FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003633324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13511.450381679389313No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT524.410517387616624Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA433.64715860899067Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC201.6963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG171.441899915182358Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG161.3570822731128074Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT131.102629346904156Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG110.9329940627650551Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC110.9329940627650551Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC100.8481764206955046Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG80.6785411365564037Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC70.5937234944868532Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT70.5937234944868532Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC60.5089058524173028Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG50.4240882103477523Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA50.4240882103477523Clontech Universal Primer Mix Long (96% over 26bp)
ATTGTGCGCAGTCTGCTCGACCCATCCAGTGCTGCGGCCTCCTCGTAGAT40.33927056827820185No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC40.33927056827820185Clontech Universal Primer Mix Long (96% over 26bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA40.33927056827820185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC40.33927056827820185Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40.33927056827820185Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA40.33927056827820185Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT40.33927056827820185No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC40.33927056827820185No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC40.33927056827820185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA40.33927056827820185Clontech SMART CDS Primer II A (96% over 26bp)
GCTCAGCGCAGGTGGAGCCCCGGCCACTGCGAGGTCGGCACGGTCGGGAT30.2544529262086514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG30.2544529262086514No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT30.2544529262086514No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG30.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT30.2544529262086514No Hit
CATATGGGGAGGCAGATGGTTCAGGAAAGCCTGAATCACATCAACAAGGC30.2544529262086514No Hit
GTCCGAGTCCAAGTTCTTCTAGTCTCGCTTTCACTTCTTCAAGTGATTTG30.2544529262086514No Hit
GTATAAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC30.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG30.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
ATAATAAATAATGTGATTGTGATGATAATAAACACTGAGGTACATTTCCT30.2544529262086514No Hit
ATGTCATGTTGTTCTTCCCCTGTGGTAGTCACCATAAGAAGTATAGTTGT30.2544529262086514No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG30.2544529262086514No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
TCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTA30.2544529262086514No Hit
CCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTG30.2544529262086514No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.2544529262086514Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC30.2544529262086514No Hit
AACGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCA30.2544529262086514No Hit
GTCTGTGACTTGTCTTCATATTCTCTTGTCTTTTACAGAGCAGAAATTTT30.2544529262086514No Hit
GCTCTACCCCCGATGGTGATACTTGAGGCACTACCTAAATAGTTTTCGGA30.2544529262086514No Hit
GTAGAAGAGCTGAAGGCACTCGTGGAAGAGCTTGAAGCTGATCTCCTCAT30.2544529262086514No Hit
GGTTCACCCAAAAGGACAGAGAAAATCATCTGAAAGAGGAGCTGTCCCTC30.2544529262086514No Hit
CCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG30.2544529262086514Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30.2544529262086514Clontech SMART CDS Primer II A (96% over 26bp)
ATATTTGGTCCGGGTTATTATCGTCGCGGCTACCGTAATACTTCAGATCA30.2544529262086514No Hit
TAATAGGGCTGCGTGCGTCCTAAATACACTCGCTGTTGAGATACTAAAAT20.16963528413910092No Hit
GTTATAATGTGTCCAGCAGAAGCAGGAATTGAGAAATATGTCCCTCCATC20.16963528413910092No Hit
GCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCAC20.16963528413910092No Hit
ACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGA20.16963528413910092No Hit
GCCCCCGACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCG20.16963528413910092No Hit
GTGGATAGGGGTGAAAGGCTAAACAAACTTGGAAATAGCTGGTTCTCTCC20.16963528413910092No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA20.16963528413910092No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.16963528413910092Clontech Universal Primer Mix Long (100% over 23bp)
CTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTG20.16963528413910092No Hit
CTCCCGGCCGATGGCCTCGTGGTTGATGGGGAAACGCGGGGTGCCGGCGT20.16963528413910092No Hit
CTGCATAGCTGCACGCTGAAGAACGGGGGCACATCAAGTATATCAGTCCC20.16963528413910092No Hit
TATAAATCCTGGTCTTCCATCTGGTGTTTTTGATGGAGGGACATATTTCT20.16963528413910092No Hit
CAGCAAGGAGCGTGGCCAGGTGTGGGTCGAGTGGTCCTACGCCGTGAACC20.16963528413910092No Hit
CTAGACTAATTGATCGGACCGATGACAGTTCACAGAGCATCCTTATACAG20.16963528413910092No Hit
ATCTAATTCACCTCCTATCAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA20.16963528413910092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.16963528413910092No Hit
GGTGATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTATT20.16963528413910092No Hit
GTCCTACGCCGTGAACCTGAAGCAGGAACTGCTGGATCCCACCGTCTTCG20.16963528413910092No Hit
CTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGT20.16963528413910092No Hit
TTATTGACTCTGTCACTCATTAGCATCTCCGAGGAAGTGTGACCAGGAGG20.16963528413910092No Hit
CCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATGGCGAATGACAGT20.16963528413910092No Hit
CTCGTGATGTGTGTCCTCAACTAAAGGAGTAGAACCTTTGTTTTCATAGA20.16963528413910092No Hit
GTATCAACGCAGAGTACATGGGATGGTTCAACGCAGAGTACATGGGAAGC20.16963528413910092No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG20.16963528413910092Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA20.16963528413910092No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGTCCGCCATATCAGAAATCGCATTCATGAAATCAA20.16963528413910092No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA20.16963528413910092No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG20.16963528413910092No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT20.16963528413910092No Hit
ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC20.16963528413910092No Hit
CCACCGACACGCCCTCGAGCTCGGCCTCGGGCCGCAGCTGGACCCGGTGG20.16963528413910092No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA20.16963528413910092No Hit
AACCCTGCCTAAGGGCACAACGATCAAGACCTTCTACGATCGTGGCAGCC20.16963528413910092No Hit
ATCTTTACCCGAGAAAGATATCTCTGTAAGTTTTCAAGCAACCGCGTCCT20.16963528413910092No Hit
CGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCA20.16963528413910092No Hit
AGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTC20.16963528413910092No Hit
CGCCTAATCTTTACCCGAGAAAGATATCTCTGTAAGTTTTCAAGCAACCG20.16963528413910092No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT20.16963528413910092Clontech Universal Primer Mix Long (96% over 25bp)
GTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTCAAT20.16963528413910092No Hit
GGAGTATTCTTCGTATGAGCCCGAGCCGTCTCCTGAGCCAGAGCCTACAT20.16963528413910092No Hit
GGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCATGTGTAGTGCGCCATA20.16963528413910092No Hit
TATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCC20.16963528413910092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.16963528413910092No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
GTTTAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20.16963528413910092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAAATGGAGTATATATTGAAGATACATTTGCAGAAGCATTCCCAATATGG20.16963528413910092No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
CCTTCAAGCATGAATTGTAGCTGACGGCAGGCCCCTAAACCCAAAAGTCG20.16963528413910092No Hit
AAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCC20.16963528413910092No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT20.16963528413910092Clontech SMART CDS Primer II A (96% over 26bp)
GTATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA20.16963528413910092No Hit
CCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGG20.16963528413910092No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
CCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCC20.16963528413910092No Hit
GCACAGGGAGCTTCATGTTGTGCCACAGGAAGTCCAAGAAAGCGGCGGCC20.16963528413910092No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC20.16963528413910092Clontech Universal Primer Mix Long (96% over 26bp)
GATAAGAACCCTCCAGCTCGGGGCCTCAAACCGATATTCCATGTGGGCCA20.16963528413910092No Hit
CATGGGAAGCATGGTGTCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16963528413910092No Hit
CCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGA20.16963528413910092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)