092No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTGGGCTCATTATCGCGTATGC20.16963528413910092No Hit
CTCAATATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCT20.16963528413910092No Hit
CTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTG20.16963528413910092No Hit
GCATCAAAAATAGCAGTTGTTGGACATGTTAAGACACACTGCCCCAATCT20.16963528413910092No Hit
CTAATTAGGTGGACTTTCCAACAATGCCTATAACAGAATCATCTGACAGG20.16963528413910092No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTATGTTTTGCTGGTAGATACCGGCGCGCTAGCAAACCCATAGTTCT20.16963528413910092No Hit
GGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTT20.16963528413910092No Hit
CAGGTATCTGGCGTGTACACCCGAATCGGCGGGGCTCGGCGACACAAAAT20.16963528413910092No Hit
ATGGAGTATATATTGAAGATACATTTGCAGAAGCATTCCCAATATGGGTT20.16963528413910092No Hit
CTCCTCCGGCACGCCCAGCGCCTGTGCGCGGGCGACCAGCGAGAGGTCTA20.16963528413910092No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTAT20.16963528413910092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG20.16963528413910092No Hit
TACCTCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA20.16963528413910092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA20.16963528413910092No Hit
GCTCTCTGACACCCAATGGGAGTTGATCGCCCCGATGCTCCCGACCCGGA20.16963528413910092No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
CTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGG20.16963528413910092No Hit
TATCTGAACCGCAGCCTAGACCGTTTGTAAAATATGCCCTACCAGATGAC20.16963528413910092No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG20.16963528413910092No Hit
CCCGCGCGCCGGATCCATTCGGCCTGCCAGCGCGCACGTCGGGCCGACTG20.16963528413910092No Hit
CTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC20.16963528413910092No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA20.16963528413910092No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC20.16963528413910092No Hit
GCTTGTTCCGTTAGCTGGCATAAGATTCCATGCCTAGATGTGATACACGT20.16963528413910092No Hit
GTACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCA20.16963528413910092No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.16963528413910092Clontech Universal Primer Mix Long (96% over 25bp)
CCTTCATACTCCCTGTGAATGTGTTATTAATGGGAAAGAACAGAACCACT20.16963528413910092No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT20.16963528413910092No Hit
GTACATGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAA20.16963528413910092No Hit
CAGGTCGGCCGCGAGGTCCTGGCCTACGTCGTCGACATCGCCCGCGCGAC20.16963528413910092No Hit
CAGAGTACATGGGGGAATTCCAGCAGCGATTAAGGCAGAGGCGTTTGTAT20.16963528413910092No Hit
ATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGA20.16963528413910092No Hit
GTACATGGGTGATGTGCTTGGAGGACAGCGTGGCGCCCGTGATGTTGGCG20.16963528413910092No Hit
ATGTGTGAGTGCTCCGCCCTAGTGCTGATGTATGCCCCATAGTGCTCAGT20.16963528413910092No Hit
GTATCAACGCAGAGTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTC20.16963528413910092No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG20.16963528413910092No Hit
GAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA20.16963528413910092No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA20.16963528413910092No Hit
CCATCACCCTCGACGGGCAGGCCCTCCCCCAGGAGGGCAAGGAGCTCGCC20.16963528413910092No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT20.16963528413910092No Hit
TCCCACTTGGGATCATAGACCGGCTCTTCGGTCTCTGTGGCAATGGGTTC20.16963528413910092No Hit
AGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTAT20.16963528413910092No Hit
ATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTT20.16963528413910092No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTACGTTGAT20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA20.16963528413910092No Hit
CAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAA20.16963528413910092No Hit
AAGTTAAGCTCTGCACCTGTTACACTATAGTCATCGTCCATAACACGATC20.16963528413910092No Hit
CGTATCTTCCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGC20.16963528413910092No Hit
GTACATGGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.16963528413910092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGC20.16963528413910092No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG20.16963528413910092No Hit
ACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16963528413910092No Hit
GTACTTGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCA20.16963528413910092No Hit
AAGTAGGGCGGGACACGTGAAATCCTGTCTGAACATGGGGGGACCATCCT20.16963528413910092No Hit
GGCAGCAAGAGCTGGACAACCTGCTGCATGAGAAGCGGTACCTGCGGGCG20.16963528413910092No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC20.16963528413910092No Hit
CTTCAAAACGGTGGTTCAATTCTCTTAGTTGAGTACACACATCTCAAATA20.16963528413910092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.16963528413910092No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA20.16963528413910092No Hit
GTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACT20.16963528413910092No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAAACAAAACCGAGCC20.16963528413910092No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG20.16963528413910092No Hit
CACTTAGCCAATCTTTGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTC20.16963528413910092No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT20.16963528413910092No Hit
GTACTTGGGGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGCAGAAAGA20.16963528413910092No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT20.16963528413910092No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCAACGCAGAGTACAT20.16963528413910092No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTGGGCTCATTATCTCGTATG20.16963528413910092No Hit
GGCCGATCCGGGTCGCCGCCATGTGCTTCGATATTCTCGACTACGACCAC20.16963528413910092No Hit
AATCTCCCCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATA20.16963528413910092No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACGTTGGGCTCATTATCGCGTATGC20.16963528413910092No Hit
GATTGAGAATGTTGTGGACAACGTGCCTATTCCGCAGGTGTCCTCCGTCA20.16963528413910092No Hit
GTATCACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20.16963528413910092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG20.16963528413910092No Hit
GCACCGGCAGCACGACGCGCTCGTTCGCCAGCAAGGCTTTGAGGTAGCGC20.16963528413910092No Hit
CTAATGATTTCGTTGGAAACGGGAATATCATCATCTAAAATCTAGACAGA20.16963528413910092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)