FastQCFastQC Report
Tue 10 May 2022
EGAF00003633392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003633392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17334
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1500.8653513326410524Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.6749740394600208No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA960.5538248528902735No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT870.5019037729318103Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG780.4499826929733472Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC660.380754586362063Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG570.3288335064035999Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT560.32306449751932614Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA540.3115264797507788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.3057574708665052No Hit
GGGTATTTGAACGGCGAACTCTTCGCCATGCTGCTGCCGTTCATCGCCGG400.2307603553709473No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT380.2192223376023999Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG380.2192223376023999Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG380.2192223376023999No Hit
GGACAAATCCTCCCGCAACAAATAATCCCTACGGGCCGGTACGATAAAAT370.21345332871812622No Hit
ATGCTGGCGGGTGCCACGATGACGGTGCTTGGCCCGCTGGCGCAGGGCGC360.20768431983385255No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC360.20768431983385255Clontech Universal Primer Mix Long (96% over 26bp)
ACCACCGACCACTTACTCGACCACGGCCACTTACTCGACCACGGCCACGG360.20768431983385255No Hit
ATGCGGCTGCGTTCGGCAAACGCCTGTGCAACCGCTGAACAACGCTGTGC350.20191531094957887No Hit
CCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAACTACGAGCTTTTT340.19614630206530517No Hit
GCTCTCCGGCGATCGCCTCCATGGTCTGGCTCTGCCCGTAGTACATCCGT340.19614630206530517No Hit
ATCCTGGCTCGCTAGCGGGTTGTCTTGCTGTGCGCAGCTACGGTGGCAGT330.1903772931810315No Hit
CCCAATGCGCTGTCGCCCTCACACAGGTGTAGCTCTGCACCGCTGCCACG330.1903772931810315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.1903772931810315No Hit
TTGCGGGCCTGGGCGGGGATGACGCGCGCATCCTGACCTTCAACAGCCAG300.17307026652821045No Hit
GTCCAAGGCCGATCTGAACGTGGCCTCGCGCTACGCGCAGCTCTGCGACG300.17307026652821045No Hit
GCGGCGTATCGCGAGCGCAACCGCATCCTGAAGGCATCGGCGGCGCTGCG300.17307026652821045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA300.17307026652821045No Hit
GTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGG290.16730125764393677No Hit
TAGTATTTCCAAGCGGATATTTGGAACGCCTTGAAGCGTATGGCAGAAAA290.16730125764393677No Hit
GTGGTGGCACAATATCACCTCACATTTCATTTCACCATTGCTTTCAATTC290.16730125764393677No Hit
AGGTAATAGTTTGTTGGTCGTGTAGAATATCTGTTCGTAATCCGTCCCCT290.16730125764393677No Hit
GCGCCAAGGCGCTGCGCGACATGAACCTGCAAACGCACGTGGTCGAATTT280.16153224875966307No Hit
GCTGTTTTCGTGTATTTAAGCTTAGATTTTCAGTCGCTCGACAAATAAGG270.1557632398753894No Hit
GGTGTGTTCACTGTTCACAGGTTTTTGCGTTCATTATGCAGTTTCTTCGT270.1557632398753894No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT270.1557632398753894Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.1557632398753894No Hit
CAGGTACACCCACTCCGCCTCGCTCCGGCTCCGGCTCCACCCCGTCCACC270.1557632398753894No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA270.1557632398753894No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG270.1557632398753894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG270.1557632398753894No Hit
GGAGTGGCATGGAATTGGATGGTATGAAATGGATTGGATCGGAGAAGAGT260.14999423099111572No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG260.14999423099111572Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTGACCTGGTCATGTCTCCGCCGTAGTCTTTCTTTATCTGCTTTCTC260.14999423099111572No Hit
GTGTCTTCCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGG260.14999423099111572No Hit
GGACCCTATCAGAACTGAAAGGGGGGCCCTGGGAGGTGGGCCTTGGAGCC260.14999423099111572No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG250.14422522210684205Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT250.14422522210684205Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC250.14422522210684205No Hit
CAACCGGCGAGGTGGGGATGAAAGCGTCGTTGTTTGTGGCGGTGTGCCCG250.14422522210684205No Hit
CGCCCAACGGCATCGGCCCGCAGACACACTATGCGGCATACGCCGTGCCA250.14422522210684205No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT250.14422522210684205Clontech Universal Primer Mix Long (96% over 26bp)
ACATCAAGGTCGGCGACATGATCGCCTTCGACATCTCGCACCCGTGCCTC250.14422522210684205No Hit
GTGGTACGTAGTGGCATGAAGCTCGACGGCGACACGGTTGCTTTTGTACG250.14422522210684205No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT240.13845621322256838No Hit
ACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCAC240.13845621322256838No Hit
AGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACC240.13845621322256838No Hit
GTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTA240.13845621322256838No Hit
GTGTTGAGGAGGTTGCGCAGGCCAGCAAGGTTGTGGGGGATATTTTTCTT240.13845621322256838No Hit
ATGTAAAACTAGTTTCTATCAAATACATTTCTTTTCGGTGGCAGAGAATT240.13845621322256838No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT240.13845621322256838Clontech Universal Primer Mix Long (100% over 23bp)
ACCCACGGGATCGAAAACCAATTCACCACACGTATCTTGCCCCTGGAGGA240.13845621322256838No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC230.13268720433829467Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCC230.13268720433829467No Hit
CCACCGGCTCTGCCGACATGGACGGCACCGCCATCGGCGTTCTCGCGTTC230.13268720433829467No Hit
GTCCAGACACGCTCGCCCGATTCCATCATGCCCTTGGGCTTGTTCGCGTT230.13268720433829467No Hit
TAAATGGCCATTTGCATTCATGGTAACAACTCACAAGTATTAAAGGCTTA220.126918195454021No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT220.126918195454021Clontech Universal Primer Mix Long (96% over 26bp)
GATCCGCGCCCGCGAGGAGCGCTGGGCGGCGCTCGGCGGCGGCGCAGGGG220.126918195454021No Hit
TTCTAGCAGAGACTTCAAAGTGCTCTAAATATCCACTTGGGAATTCTACA220.126918195454021No Hit
GTCGTACCACGAACGTCGCGCACCAGCGAATCACTCGCCACGCACGGCAA220.126918195454021No Hit
GGTCAGATGTTGTTGGATCCGTTGGATTATCAGTTGGCGGCTGCTCGTAA220.126918195454021No Hit
GTACATGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAG220.126918195454021No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC210.12114918656974732Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGACAATAAATTGCTCAGGTTTCTTAGCTTGGTTCTGCCCTTAGCTCC210.12114918656974732No Hit
ATTGTTGACGGTCGAGCCTGTCGCGGTATAGGAGAGACAATAATACCGAT210.12114918656974732No Hit
GCTCACGACCGGCCTCTTCCTCGGTCCTCTGGCGGCGCGGCGATACGAGA210.12114918656974732No Hit
CTGCTACTTCGACCGTGGGGAAAAGCTAGGGACAGTTGTCACAGAAATGC210.12114918656974732No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA210.12114918656974732Clontech Universal Primer Mix Long (96% over 26bp)
GTTGAACCACTTGACCGAGCCCTGTGCCATACCTGTCACCTTCTGTGTCG210.12114918656974732No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG210.12114918656974732No Hit
TTCATCTCCTACGCAACGCTCGGCGTCACGGAGTTGGGCCAGGTGTATGA200.11538017768547365No Hit
GACTCTGGGTTCTTCATCTGCGTTCTAACGATGGTTAGGTTAATGCATCT200.11538017768547365No Hit
TCACCGGCGGGGACGGCGAGGGCGCGACGGCCGCGACCGCGCTGCTGAGC200.11538017768547365No Hit
CCTATGACGAGCATGTGACCACAGCACTGCTTGTGATCCGGAAGCGTTTG200.11538017768547365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA200.11538017768547365No Hit
GCGTAACGCCGCGTTGCTCGCGGTTTCTCTGCTTCCCTTCTCTTCTGCCA200.11538017768547365No Hit
GTTCTTAATTCTTCTCTAGAGTCAGAGGGAGGTCGCCTGCTGCCTGAGAA190.10961116880119995No Hit
ACCGTCGGGTAAGCCAGCAATTGAAGGAAATTACGCAACGCCTCAGGCAG190.10961116880119995No Hit
CTCTGTACCATCGACGGTGAAGTGGTCACCGGCACTAGCATTAGCAGATT190.10961116880119995No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT190.10961116880119995Clontech Universal Primer Mix Long (96% over 26bp)
GAAGGCGTGGGGGAAGAGCCAGGGCCCTGGGGAGCAGACGTCAGGGACTA190.10961116880119995No Hit
GAACATGGTTTCGCTTGTGAGTGTCGCTTCGAGGCGGCATTCTGGTCATC190.10961116880119995No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT190.10961116880119995Clontech Universal Primer Mix Long (96% over 26bp)
TCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC190.10961116880119995No Hit
CCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGACGCGGCAGTGCATA190.10961116880119995No Hit
GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG180.10384215991692627No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATG180.10384215991692627No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.10384215991692627No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA180.10384215991692627No Hit
CCTTCTACGTCTCCTCCGGCATGGTCGCCGGCGGCGTGTTCTTCCAGTCG180.10384215991692627No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCC180.10384215991692627No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)