FastQCFastQC Report
Tue 10 May 2022
EGAF00003633391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17334
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1460.8422752971039575Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1090.6288219683858313Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.6057459328487366No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA830.47882773739471557No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT750.4326756663205262Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT600.3461405330564209Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.32306449751932614No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA480.27691242644513675No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG450.25960539979231567Clontech Universal Primer Mix Long (96% over 26bp)
CTCACGGTCGTGGGATACCTCCCAGTCGCGGTTTCTGTGGTAGTGGTTGC420.24229837313949465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.23652936425522095No Hit
TACACAATGTAGCCCACCACGAGCACCACCAGCGGCACGCCCGTGACCAG400.2307603553709473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.2307603553709473No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG390.2249913464866736Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC380.2192223376023999Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA380.2192223376023999No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC370.21345332871812622Clontech SMART CDS Primer II A (96% over 26bp)
TGCGCGGCGTGCCTTCGCGGCGCACTTCCACGTTCTGCGTCTCGTCGCGG370.21345332871812622No Hit
GATCGCTGATGACCGCTGCGCTGGCATAGACGGGCAGCGCTTGCCGATGT360.20768431983385255No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT350.20191531094957887Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCGCCAGCCGAAGCACGCGCGGCCTGGGGTTGGAACCCGGCGAGAACCC340.19614630206530517No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA340.19614630206530517No Hit
GACCAGCACCGTGGGCACAAGGGCCAGTCCCGCAAAGATCAGCACCAGCC330.1903772931810315No Hit
CGTCAGGCGCAGCACCTCGCTCCAGTTCTTCAGATGCTGGTGAGCACGCA320.1846082842967578No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT310.17883927541248412Clontech Universal Primer Mix Long (96% over 26bp)
CGTCCGGAGACGCCGCCACCAAAATATTCCGAGGCTCCGCACGCTCAGCC300.17307026652821045No Hit
GCTTTTAGGATGGCAAGGGACTTCCTGTAACAACGCATCTCATATTTGGA300.17307026652821045No Hit
GAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGT300.17307026652821045No Hit
GTGCCGGAGGCCCAGTCGTGGGACTCCACGAGGCCGACGGACAGGCCGCG290.16730125764393677No Hit
CCCCTGAAGAGATTACTTCTGTCCTATCTGATGATCCTGAATTATCAAGT290.16730125764393677No Hit
CGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATAC290.16730125764393677No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA290.16730125764393677Clontech Universal Primer Mix Long (95% over 21bp)
CTTCGTCGCGGATCAGGATGCGGGCCATGTTGTGGAAGGTCTGGCTCATG290.16730125764393677No Hit
GGTGGTGAGCGCGACGATGGTGGCTTACGGCGGCGGTACCGCGCTGGCGA290.16730125764393677No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC280.16153224875966307Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCCTACACCGTTACCGTCCGCGTCCGCGTCCGGTTCCTCCCACGCCGCG280.16153224875966307No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT280.16153224875966307Clontech Universal Primer Mix Long (96% over 26bp)
GCGTCACGCCCTGCGCATCGCCCAGGCTGGCGACATCCACGCCCATCAGC280.16153224875966307No Hit
GTGGAGGGTGTACGTGGTGTCGAGATTAAACCCGCGCATTCCCCTCGTGT270.1557632398753894No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT270.1557632398753894Clontech Universal Primer Mix Long (96% over 26bp)
TAGTGGAGTGGAGTGGAATGGAATGGGATGGAATGGAGTGGAGTAGAGTG270.1557632398753894No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT270.1557632398753894Clontech Universal Primer Mix Long (96% over 26bp)
CCATTCCATTTCACTCCACTCCACTCCATTCCATTCCACTCCACTCCACT270.1557632398753894No Hit
TCACAGCACACGGCGTGCGCCGCGAAGCTCGCGGATATTAAAGACCCCTG270.1557632398753894No Hit
GTTTCGGAGAATTCTTCTGTCTAGATTTATACGAAGAAATCCCGTTTCCA270.1557632398753894No Hit
GCATGTGCGTCACCGGCAGAACACGCCACACACACGGCGGCGAGTGCCAC260.14999423099111572No Hit
GTATCAGAGACACAGTGAGATATCACCTTTCCATAAATGCACTTCAGCAA260.14999423099111572No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT260.14999423099111572Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGACACCTCTCCGGTTCTTGCAGTCAAACTCCAGGAGTGTTTTGGCT260.14999423099111572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG260.14999423099111572No Hit
GAATGCAGGGGGACCTAACTTCGGCTGTCCGGCCTTTCCGAGTGTCACCT250.14422522210684205No Hit
GGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCATGTGTAGT250.14422522210684205No Hit
GATGCAAACTGTTTGACAGCCACGTTCTGCCAGATTCATAGGGAATGGAT250.14422522210684205No Hit
GTCCACGCCTTCGTCCTGCCACAGGCCGCGCAGCGCCTGGCCGAAGCGGT250.14422522210684205No Hit
ACCTTGGGGTGTTTCCACACCACATCAGAAAACGCCGTTGCGGGAAGATC250.14422522210684205No Hit
CCTTAAGGGGCGCGACCCCCATAACCTTGCCGCATCCTTCTTGTTTCGAC250.14422522210684205No Hit
CTCCGCGCGCATGTCGCCGCCCTCGTCCACCGACACCGTGATATATCCCA250.14422522210684205No Hit
GACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCC240.13845621322256838No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA240.13845621322256838Clontech Universal Primer Mix Long (96% over 26bp)
ACATTGGTCACCTGGCATGGGGTTTCCTTTCGGAAGTTAACCCCCGGCCC240.13845621322256838No Hit
GCAATTTCACCGGCGGCTTCCGGCAGCGGCTTGCCTTGCTCGGCCGTGAG240.13845621322256838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA240.13845621322256838No Hit
AAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCAC240.13845621322256838No Hit
ATTTCCAAGCGAGATATTTAGAGCGCCTTGAAGCCTATGCTAGAAATGGA230.13268720433829467No Hit
AGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTATATT230.13268720433829467No Hit
CCGCCACACCCACTGGGGGCGACAGCACCGAATCCCATAACCGCTCAGGC230.13268720433829467No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA230.13268720433829467No Hit
ACTCAGGGCTACCCGTGGGCAAAATACCCAACTCGGGTGAAGCCTCCGCG230.13268720433829467No Hit
ATTCTAGAGTTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGG230.13268720433829467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG230.13268720433829467No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC220.126918195454021Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTGTTAGCTACCAGGTTCCACTCATGGTGACGGGGTATCCAACACG220.126918195454021No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC220.126918195454021No Hit
GTAGCGTGGTCAACCCCGCCAGTACGGCCAGGGTGAGGAAGCTGGCATCC220.126918195454021No Hit
GTTCTGTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAA220.126918195454021No Hit
GGCGGCGGCGGTATAGGCGTCGGCGAGGCGGGCGGCGGCGGCGCGGTCCT220.126918195454021No Hit
CCACAGACCATGTACGGTCAGAAAATCAGCTTTGTTGGTCGTTTCGGTTT210.12114918656974732No Hit
GTTCTTATTCCTATCGTGGCAGAGTGCTTCGAGCTGAGCGGGGTTGTCGT210.12114918656974732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT210.12114918656974732No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG210.12114918656974732Clontech Universal Primer Mix Long (96% over 26bp)
CTCGCGGGCCTTCGCGAGGGAGCCGTCGATCACAGTTCGTTATGTCTTCG210.12114918656974732No Hit
GGATTAAGGCGCGATATGGCGCGAGCAGTGCTCTCTATTTCTTGGGGTTG210.12114918656974732No Hit
GGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.12114918656974732No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT210.12114918656974732Clontech Universal Primer Mix Long (96% over 26bp)
GTGCAGCGCCAGGTAGGCCTTCACTTTTTGCAATTCGACGGCGCGTTCTT200.11538017768547365No Hit
CGATGATGTGGGGCTGGCCGAAGTAGCCCAGGCCCCAGGCGAGGGCGGAG200.11538017768547365No Hit
GGTGCGGGACTCGAGGTCGATCCGCCAGGTGCCCTGGTCGGGGACCATCA200.11538017768547365No Hit
GATGGTGACAGGGGTACGTTTGTCATCGTGGAGACGATAGTAAAACCCAT200.11538017768547365No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG200.11538017768547365Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA200.11538017768547365No Hit
ACATGGGAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTA200.11538017768547365No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.11538017768547365No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA200.11538017768547365Clontech Universal Primer Mix Long (96% over 26bp)
GTTCCACACTAATCAAGAAGAGTGTTCAGATGTGAGGCCCAACCCAGGGG200.11538017768547365No Hit
GCATTAGGGGTCGCTGAGGCGATCCACAATTTACAGCTAGAAAAACTACC200.11538017768547365No Hit
GTGCCGGGGCGAGACATTTGGCCAGGCTAACCATCCGCTAACCCTGCATC190.10961116880119995No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG190.10961116880119995Clontech Universal Primer Mix Long (96% over 26bp)
CATCAATGCTTCGCGCAGCCTGCTGCCTTCGGGCCTGCCGAGCAACCCGA190.10961116880119995No Hit
GTACATGGGACGGCTGCAATTGAAGACTCCAGCAGCCTCTTCTCCTCTTT190.10961116880119995No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT190.10961116880119995Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGGCCCCGGGCCACTCCTGACTGGACCAGC190.10961116880119995No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA180.10384215991692627No Hit
CATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATT180.10384215991692627No Hit
GTCCTGGGCCATCAGTGTCTTGGCGCGGTCATAGCAATCAAGGGCCATAT180.10384215991692627No Hit
GCAGCGGCATGAACTGCGAGAACGGCAGGTTCTCCACCATGTCGAAGAGC180.10384215991692627No Hit
CCAAAGACCAGCGCCATGCCGTTGTCCAAGCCACGCTGCATGGCCGAGTC180.10384215991692627No Hit
GTGCGCGCTCCTCGTCCTGACGATCAGGTGATCCTCAAAGCTGACGGGTT180.10384215991692627No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)